T3 applied lectures

Question 1

in situ hybridization

Answer 1

mRNA found using complementary florescent probe

Question 2

DNA micro ray analysis

Answer 2

glass slides with genes can detect gene expression
process
- mRNA isolated
- reverse transcription
- florescents used to tag cDNA

Question 3

factors that contribute to RNA stability

Answer 3

1) length of poly A tail
2) micro RNA (miRNA)
- start as non-coding loop
- cleave into smaller products
- attach to RISC complex (RNA induced silencing complex)
INCOMPLETE BASE MATCH
3) small interfering RNA (siRNA)
- same proceses, but COMPLETE BASE MATCH, induce RNA cutting/cleavage

Question 4

factors influencing protein stability

Answer 4

selective degradation
- PROTESOMES: large pr- that break down uneeded/damaged pr-

UBIQUITIN PROTESOME DEGREDATION CASCADE
- unneeded pr- tagged with UBIQUITIN
- allows protesome to degrade pr