T#2 lab questions Flashcards

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1
Q

How will we measure activity of pyruvate dehydrogenase and the Krebs cycle in this week’s lab?

A.By measuring the volume of CO2 gas released by yeast.
B.By measuring the rate at which O2 is consumed by fish, snails and Elodea using an oxygen electrode.
C.By using titration with NaOH to measure the amount of CO2 produced by fish, snails and Elodea.
D.By using a pH meter to measure the change in acidity of the water that fish, snails and Elodea are living in.
E.By measuring the rate at which fish, snails and Elodea change the color of the water they are living in.

A

C.By using titration with NaOH to measure the amount of CO2 produced by fish, snails and Elodea.

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2
Q

The queen of England farted at Wimbledon. Attempting to blame it on the butler she said “stop that James!” James replied “very well Madam, but which way did it go?”
Please help James, which way did it go?

A.Upwind
B.Towards the highest concentration of humans
C.Up its concentration gradient
D. Down its concentration gradient
E. Away from all humans
A

D. Down its concentration gradient

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3
Q

How will we study glycolysis in this week’s lab?

A.By measuring CO2 released by fish
B.By measuring oxygen consumption by mitochondria
C.With a pH meter
D.By measuring fermentation by yeast
E.By measuring the change in glucose concentration of the media bacteria are growing in.

A

D.By measuring fermentation by yeast

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4
Q

What is the structure indicated by C (a blob on the outside of the lipid membrane on the inner part?

A.Transmembrane protein
B.Peripheral protein
C.Phospholipid
D.Carbohydrate
E.Cholesterol
A

B.Peripheral protein

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5
Q

Cells treated with inhibitors of the Na-K pump, Na ionophores or inhibitors of ATP synthesis can’t import ribose into their cytoplasm. How do they import ribose?

A.Receptor-mediated endocytosis.
B.Facilitated diffusion.
C.Channels
D.Antiport with sodium
E.Symport with sodium
A

E.Symport with sodium

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6
Q

How will we measure electron transport in this week’s lab?

A.With a Warburg respirometer.
B.By measuring the reduction of DCIP.
C.By measuring the volume of CO2 gas released by yeast.
D.With a pH meter.
E.By measuring the amount of weight lost by fish after an hour of aerobic exercise.

A

B.By measuring the reduction of DCIP.

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7
Q

Why do yeast grown anaerobically produce ethanol?

A.To keep millions of thirsty beer-drinkers happy.
B.To kill off bacteria competing with them for the same food sources.
C.To denature the proteins in their food in order to make them easier to digest.
D.To regenerate the NAD+ needed for the oxidation reactions of glycolysis.
E.To regenerate the ATP needed for the reduction reactions of glycolysis.

A

D.To regenerate the NAD+ needed for the oxidation reactions of glycolysis.

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8
Q

According to the second law of thermodynamics, which of the following is true?

A.Energy conversions increase the order in the universe.
B.The total amount of energy in the universe is constant.
C.The ordering of one system depends on the disordering of another.
D.The entropy of the universe is constantly decreasing.
All reactions produce some heat.

A

C.The ordering of one system depends on the disordering of another.

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9
Q

How can cells control their metabolism?

A. By actively transporting in substrates
B. By adding energy to their substrates
C. By controlling their enzymes
D. By changing their pH
E. By controlling their temperature
A

C. By controlling their enzymes

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10
Q

How will we measure CO2 uptake in this week’s lab?

A.By measuring the change in color of DCIP.
B.By measuring the decrease in CO2 with a CO2 electrode.
C.By measuring the decrease in CO2 with an infra-red gas analyzer.
D.By measuring the change in color of phenol red.
E.By titrating with NaOH to measure the change in CO2 concentration.

A

D.By measuring the change in color of phenol red.

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11
Q

Why does potassium chloride spontaneously dissolve in water even though this reaction is endothermic?

A.Potassium and chlorine both love water so they will dissolve whether the reaction is endothermic or not.
B.All endothermic reactions occur spontaneously.
C.The increase in entropy is so large that it results in a net decrease in the free energy of the system.
D.The decrease in entropy is so large that it results in a net decrease in the free energy of the system.
E.Substituting weaker hydrogen bonds between the potassium and chloride ions and water for the stronger ionic bonds between potassium and chloride ions lowers the free energy enough for the reaction to proceed.

A

C.The increase in entropy is so large that it results in a net decrease in the free energy of the system.

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12
Q

How is this biological pathway probably
controlled?

A. Substance 1 is an activator
of enzyme 4
B. The product is an activator
of enzyme 1
C. Substance 1 is a competitive
inhibitor of enzyme 4
D. The product is a competitive
inhibitor of enzyme 1
E. The product is a non-
competitive inhibitor of enzyme 1
A

E. The product is a non-

competitive inhibitor of enzyme 1

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13
Q

How will we measure the Hill reaction in this week’s lab?
A.By measuring the change in color of DCIP.
B.By measuring the release of oxygen in response to light.
C.By measuring the change in the absorption spectrum of chlorophyll.
D.By measuring the change in color of phenol red.
E.By measuring the uptake of CO2 by algae.

A

A.By measuring the change in color of DCIP.

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14
Q

Succinylcholine is almost identical to acetylcholine, but if mixed with the enzyme that hydrolyzes acetylcholine, the enzyme stops working. This suggests that:
A. Succinylcholine is an activator of this enzyme.
B. Succinylcholine is a noncompetitive inhibitor.
C. Succinylcholine is a competitive inhibitor.
D. The Ea for succinylcholine hydrolysis is higher than for acetylcholine hydrolysis.
E. The active site is the wrong shape to bind succinylcholine.

A

C. Succinylcholine is a competitive inhibitor.

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15
Q

How will we construct the absorption spectrum of photosynthetic pigments in this week’s lab?
A.By measuring the change in DCIP color as we change the light intensity.
B.By measuring the change in phenol red color as we change the wavelengths.
C.By measuring the amount of light absorbed as we change the wavelengths.
D.By separating the individual pigments using TLC.
E.By measuring the amount of CO2 absorbed as we change the light intensity.

A

C.By measuring the amount of light absorbed as we change the wavelengths.

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16
Q

Which of the following statements is TRUE?
A.One molecule of NADH contains more energy than one molecule of glucose.
B.One molecule of ATP contains more energy than one molecule of glucose.
C.Pyruvate is more oxidized than CO2.
D.Pyruvate is more reduced than glucose.
E.Two molecules of pyruvate contain less energy than one molecule of glucose.

A

E.Two molecules of pyruvate contain less energy than one molecule of glucose.

17
Q

What tissue will we use to study plant cell division in lab 7?

A. Onion root tip
B. Carrot Roots
C. Elodea leaves
D. Corn embryos
E. Rose petals
A

Onion root tip

18
Q

Where would you find Calvin Cycle enzymes?

A

In the stroma of the chloroplasts

19
Q

What tissue will we use to study animal cell division in lab 7?

A. Whitefish embryos
B. Cultured human cells
C. Frog eggs
D. Sea urchin gonads
E. Mouse liver
A

A. Whitefish embryos

20
Q

Which of the following are oxidized by beta oxidation?

Amino acids
Fatty acids
Nucleic acids
Sugars
ATP
A

Fatty acids

21
Q

What are the three stages of light reactions of photosynthesis?

A. PSI, PSII, Calvin cycle
B. Absorbing a photon, electron transport, ATP synthesis
C. Larry, Moe, Curly
D. Fixing CO2, reversing glycolysis, regenerating RuBP
E. Uv, visible, infrared

A

B. Absorbing a photon, electron transport, ATP synthesis

22
Q

What tissue will we use to study meiosis in lab 7?

Whitefish embryos
Lily pollen grains
Frog ovaries
Salmon testes
Fungal fruitnig bodies
A

Lily pollen grains

23
Q

What happens first inside a chloroplast when you turn off the lights?

Electron transport chain
Photorespiration stops
Carbon fixation stops
Glucose synthesis stops
ATP synthesis stops
A

Electron transport chain

24
Q

What is photorespiration?

A. Mitochondrial respiration that occurs in the light
B. Respiratioin by photographs
C. A form of respiration in which the final electron acceptor is a photon
D. Oxygen consumption by the light harvesting apparatus when the water-splitting enzyme works backwards in the dark
E. The addition of an O2 instead of a CO2 to RuBP by rubisco

A

E. The addition of an O2 instead of a CO2 to RuBP by rubisco