Synthetic Biology Flashcards
Top-down synthetic biology involves…
modifying the genetic makeup of an organism in order to produce desirable products
Bottom-up synthetic biology involves…
Synthetic molecule systems produced in order to carry our biological processes
Biobrick parts are DNA sequences containing _ and _ regions for insertion
functional sequences
enzyme-digestible
Devices are _ sets that perform _-cellular function
biobrick
sub-cellular
Systems are sets of _
devices
Synthetic biology has an emphasis on _ and _ design, it has a heavy reliance on _
standardization
rational design
synthetic DNA technology
Synthia strategy is to…
- Synthesize systems and combine into a synthetic genome in E.coli
- Propagate as a centromeric plasmid in yeast
- Transplanted into mycoplasma
- Tested for gene essentiality, redesign and cycle
CRISPR-Cas9 is a tool for _ by employing _ in order to selectively target specific sites
genome editing
gRNAs