Syllabus, Safety, Metric System and Measurement Flashcards
International System of Measurement is the
Metric System
Metric System Base Units are
Length: Meter (m)
Mass: Gram (g)
Volume: Liter (l)
**Temperature: Celsius (°C)
Length is
Meter (m)
Mass is
Gram (g)
Volume is
Liter (l)
To measure length we use
Ruler, calipers
To measure mass/weight we use
Balance
To measure volume we use
graduated cylinders, micropipettes, beakers
To measure temperature we use
Thermometers
What are the 3 types of micropipettes?
P20 = 2 µL-20 µL
P200 = 20 µL-200 µL
P1000 = 200 µL-1000 µL
“µ” Greek symbol Mu, means
Micro-
P20
First digit indicates 10s
Second digit indicates 1s
Third digit indicates a decimal (10ths)
Range: 2µL-20µL
P200
First digit indicates 100s
Second digit indicates 10s
Third digit is 1s
Range: 20µL-200µL
P1000
First digit indicates 1000s
Second digit indicates 100s
Third digit indicates 10s
Range: 200µL-1000µL
PRACTICE READING VOLUMES OF PIPETTES
What was done to make solutions in the first lab?
Weighing sucrose and dissolving it into Distilled Water (DI water)
spectrophotometers will
will emit a beam of light which will pass through the solution.
Spectrophotometer measures
Absorbance
Absorbance is
The quantity of light absorbed by a solution
What do we put into a spectrophotometer?
Cuvette
Ocular lens is ___ magnification
10x
Objective lens is ___ magification
4x , 10x, and 40 x
Total magnification
Ocular magnification (10x) X Objective Magnification
With magnification, always
Start with the red/scanning/4x objective