DNA BLAST Flashcards

1
Q

Chemical composition of the nucleus

Nucleon was not solely a protein due to its high concentration of phosphorus

A

Friedrich Miescher

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2
Q

Independently published papers on chromosome theory of inheritance
Genes are found at specific locations on chromosomes

NO DIRECT EVIDENCE

A

Walter Sutton and Theadore Boveri

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3
Q

Fruit flies provided direct evidence on chromosome theory of inheritance

A

Thomas Hunt Morgan

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4
Q

Heat-killed bacteria can transform a non-virulent strain — first evidence that genetic material is heat-stable chemical

A

Fredrick Griffith

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5
Q

Identify Griffiths’s transforming agent as DNA

DNA is capable of transforming the non-virulent strain of bacteria

A

Oswald Avery, Maclyn McCarty, Colon MacLeoed

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6
Q

DNA composition is species-specific
A=T
G=C

A

Erwin Chargaff

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7
Q

Bacteriophages grown in radioactive medium- DNA enters cells as the transforming agent

A

Alfred Hershey and Martha Chase

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8
Q

Spacial relationships of helical backbone
2 forms of DNA
Crystallography showed overall structure of DNA

A

Rosalind Franklin working in Maurice Wilkins’ lab

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9
Q

Compile info known about DNA and publish the DOUBLE HELIX with outer sugar phosphate-backbone and paired based facing forward

A

James Watson and Francis Crick

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10
Q

Transcription

A

RNA Synthesis
DNA -> RNA

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11
Q

Translation

A

Protein Synthesis
RNA -> Protein

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12
Q

Replication / DNA Synthesis is called

A

Semiconservative replication

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13
Q

Seem

A
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14
Q

Semiconservative replication

A

Replication where
2 strands of DNA that are genetically identical to the original strand

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15
Q

Transcription = RNA synthesis
To create proteins, we first create

A

Messenger RNA / mRNA

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16
Q

In RNA synthesis, Deoxyribose and Thymine are replaced by

A

Ribose and Uracil

17
Q

In translation / Protein synthesis,
The mRNA is converted into proteins by the

A

Ribosome (site of protein synthesis)

18
Q

3 letter codes

19
Q

MRNA is translated into __

A

amino acids / EACH CODON represents on animo acid

20
Q

GUA, UGC <- codons
Val, Cys - the names translated are__

A

Amino acids

21
Q

Amino acids together form a
Val-Cys-Lys-Ser-Leu

A

Polypeptide chain

Which FOLD into proteins

22
Q

Genetic code

A

Each codon will code for an amino acid

23
Q

Anime acids are __ of proteins

24
Q

In replication you make a copy of the DNA

A

In Transcription you make mRNA to make a complementary copy of the DNA strand

25
In translation you translate the generated mRNA into amino acids to make
Polypeptide chains
26
Lysis Buffer
Break down cells in our solution
27
Protease
Digest other proteins in the solution
28
Ice cold Ethanol
Separate DNA and make it Visable
29
Sickle Cell anemia
Folded cells block blood flow
30
Replication
DNA Synthesis DNA -> DNA