Subfertility Flashcards

1
Q

what is the WHO definition of infertility?

A

failure to achieve clinical pregnancy after 12 months or more of regular unprotected sexual intercourse (in absence of known reason) in a couple who have never conceived a child

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2
Q

where are sertoli cells found?

A

in the seminiferous tubules of the testes

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3
Q

which cell creates the blood testes barrier and what do this barrier do?

A

sertoli cells
protects the spermatozoa from antibodies and ensures the fluid composition within the testes is suitable for development of the sperm

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4
Q

what do the sertoli cells provide for the developing cells?

A

nutrients, destory defective sperm and remove excess cytoplasm from the seminiferous tubules

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5
Q

what do sertoli cells secrete?

A

seminiferous tubule fluid
androgen binding globulin
inhibin hormone
activin hormone

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6
Q

what does seminiferous tubule fluid do?

A

essential for carrying spermatozoa to epididymis

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7
Q

what does androgen binding globulin do?

A

essential for sperm production (binds testosterone)

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8
Q

what do inhibin and activin hormones do?

A

essential for regulation of FSH secretion and contorl of spermatogenesis

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9
Q

where does spermatogensis occur?

A

inside the seminiferous tubules

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10
Q

where are developing sperm stored?

A

epididymis

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11
Q

what is the vas deferens?

A

a muscular tube that transports te spermatozoe from the epididymis through the spermatic cord, through the inguinal canal, past the bladder and to join the seminal vesicle and forms the ejaculatory duct

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12
Q

what is the function of the bulbourethral gland (cowper’s gland)?

A

produces and releases fluid which lubricates urethra and neutralises any acidity, prior to ejaculation

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13
Q

what is the function of the seminal vesicle?

A

produces and releases majority of seminal fluid containing

  • fructose (nourishes sperm cells)
  • prostaglandins (triggers contraction of vaginal muscles for sperm motility)
  • fibrinogens (clot precursors)
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14
Q

what is the function of the prostate gland?

A

produces and releases

  • acid phosphatase
  • citric acid
  • inositol
  • calcium, zinc and magnesium
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15
Q

what is the hormonal control of spermatogensis?

A
  • GnRH is released in bursts every 2-3 hours from the hypothalamus
  • GnRH stimulates the Ant Pit to release LH and FSH
  • LH stimulations testosterone secretion from leydig cells
  • testosterone and FSH surges stimulate spermatogenesis with seminiferous tubules
  • inhibin released from sertoli cells decreases secretion of FSH and testosterone released from the Leydig cells decreases the release of GnRH adn therefore, decreases LH, providing negative feedback, inhibiting spermatogenesis
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16
Q

what is capacitation?

A

the series of biochemical and electrical events that take place to allow the sperm cell to penetrate the cell layer that surrounds the oocyte, by giving the sperm cell the ability to bind to the zona pellucida via the acrosome reaction

17
Q

why does cystic fibrosis cause male infertility?

A
  • obstruction or absence of the vas deferens bilaterally, known as CBAVD where the VD failed to develop properly
18
Q

what is cryptochidism?

A

one or both testes being undescended into the dependant part of the scrotal sac

19
Q

what is klinefelter’s syndrome?

A

47 XXY
genetical male with no extra X chromosome - developmental delays, reduced facial hair, poo muscle tone + gynaecomastia
- infertile

20
Q

what is a robertsonian translocation?

A

affects the acrocentric chromosomes (13, 14, 15, 21, 22), and can result in extra or missing chromosomes
- increases risk of infertility and miscarriage

21
Q

what is the triad for PCOS?

A

anovulation
polycystic ovaries
hyperandrogenism (clinical or biochemical)

22
Q

what are uterine polyps?

A

occur when the endometrial lining inside the uterus overgrows and as a result a polyp forms inside the uterus
- abnormal uterine bleeding
removed by hysterectomy

23
Q

what are uterine fibroids?

A

benign tumours of the myometrium that can grow in the uterus
main types: subserosal, intramural and submucosal
removed surgically by myomectomy

24
Q

describe intrauterine insemination (IUI)?

A

directly places the sperm inside the uterus to facilitate fertilisation
only possible when the sperm is healthy, ovulation is taking place and there is no tubal disease

25
Q

when would you use intrauterine insemination (IUI)?

A
sexual dysfunction (ejaculation dysfunction, erectile dysfucntion, sexual pain disorders) 
female same sex relationships (donor sperm) and male same sex relationships (surrogacy)
26
Q

what are the 4 steps involved in IVF/ ICSI?

A
  1. down regulation
  2. ovarian stimulation
  3. oocyte collection
  4. fertilisation
27
Q

what is in involved in down regulation?

A

synthetic GnRH analogues are given to ‘shut down’ the natural menstrual cycle, causing a temporary artificial menopause
this enables IVF cycles to be schedules and manages
TVUS carried out to ensure endometrium is thin and ovaries are inactive

28
Q

what is involved in ovarian stimulation?

A

inducing ovarian follicular development and oocyte maturation, by administering gonadotrophin hormone as an injection
it also helps endometrial wall to thicken in preparation for implantation of the embryo
GnRH agonist is used to prevent LH surges and prevent follicles from releasing the oocyte before it fully matures

29
Q

what is involved in oocyte collection?

A

it takes place in theatre under US guidance
the needle is inserted transvaginally and the ovarian follicles are aspirated, collecting the oocyte
they’re brought to the embryology lab where the embryologist uses a microscope to identify the oocytes = places in a culture and incubated at 37degrees

30
Q

how does fertilisation occur in IVF?

A

fertilisation
IVF - sperm places in petri dish with the oocyte = the successful sperm undergo capacitation and fuses with the zona pellucida, eventually the fertilising ovum and creating the zygote

31
Q

what are the indications for IVF?

A
unexplained fertility (longer than 2 years) 
pelvic disease (tubal disease, endometriosis, fibroids etc) 
anovulatory infertility (after failed ovulation induction
32
Q

how does fertilisation occur in ICSI?

A

one sperm cell is imobilised and injected directly into the cytoplasm of the oocyte, bypassing stages of the sperm travelling to the ovum, undergoing capacitation, the acrosome reaction and fusing with the ovum

33
Q

what are the indications for ICSI?

A

severe male factor infertility, previous failed fertilisation with IVF, pre-implantation genetic diagnosis

34
Q

what is surgical sperm aspiration?

A

used in male fertility with little or no sperm in the semen

35
Q

in surgical sperm aspiration, where the the sperm extracted from?

A

obstrucitve cause eg CF= epidydmis

non-obstructive = testicular tissue

36
Q

what is stage 5, embryo transfer?

A

occurs on day 5 (blastocyst stage)
embryo is inserted into the uterus, progesterone suppositories then used for 2 weeks
pregnancy test performed 16 days after oocytre recovery

37
Q

what causes ovarian hyper-stimulation syndrome?

A

occurs in response to the ovarian stimulation medications given in stage 2 of IVF and ICSI protocols

38
Q

how does ovarian hyper-stimulation syndrome occur and what are the symptoms?

A

large numbers of growing follicles and high oestradiol levels can lead to release of inflammatory markers and vasoactive substances
these cause fluid shift from within the blood vessels to the 3rd compartment leading to fluid build up in the abdomen
bloating, abdominal distension, nausea and vomiting