stupid micro bugs we should know

Question 1

characteristic biochemicals for enterobacter cloacae
- TSI
- ox
- motility
-indole
- citrate
- VP
- LDC
- urea

Answer 1

TSI- acid/acid
Ox- neg
motility - pos
indole - neg
citrate - pos
VP - pos
lysine decarboxy - neg
urea - neg

Question 2

biochemicals for plesimonas shigelloides that differentiate from the rest of the enterbacterales

Answer 2

pos oxidase

Question 3

biochemicals for Klebsiella pneumoniae
- KIA
- LDC
- indole
- citrate
- motility
- VP

Answer 3

KIA - acid/acid with gas
Lysine decarboxy - pos
indole - neg
citrate - pos
nonmotile
VP - pos

• opp path, necorsis or hemorrhage

Question 4

biochemicals for klebsiella oxytoca
- KIA
- LDC
- indole
- citrate
- motility
- VP

Answer 4

KIA - acid/acid with gas
lysine decarboxy - pos
indole - pos
citrate - pos
nonmotile
vp - pos

• opp path causing GI problems

Question 5

biochemicals for salmonella typhimurium
- KIA
- LDC
- ODC
- indole
- citrate

Answer 5

KIA - K/A with h2s
Lysine decarboxy (LDC) - pos
ODC - neg
indole - neg
citrate - neg

Question 6

biochemicals for yersinia enterocolitica
- gram
- KIA
- ONPG
- ODC
- urea
- motlity

Answer 6

gram - GNCB w/ bipolar staining
agar - CIN selective
KIA - K/A (orange)
ONPG - pos
ODC - pos
urea - pos
motility - motile at room, non motile at 37

• gastroenteritis

Question 7

biochemicals for escherichia coli
- gram
- indole
- methyl red
- VP
- citrate
- lysine
- motility

Answer 7

GNB LF
indole - pos
methyl red - pos
VP - neg
citrate - neg
lysine - pos
motility - pos

• most common UTI and kidney infection

Question 8

biochemicals for N. gonorrhoeae
- gram
- ox
- carb usage
- Dnase
- catscreen
- PRO

Answer 8

GNDC intracellular
ox - pos
glucose/dextrose - pos
maltose - neg
sucrose - neg
lactose - neg
Dnase - neg
catscreen - neg
PRO - pos

Question 9

biochemicals for Neisseria meningitidis
- gram
- Ox
- glucose
- maltose
- dnase
- cat screen
- GLUT

Answer 9

GNDC intracellular
ox - pos
glu/dex - pos
maltose - pos
DNase - neg
cat disk - neg
GLUT - pos

Question 10

biochemicals for Neisseria lactamica
- grma
- ox
- glucose
- maltose
- lactose
- BGAL

Answer 10

GNDC (opp but commensal)
ox - pos
dex/glu - pos
malt - pos
lactose - pos
BGAL - pos

Question 11

biochemicals for moraxella catarrhalis
- gram
- carb use
- dnase
- nitrate reduction- cat disk
- indolcyl butyrate

Answer 11

GNDC (hockey puck on agar)
ox - pos
carb use - all neg
Dnase - pos
nitrate reduction - pos
butyrate esterase (cat disk) - pos
indolxyl butyrate - pos

Question 12

biochemicals for actinobacter
- gram
- ox
- motlilty
- glucose

Answer 12

GNCB diplo
ox - neg
nonmotile
baumanii - glucose ox
lwoffii - glu non ox
will grow on mac and emb

Question 13

biochemicals for morganella morganii
- gram
- indole
- odc
- urea
- motility
- pda
- h2s

Answer 13

urinary tract infection
GNB non LF
indole - pos
ornithine decarboxy (ODC) - pos
urease - pos
motility - pos
PDA - pos
h2s - neg

Question 14

biochemicals for proteus mirabilis
- gram
- KIA
- TSI
- glucose
- indole
- urea
- tda
- h2s
- odc

Answer 14

GNB urine (swarming)
KIA - K/A
TSI - K/A
glucose - pos
indole - neg
urea - pos
TDA - pos
H2S - pos
ODC - pos

Question 15

biochemicals for proteus vulgaris
- gram
- kia
- tsi
- odc
- indole
- urea

Answer 15

UTI
GNB urine (swarming)
KIA - K/A
TSI - A/A (sucrose)
ODC - neg
indole - pos
urea - pos

Question 16

biochemicals and trasport for francisella tularensis
- gram
- ox
- cat
- glucose
- motility

Answer 16

causes tularemia, transport with cysteine (charcoal yeast extract)
GNCB
ox - neg
cat - neg
glucose - fermenter
non motile

Question 17

biochemicals for pseudomonas aeruginosa
- gram
- ox
- gluose
- acetamide
- arginine dihydrolas
- nitrate
- indole

Answer 17

pyocyanin producing
non fermenter
nosocomial infection
GNB
ox - pos
glucose - ox
acetamide - pos
growth at 43
Arginine dihydrolas - pos
nitrate pos
indole - neg