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BOC study (harr book) > micro externship 8.1 > Flashcards

micro externship 8.1 Flashcards

1
Q

the aseptic collection of blood cultures requires that the skin be cleansed with:
A. 2% iodine and then 70% alcohol solution
B. 70% alcohol and then 2% iodine or an iodophor
C. 70% alcohol and then 95% alcohol
D. 95% alcohol only

A

B. 70% alcohol and then 2% iodine or an iodophor

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2
Q

when cleansing the skin with alcohol and then iodine for the collection of blood culture, the iodine (or iodophor) should remain intact on the skin for at least
A. 10 seconds
B. 30
C. 60
D. 5 minutes

A

C. 60 seconds

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3
Q

what is the purpose of adding 0.025% to 0.050% sodium polyanethol sulfonate (SPS) to nutrient broth media for the collection of blood cultures
A. it inhibits phagocytosis and complement
B. it promotes formation of a blood clot
C. it enhances growth of anaerobes
D. it functions as a preservative

A

A. it inhibits phagocytosis and complement

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4
Q

a flexible calcium alginate nasopharyngeal swab is the colelciton device of choice for recovery of which organism from the nasopharynx
A. staphylococcus aureus
B. strep pneumo
C. Corynebacterium dipheriae
D. bacteroides fragilis

A

C. corynebacterium diphtheria

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5
Q

semisolid, such as Amies, Stuart, or Cary-Blair, are suitable for the transport of swabs for culture of most pathogens except
A. Neisseria gonorrhea
B. Enterobacteriaceae
C. campylobacter fetus
D. streptococcus pnemoniae

A

A. Neisseria gonorrhea

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6
Q

select the method of choice for recovery of anaerobic bacteria from a deep abscess
A. Cotton fiber swab of the abscess area
B. skin snip of the surface tissue
C. needle aspirate after surface decontamination
D. swab of the scalpel used for debridement

A

C. needle aspirate after surface decontamination

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7
Q

select the primary and differential media of choice for the recovery of most fecal pathogens
A. MacConkey, blood, birdseed and Campy
B. hektoen, Mac, mac-sorb, campy blood, CNA and SEL
C. CNA and christensen urea agars and thio media
D. blood, campy, mueller-hinton and thio

A

B. Hektoen, MacConkey, MacConkey-Sorbitol, campy blood, colistin-nalidixic acid and Selenite-F broth

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8
Q

select the media of choice for recovery of vibrio cholerae from a stool specimen
A. MacConkey agar and thio media
B. Thiosulfate-citrate-bile-sucrose (TCBS) agar and alkaline peptone water broth
C. blood agar and SEL broth
D. CNA agar

A

B. TCBS and APW

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9
Q

CNA agar is used primarily for the recovery of:
A. Neisseria species
B. Enterobacteriaceae
C. pseudomonas aeruginosa
D. staphylococcus aures

A

D. staphylococcus aureus
- CNA inhibits GN and isolates GPC

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10
Q

in the united states, most lood agatr plates are prepared with 5% or 10% RBCs obtained from
A. sheep
B. horses
C. humans
D. dogs

A

A. sheep

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11
Q

all of the following are appropriate when attempting to isolate N. gonorrhea from a genital specimen except
A. transport the genital swab in charcoal transport medium
B. plate the specimens directly onto MTM medium
C. plate the specimen directly on New York City or Matin-Lewis agar
D. culture specimens in ambient oxygen at 37

A

D. culture specimens in ambient oxygen at 37

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12
Q

chocolate agar and MTM agar are used for the recovery of
A. haemophilus spp and neisseria spp respectively
B. haemophilus spp and N. gonorrhea respectively
C. Neisseria spp and streptococcus spp respectively
D. streptococcus spp and staphylococcus spp respectively

A

B. Haemophilus spp and N. gonorrhoeae

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13
Q

CCFA is used for the recovery of
A. yersinia enterolitica
B. yersinia intermedia
C. clostridium perfringens
D. clostridium difficile

A

D. clostridium difficile

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14
Q

DCA is useful for the isolation of
A. Enterobacteriaceae
B. enterococcus spp
C. staphylococcus spp
D. neisseria spp

A

A. enterobacteriaceae

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15
Q

XLD agar is highly selective medium used for the recovery of which bacteria
A. staphylococcus spp from normal flora
B. yersinia spp that do not grow on hektoen agar
C, Enterobacteriaceae from gastrointestinal specimens
D. streptococcus spp from stool cultures

A

C. Enterobacteriaceae from gastrointestinal specimens

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16
Q

a sheep blood agar plate is used as a primary isolation medium when all of the following organisms are to be recovered from a wound specimen except
A. beta hemolytic strep and coag pos taph
B. haemophilus influenza and haemophilus parainfluenza
C. proteus spp and E coli
D. pseudomonas and acinetobacter

A

B. Haemophilus influenzae and Haemophilus parainfluenzae

17
Q

preredurced vitamin K supplemented blood agar plates are recommended isolation media for
A. mycobacterium marinum and mycobacterium acium intracellular
B. bateroides, peptostreptococcus and clostridum
C. proteus
D. enterococcus

A

B. B. bateroides, peptostreptococcus and clostridum

18
Q

which procedure(s) is (are) appropriae for the diagnosis of Chlamydia spp infections when using genital specimens
A. obtain urethral, cervical swabs and urine specimens placed in transport media for the direct detection of antigen or nucleic acid and/or culture
B. plate onto blood and chocolate agar
C. inoculate thio broth
D. late onto MTM agar within 24 hours

A

A. obtain urethral, cervical swabs and urine specimens placed in transport media for the direct detection of antigen or nucleic acid and/or culture

19
Q

specimens for virus culture should be transported in media containing
A. antibiotics and 5% sheep blood
B. saline and 5% sheep blood
C. 22% bovine albumin
D. antibiotics and protein nutrient

A

D. antibiotics and protein nutrient

20
Q

CSF should be culutred immediately but if delayed the specimens should be
A. refrigerated
B. frozen
C. stored at room temp for no longer than 24 hours
D. incubated at 37C and cultured as soon as possible

A

D. incubated at 37C and cultured as soon as possible

21
Q

the most sensitive method for the detection of beta-lactamase in bacteria is by the use of
A. chromogenic cephalosporin
B. penicillin
C. oxidase
D. chloramphenicol acetyltransferase

A

A. chromogenic cephalosporin

22
Q

the breakpoint of an antimicrobial drug refers to
A. the amount needed to cause bacteriostatic
B. a minimum inhibitory concentration of 16 ug/mL or greater
C. an MIC of 64 ug/mL or greater
D. the optimal therapeutic level of drug that is achievable in serum

A

D. the optimal therapeutic level of drug that is achievable in serum which

23
Q

of the following variable may change the results of an MIC
A. inoculum size
B. incubation time
C. growth rate of the bacteria
D. all of these

A

D. all of these

24
Q

according to the Kirby Bauer standard anti-microbial susceptibility testing method, what should be done when interpreting the zone size of a motile, swarming organism, such as a Proteus spp
A. the swarming area should be ignored
B. the results of the disk diffusion method are invalid
C. the swarming area should be measured as the growth boundary
D. the isolate should be retested after diluting to a 0.05 McFarland standard

A

A. the swarming area should be ignored

25
Q

which class of antibiotics is used for the treatment of serious -negative infections as well as infections with Mycobacterium tuberculosis
A. cephalosporins
B. penicillins
C. tetracyclines
D. aminoglycosides

A

C. aminoglycosides

26
Q

select the medium best suited for the recovery of Y. enterolitica from a patient with gastroenteritis
A. Hektoen agar
B. CIN agar
C. blood agar
D. eosin methylene blue agar

A

B. CIN agar

27
Q

a suspected case of plague requires which of the following procedures to confirm Yersinia pestis
A. collection of multiple blood culture specimens
B. incubation of blood cultures at 28C and 35 C
C. culture aspirates from bubos to MacConkay agar at room temp
D. all of the above

A

D. all of the above

28
Q

SITUATION: abdominal pain, fever, vomiting, and nausea prompted an older male to seek medical attention. A watery stool specimen producing no fecal leukocytes or erythrocytes was cultured, and it grew a predominance of gram-negative fermentation bacilli. The colonies were beta-hemolytic on blood agar and cream-colored on MacConkey agar. The colonies were both oxidase and catalase-positive. what is the most likely identification
A. Aeromonas hydrophilia
B. Escherichia coli
C. Salmonella spp
D. Shigella spp

A

A. Aeromonas hydrphilia

29
Q

SITUATION: Several attendees of a medical conference in the Gulf coast area became ill after frequenting a seafood restaurant. A presumptive identification of V. cholerae was made after stool specimens from several subjects grew clear colonies on MacConkey agar and yellow colonis on TCBS agar. Which key tests would help eliminate Aeromonas and Plesiomonas
A. mannitol fermentation, Na requirement
B. oxidase, motility
C. Oxidase, nitrate
D. hemolysis on blood agar, catalse

A

A. mannitol fermentation, Na requirment

30
Q

SITUATION: a group of elementary students became ill after eating undercooked ground beef prepared in the school cafeteria. The suspected pathogen, E. coli 0157:H7, is usually recovered using which of the following media
A. Xylose lysine deoxycholate agar
B. MacConky agar
C. MacConkey agar with sorbitol
D. Hektoen agar

A

C. MacConkey agar with sorbitol

BOC study (harr book) (16 decks)

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