Studying Gene Expression and Molecular Genetic Techniques Flashcards
RNA-seq
analyse a large number og cDNA from a tissue and the use the shotgun sequence analysis (The method involves randomly breaking up the genome into small DNA fragments that are sequenced individuall)
RNAi (RNA interference)
double stranded-RNA which can block expression of its single-stranded mRNA but not that of mRNA with a different sequence
CRISPR
Clustered regulaly interspaced short palindromic repeats genome editing technique.
DNA microarray
a glass microscope slide with organized, individual closely packed sequences at around 20 nucleotides long
method 1: pcr of DNA segments that is rhen atach to the glass surface and then denature them
method 2: macking smal oligos where the first nucletide is attach to the glass. can costumize the oligos then synthesizing them
hybridization
DNA/RNA´s ability to specifically associate with each other via baseparing because they are complementary singlestranded
in situ hybridizatin
Detection of one mRNA in a spatially and temporally defined manner
“In situ hybridization is a highly sensitive technique that allows detection and localization of specific DNA or RNA molecules in morphologically preserved isolated cells, histological tissue sections, or chromosome preparations”
single cell RNA-seq
RNA-seq of a single cell. can show expression of individual cells mRNA
(transiet/stable) transfection
“transfection is a modern and powerful method used to insert foreign nucleic acids into eukaryotic cells.”
transient Transfection: the constructed plasmids are introduced into mammalian cells in some ways, but the foreign genes of the plasmid are not integrated into the genome of the mammalian cell”
Stable transfection: “sustaining long-term expression of a transgene by integrating foreign DNA into the host nuclear genome”
Cre/LoxP
