Structures and functions in living organisms Flashcards
What is the order of level of organisation
Organelles
Cells
Tissues
Organs
Organ systems
Describe each level of organisation
Organelles: specialized subcellular structures found within living cells
Cells : basic structural unit of living organisms
Tissue : groups of cells, either similar structures working together to perform the same function
Organs : groups of tissues, working together to perform specific functions
Organ systems : groups of organs with similar functions, working together to perform body functions
Function of nucleus
• contains genetic material
• controls cell activity
Function of cytoplasm
• liquid substance where chemical reactions occur
• contains enzymes
Function of cell membrane
• controls what enters and leaves the cell
Function of mitochondria
• where aerobic respiration reactions occur
• provides energy for the cell
Function of ribosomes
• where protein synthesis occurs
Function of chloroplast
• where photosynthesis takes place
• contains chlorophyll pigment, which harvests light needed for photosynthesis
Function of permanent vacuole
• contains cell sap
• found within cytoplasm
• improves cells rigidity
Function of cell wall
• provides strength to cell
• made from cellulose (in plants)
• made from chitin (in fungi)
Biological molecules:
Carbohydrates
Proteins
Lipids
What are carbohydrates
• made of carbon, oxygen, and hydrogen
• are polymers that break down into simple sugars
What are proteins
• made of carbon, oxygen, hydrogen, sulfur, nitrogen and phosphorus
• are polymers that break down into its monomer : amino acids
What are lipids
• made of carbon, oxygen, and hydrogen
• large polymers that break down into 3 fatty acid molecules and a glycerol molecule
Test for glucose
- Add sample solution into test tube
- Add drops of Benedicts solution into test tube
- heat in water bath at 60-70°C for 5 mins
- take test tube out and record colour
POSITIVE = Brick red
NEGATIVE = remains blue
Test for starch
- Use pipette and add sample solution onto tile
- Add drops of iodine solution and
- Leave for 1 minute and record colour change
POSITIVE = Blue-black
NEGATIVE = remains brown
Test for protein
- Add sample solution into test tube
- Add drops of Buirets solution into test tube 3. Leave for 1 minute and record colour change
POSITIVE = purple
NEGATIVE = remains blue
Test for fats
- Add 2cm³ of ethanol to test solution
- Add 2cm³ of distilled water
- Leave for 3 minutes and record colour change
POSITIVE = milky white emulsion formed
NGATIVE = remains colourless
Role of enzymes
Biological catalysts
Enzymes are proteins that help speed up chemical reactions in our bodies.
Effect of temperature on enzymes activity
•optimum is 37°C
•rate of reaction increases with increase in temperature until optimum
•above optimum, rate of reaction rapidly decreases and eventually reaction stops
• when temperature becomes too hot the bonds break
• This changes shape of active site, so substrate can’t fit
• enzymes is denatured and can’t work anymore
Practical : investigate how enzyme activity can be affected by changes in temperature
- Starch solution is heated to set temperature
- Amylase is added
- Iodine is added to each tile after a minute
- measure time taken until iodine stops going blue-black
- repeat with different temperatures
Effect of pH on enzymes activity
• optimum pH is 7 (most of the time)
• if pH is too high or low, forces that hold amino acid chains will be affected
• This changes the shape of the active site, so substrate doesn’t fit
• enzyme is denatured
Describe diffusion
• The net movement of particles from high to low concentration
• Passive process (no energy required)
Describe osmosis
• The movement of water from a less to higher concentration through partially permeable membrane
• high water potential to low water potential = down concentration gradient
• Passive process