Structure and Replication of DNA (2) Stages of DNA Replication Flashcards
What is the first stage of DNA Replication?
The original molecule of DNA unwinds out of its double helix structure.
What is the second stage of DNA Replication?
After the molecules unwind the hydrogen bond between the base pairs break allowing the two strands to form two template strands.
What is the third stage of DNA Replication?
After the hydrogen bonds are broken and the DNA is separated primers attach to the end of each exposed DNA template strand in order to start replication.
What is the fourth stage of DNA Replication?
DNA Polymerase can only add ‘free’ DNA Nucleotides which are present in the nucleus and the nucleotides can only be added in the direction of the 3’ end of the growing strand.
What is the fifth stage of DNA Replication?
Primers are added to the lagging strand and are added as the fork widens with the DNA Nucleotides being added in fragments, the enzyme ligase is then used to join the two DNA fragments together forming a complete strand.
What is the leading strand?
It is the strand that from the direction of replication goes from the 5’ end to the 3’ end.
What is the lagging strand?
It is the strand that from the direction of replication goes from the 3’ end to the 5’ end.
What is the sixth stage of DNA Replication?
The nucleotides align themselves to their complementary base pairs.
What is the seventh stage of DNA Replication?
New hydrogen bonds are formed between the newly formed base pairs and the chemical bonds form between the nucleotides creating the sugar phosphate backbone.
What is the eighth stage of DNA Replication?
The leading strand is replicated continuously and the lagging strand is replicated discontinuously in fragments.
What is the ninth stage of DNA Replication?
The two new daughter DNA molecules rewind to create two double helixes.
