Gene Expression (3) Flashcards

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1
Q

What is a genotype?

A

Is the combination of genes that a cell contains.

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2
Q

How is a cell’s genotype determined?

A

The sequences of bases in the genes determines the genotype of a cell.

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3
Q

What is a phenotype

A

A phenotype is the physical expression of the genes in a cell.

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4
Q

How is a cell’s phenotype determined?

A

It is determined by the proteins that are synthesised when the genes are expressed.

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5
Q

How much of the genes in a cell are expressed?

A

Only a fraction of the genes in a cell are expressed.

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6
Q

What affects gene expression?

A

The influence of environmental factors whether it be inside or outside (intra or extra-cellular).

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7
Q

What controls gene expression?

A

Gene expression is controlled by the regulation of the processes of transcription and translation.

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8
Q

What is a protein made up of?

A

One or more polypeptide chains.

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9
Q

What does RNA (Ribonucleic acid) do?

A

Provides a bridge between DNA and protein synthesis.

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10
Q

Where is RNA found?

A

Nucleus and Cytoplasm.

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11
Q

What are the three different forms of RNA?

A

rRNA
tRNA
mRNA

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12
Q

What does rRNA (ribosomal RNA) do?

A

It binds to proteins to form ribosomes which are the site of protein synthesis.

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13
Q

What does mRNA (messenger RNA) do?

A

It carries a copy of the DNA code from the nucleus to the ribosome.

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14
Q

What does tRNA (transfer RNA) do?

A

It pick up and carries a specific amino acid from the cytoplasm and carries it to the ribosome.

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15
Q

What is RNA in term of structure?

A

It is a single stranded molecule that is composed of nucleotides, which just like DNA contain a phosphate, a base and a sugar.

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16
Q

What is an RNA nucleotide made up of?

A

Base, ribose sugar and a phosphate.

17
Q

What are the four bases that an RNA nucleotide can contain?

A

Adenine, uracil, cytosine and guanine

Meaning there are 4 different kinds of nucleotides.

18
Q

What are the base pairing rules in RNA?

A

Adenine - Uracil
Guanine - Cytosine

19
Q

What holds the bases together in an RNA nucleotide?

A

Weak hydrogen bonds.

20
Q

What holds the nucleotides together in RNA?

A

Strong sugar (ribose) phosphate bonds.

21
Q

What are amino acids?

A

They are the building blocks of proteins.

22
Q

How many amino acids are there that exist naturally within cells?

A

20.

23
Q

How many bases is DNA made up of?

A

4

24
Q

How many amino acids could be coded for with the bases in DNA?

A

4 which would not be enough as when given a two letter code it would only result in 16 possible arrangements which is not enough as there are 20.

25
Q

What is a triplet code?

A

A triplet code is a code consisting of 3 bases.

26
Q

How many variations of amino acids can a triplet code produce?

A

64 which is more than enough as the required amount is 20.

27
Q

How many bases/nucleotides code for an amino acid?

A

3.

28
Q

What are the 2 main processes that take place during protein synthesis?

A

Transcription and Translation.

29
Q

What is the first step in protein synthesis?

A

Transcription.

30
Q

What does the process of transcription require?

A

DNA (to act as a template for the new RNA molecule)
RNA polymerase
A supply of four different nucleotides
ATP