streptococci Flashcards
classification based on temperature
academic/bergey’s classification
complete hemolysis?
culture?
beta-hemolytic streptococci
clear zone
classification based on hemolytic patterns
smith and brown classification
classification based on extraction of c-cho from streptococcal cell wall
lancefield classification
group which grows only at 37 C
pyogenic group
grow only at 37c and 45c
viridans group
all streptococci contains what antigen which is responsible for serological classfication
C-carbohydrate
grp of streptococci which is transmitted thru contaminated droplets by cough or sneeze
group A streptococci
enzyme produced by s. pyogenes which is oxygen labile
streptolysin O
partial hemolytic hemolysis?
culture?
alpha-hemolytic streptococci
green hemolysis
no lysis?
culture?
gamma-hemolytic streptococci
no change
nagpauso ng lancefield classification
rebecca lancefield
c-cho can be extracted from streptococcal cell wall by placing the organisms in ___ & __
dilute acid and heating for 10 mins
fever producing bacteria
s. pyogenes
characterized by fever, inflammation of heart, joints and BV
rheumatic fever
species which exhibits partial hemolysis
s. pneumoniae
enzyme which is oxygen stable
streptolysin S
appearance of grp A strep in culture
small, translucent and smooth
principal virulence factor for grp A strep
m-protein
painful reddish patches that enlarge and thicken with a sharply defined edge
erysipelas
species with beta, alpha and gamma rxn
enterococci
viridans streptococci
what should u do if there is a mixture of hemolytic patterns (mixed colonies)
subculture
enzyme responsible for subsurface hemolysis on BAP incubated anaerobically
strptolysin O
formerly known as erythrogenic toxins
pyrogenic toxins
upper resp tract infection which is caused by the grp A strep?
used for diagnosis?
tonsilitis/pharyngitis
throat swab
punctate exathem overlying diffuse erythema
scarlet fever
susceptibility test for scarlet fever
dick’s test
degrades proteins; mediates rash in scarlet fever
exotoxin B
enzyme responsible for surface hemolysis on BAP incubated aerobically
streptolysin S
cardinal signs of scarlet fever
diffused red rash
strawberry-colored tongue
grp of streptococci which is nosocomially transmitted
grp B strep
diagnostic test for scarlet fever?
result?
schultz charlton (+): blanching phenomenon
specimen used for lab test in grp A strep
throat swab
other name for acute glomerulonephritis
bright’s disease
streptoccoci is belong to what family
streptococcaceae
main source of streptokinase
grp C strep
differentiate s.pyogenes to other beta-hemolytic grp?
how many units?
bacitracin disc test/Taxo A
0.04 units
diffused and spreading infection characterized by redness and accumulation of fluid
cellulitis
+ result for bacitracin
susceptible
more specific than bacitracin test?
L-pyrlrolidonyl-a-naphthylamide (pyr) test
2 complications for s. pyogenes
rheumatic fever
acute glomerulonephritis
group which grows at 10C, 37C and 45C
enterococcus group
grp g strep is ____ to bacitracin
resistant/suscpetible
virulence factor of grp B strep
capsule (sialic acid)
2 notorious pathogens of streptococci
s. pyogenes
s. pbeumoniae
appearance of grp b in cultures
grayish white
part of normal flora of female genital tract and lower GIT
grp B strep
rgt used for CAMP test
B-lysin disk
strep which possesses the enzyme hippuricase
s. agalactiae
used to differentiate s. agalactiae to others
hippurate hydrolysis test
+ result for hippurate hydrolysis test?
what rgt is added?
purple color after adding ninhydrin rgt
species of grp C/G
s. dysagalactiae subsp. equisimilus
s. equi subsp. zooepdemicus
most common cause of SBE
viridans strep
(+) rsult for pyr test
bright/cherry red
+ result for CAMP test
arrow head B-hemolysis near s. aureus
bowtie appearance
specimens used for diagnosis for lancefield gros of strep
throat swab
pus secretions
sputum
blood
grp c is ____ to bacitracin and sxt
susceptible
(4) virulence factors of viridans strep
capsule
cytolysin
extracellular dextran
adhesins
most commonly isolated viridans strep
s. mutans
possesses grp D antigen
s. bovis grp
enterococci
often isolated in blood culture with GI carcinoma
bovis grp (s. equinus)
culture media used for enterococci
tsb/bhi with 5% sheep’s blood
grp of strep which are animal pathogens
grp c & g
CAMP means
Christie, Atkinson, Munch, Petersen test
formerly known as grp D strep
enterococci
(+) to 6.5% nacl?
result?
entrococci
turbid
most common isolate of enterococci
e. faecalis
specimens for diagnosing enterococci
blood
urine
wound secretions
has a characteristic “sweet odor of honeysuckle/butterscotch”
s. anginosus grp
mostly significant in classifying and identifying B-hemolytic strep
lancefield classification
to create anaerobiosis (streptolysin O)
stab (using inoculating needle) the agar
detects recent infection with s. pyogenes
anti-streptolysin O test
broth used to detect genital carriage of grp B strep during pregnancy
todd hewitt broth w/ 10 ug/mL of colistin
culture which is utilized for vaginal or rectal swabs from pregnant women
carrot broth/granada agar
enterococci: gram stain rxn
gram + cocci in pairs and long chains
used to suppress vaginal flora
15 ug/mL of nalidixic acid
all pregnant women be screened for grp B strep at?
35-37 wks of gestation
SBE means
subacute bacterial endocarditis
main source of streptokinase
grp C strep
oropharyngeal commensals
viridans streptococci
e.faecalis requires ___ for growth
B12
enterococci grow in 3 extreme conditions
alkaline pH
grow at 45 C
salt solutions
flesh-eating syndrome
necrotizing fasciitis
only beta-hemolytic strep (+) to pyr test?
rgt added?
s. pyogenes
0. 01% p-dimethyl-aminocinnamaldehyde
flesh-eating syndrome
necrotizing fasciitis
only beta-hemolytic strep (+) to pyr test?
rgt added?
s. pyogenes
0. 01% p-dimethyl-aminocinnamaldehyde
(+) rsult for pyr test
bright/cherry red
which is part of normal flora, grp A or B strep
grp b strep
all pregnant women be screened for grp B strep at?
35-37 wks of gestation
(+) rsult for camp test
bowtie appearance
animal pathogens
grp C strep
main source of streptokinase
grp C strep
also known as alpha prime strep
viridans strep
(+) result for bile esculin
black color
natural inhabitants of intestinal tracts
enterococci
beta-hemolytic grp b strep produces ____ in carrot broth after __ hrs of incubation
orange/red pigment
6
(+) to 6.5% nacl?
result?
entrococci
turbid
e.faecalis requires ___ for growth
B12
beta-hemolytic grp b strep produces ____ in carrot broth after __ hrs of incubation
orange/red pigment
6
which is part of normal flora, grp A or B strep
grp b strep
(+) rsult for camp test
bowtie appearance
animal pathogens
grp C strep
main source of streptokinase
grp C strep
also known as alpha prime strep
viridans strep
(+) result for bile esculin
black color
natural inhabitants of intestinal tracts
enterococci
m-protein has 4 characteristics
type specific
adheres to mucosal cell wall
attaches to peptidoglycan
antiphagocytic