aerobic g+ bacilli Flashcards
normal flora of human nasopharynx
c.pseudodiphtheriticum
responsible for the s/s of anthrax
protein exotoxins
acute, contagious disease characterized by systemic toxin and false membrane lining of mucus membrne of throat which leads to respiratory obstruction
diphtheria
growth factor of coryne
8 amino acids
biggest pathogen
b.anthracis
b.anthracis is part of normal flora, T/F?
motile or nonmotile?
__% NaCl is needed
F
nonmotile
7%
appearance of b.anthracis on culture (3)
medusa head colonies
beaten egg white appearance
non hemolytic
b.anthracis grows in?
produces?
ferments?
low pH (
virulence factor of b.anthracis (2)
d-glutamic capsule (antiphagocytic)
protein exotoxins
combination of these protein exotoxins may lead to death
protective antigen
lethal factor
combination of these protein exotoxins may lead to edema
edema factor
protective antigen
other name of pulmonary anthrax
woolsorter’s disease
3 colonial type of ctba, bap
mitis
intermedius
gravis
growth factor of b.anthracis
thiamine (B1)
MOT of b.anthracis (2)
inhalation
traumatic introduction
3 types pf anthrax
cutaneous
pulmonary
gastrointestinal
appearance of cutaneous anthrax
black eschar appearance
closely related to nocardia and mycobacteria
corynebacterium
fried rice bacillus
bacillus cereus
most commonly encountered bacillus in oppurtunistic inections
b.cereus
weapon for mass destruction
b.anthracis
virulence factor of b.cereus
enterotoxins (heat stable & heat labile)
which type is assocuated with ingestion of contaminated meat, vegetables, poultry?
incubation period?
symptoms?
virulence factor present?
diarrheal type
long incubation (8-16 hrs)
diarrhea
(+) heat labile enterotoxin
which type is associated with ingestion of improper stored fried rice/reheated rice?
incubation period?
symptoms?
virulence factor present?
emetic type
short incubation (1-6 hrs)
vomiting
(+) heat stable enterotoxin
common laboratory contaminant
bacillus subtilis
result when virulent strains repeatedly subcultured in vitro
avirulent
sporeforming, rod shaped organisms
bacillus
appearance of b.anthracis in microscope
bamboo fishing rod appearance
unstained central spore
susceptible to penicillin?
appearance?
b.anthracis
string of pearl appearance
virulence factor of. c.diphtheriae
diphtheriae toxin
source of bacitacin antibiotic
b.subtilis
appearance of b.subtilis in culture?
ferments? (3)
ground glass appearance
mannitol, xylose, arabinose
specimens used for lab diagnosis of b.subtilis (3)
malignant pustule
sputum
blood
specimens used for coryne
throat, nasopharyngeal swabs
used to observe the capsule of b.anthracis in blood or csf?
india ink
coryne are glucose and maltose fermenters except (2)
c. urealyticum
c. psedodiphtheriticum
2 types of food poisoning caused by b.cereus
diarrheal
emetic
PLET means
polymyxin
lysozyme
EDTA
thallous acetate
enrichment and selective tchnique
heat/alcohol shock technique before plating on media
medium used for isolation of bacillus spp from contamintaed specimen
PEA
flagella of bacilli
peritrichous
give the characteristics of coryne (3)
nonmotile
non sporeforming
non encapsulated
pleomorphic rods
animal, plant and human pathogens
corynebacterium
apperance of b.cereus in BAP
frosted glass colonies
beta-hemolytsis
feathery
c.diphtheriae is +/- to urease?
(-)
medium which induces the capsule formation of b.anthracis
bicarbonate agar
appearance of b.anthracis in gelatin medium?
(+) result?
inverted pine tree
liquefaction of b.anthracis
agar used with an appearance of opaque zone (lecithinase formation) of b.anthracis
egg yolk agar
most frequently isolated coryne
c.urealyticum
hay bacillus
b.subtilis
most clinically significant soecies of bacillus
bacillus cereus grp
biochemical test: bacillus
catalase +/-?
ferments?
hydrolyzes?
+
glucose
starch
other name of c.diphtheriae
kleb loeffler’s bacillus
appearance fo coryne in microscope
club shape bacteria (slightly curved, rod, silightly wider ends, unparallel sides
means red disease
rhusiopathiae
diphtheria can cause (2)
appearance?
tissue necrosis, exudates formation
gray to white pseudomembranous formation
c.diphtheriae can be killed by (2)
heat
disinfectants
cell wall of coryne contains ___ which is composed of? (2)
mesodiaminopimelic acid/ m-DAP
diamino acid, short chain mycolic acid
diphtheria toxin are nontoxic, unless exposed to?
process is called?
trypsin
trypsinazation
characterized by slow healing ulcers and membrane formation
cutaneous form
c.diphtheriae grp (3)
c. diphtheriae
c. ulcerans
c. pseudotuberculosis
means red skin, thread
erysipelothrix
cells are arranged in chinese letters, palisades in pairs
coryne
culture media used for coryne
CTBA
tinsdale agar
loeffler serum agar
ctba and tellurite media should be incubated for how long?
48 hrs in ambient air
used to enhance recovery form clinical soecimens (placental tissues)
what temperature?
cold enrichment technique
4 C
addition of ___ inhibits toxin production of coryne very quickly
iron
swab used in collecting the specimen for coryne
calcium alginate swab
prefrered medium for coryne
CTBA
disease affecting farm animals by feeding on plants contaminated with the spores
anthrax
only catalase negative that produces h2s
erysipelothrix rhusiopathiae
test which detects anthrax antigen
rgt used?
to observe?
ascoli test
rabbit antiserum
precipitin formation
culture media used for bacilli (5)
BAP CAP PEA PLET nutrient broth
respiratory form of diphtheria can cause (2)
pharyngitis
death
skin test for bacillus spp
ascoli test
urinary pathogen
c.urealyticum
skin test for c.diphtheriae
schick’s test
tinsdale agar is composed of (5)
(+) result
sheep's blood bovine serum cystine potassium tellurite sodium thiosulfate (+) black colonies with brown halo
forms of diphtheria (2)
respiratory form
cutaneous form
agar used for oberving morphology and metachromatic granules?
(+) result?
metachromatic granules w/in the cells?
loeffler’s serum agar
poached egg colonies
babes-ernst granule
serious infection primrly of neonates, preggy and immunocomprmsd hosts
listeriosis
to observe urease production of c.urealyticum
christensen urea slant
immunodiffusion test?
(+) result?
elek plate test
4-5 mm fine precipitin lines at 45 degree angle to the streaks
it can grow in high salt concn (10% NaCl)
listeria monocytogenes
medium used for selection and isolation of bacillus spp from contamintaed specimen
PLET
appearance of erysipelothrix rhusiopathiae in gelatin stab culture
pipe cleaner/test tube brush pattern
in vitro test?
in vivo test?
elek plate test
guinea pig lethal test
hoffman’s bacillus
c.pseudodiphtheriticum
c.ulcerans (+) result?
gelatin formation
virulence factor of listeria monocytogenes
listeriolysin O
rapid urease producer
c.urealyticum
culture media used for listeria monocytogenes (2)
mcbride agar
nalidixic acid medium
listeria monocytogenes (+) result in CAMP
block type hemolysis
CTBA means
cystine tellurite blood agar
specimen for testing b.cereus
suspected food (>= 10 to the fifth cells/g)
bacillus in microscope
boxcar-shaped
unstained spores are clear
(+) result for motility test in listeria monocytogenes
tumbling motility
umbrella-shape/inverted xmas tree
immunocompromised and pregnant women shoul avoid eating?
soft cheese
b.cereus ferments?
salicin
c.pseudodiphtheriticum (+) result
nitrate production
common cause of diphtheroid prosthetic valve endocarditis
c.jeikeium
appearance of listeria monocytogenes in BAP
translucent grayish-blue colonies
coryne which produces dermonecrotic toxin
c.pseudotuberculosis
bacillus cereus grp (4)
b. cereus
c. anthracis
b. thuringiensis
b. mycoides
(+) h2s production?
what medium?
erysipelothrix rhusiopathiae
triple sugar iron medium
c.diphtheriae on gram stain
highly pleomorphic g+ short or slightly curved rods with rounded ends