staph & strep (lab) Flashcards
most commonly isolated viridans strep
s.mutans
test which detects unbound coagulase?
(+) result within?
screening?
confirmation?
tube method
clot formation within 4 hrs
1-4 hrs
20 hrs
culture: BAP staph
buttery looking/ creamy,white or light gold
staph 2 coagulase (-) species
s. epidermidis
s. saprophyticus
bacitacin test or?
units?
to differentiate?
which is susceptible/resistant?
taxo A disk tes
0.04 units
micrococci (s; >10mm zone of inhibition) & staph (r)
differentiates patho and nonpatho staph?
(+) result? in what organism?
agar used?
composed of?
mannitol fermentation test
yellow; s.aureus
MSA
1%mannitol and 7.5% NaCl
most common isolated enterococci
e.faecalis
test which detects dnase activity of s.aureus?
medium and rgt used?
(+) result?
DNAse test
DNA medium; methyl green dye
clearing of dye
rgt for coagulase test
edta plasma
staph
normal flora of skin?
cause what diseases? (2)
s.epidermidis
endocarditis, prostethic 💟 valve
test for identification of s. saprophyticus?
zone of inhibition?
5 ug novobiocin: resistant
6-12 mm
grp which is only susceptible to bacitracin
A
s.epidermidis is ___ to novobiocin?
zone of inhibition?
susceptible
16-27mm
coagulase (-) staph by tube method
s.lugdunensis
characteristics of strep (5)
catalase (-) fastidious pairs, chains facultative anaerobe capnophilic
alpha hemolytic strep
s.pneumoniae
appearance in microscope (staph)
spherical in clusters
beta hemolytic strep (4)
s. pyogenes
s. agalactiae
s. dysagalactiae, s.equi
SXT means
sulfamethoxazole trimethoprim
diseases caused by s.pnuemoniae
meningitis
lobar pneumonia
otitis media
staph
cause of UTI in women?
urine culture?
s. saprophyticus
10,000 CFU/mL
lab test results for viridans strep
bile insoluble
optochin resistant
test for identification of agalactiae?
rgt?
(+) result?
CAMP test
beta-lysin disk
bowtie appearance
CAMP means
christie
atkinson
munch
petersen
rgt for hippurate hydrolysis test?
(+) result?
Na hippurate + ninhydrin
purple
rgt for catalase test
3% h2o2
most virulent staph
s.aureus
presumptive test for s.pneumoniae?
rgt?
result?
optochin susceptibility/p disk test
ethyhydroxycuprein hcl
>= 14 mm
enterococcus (3)
e. faecalis
e. faecium
e. durans
(+) result for neufeld quellung
swelling of capsule
tests which differentiate grp D and grp E enterococci?
tests which E is (-)
bile esculin, 6.5% NaCl, PYR
nacl, pyr
gro C,F,G is ____ to bacitracin
resistant
grp which is only susceptible to SXT
C
rgt used for modified oxidase test?
(+) result?
1% tetramethyl-p-phenylenediamene dihydrochloride
purple
test for identification of s.pyogenes?
result?
bacitracin
susceptible
test which detects cell bound coagulase?
(+) result? within?
slide method
clot formation within 30 sec
normal flora of URT, GIT, GUT
viridans strep
confirmatory test for s.pneumoniae?
rgt?
incubate for how long? with tempt
(+) result?
bile solubility
Na desoxycholate
3 hrs at 35 C
clear
non enterococcus
s. bovis
s. equinis
more specific test than bacitracin for pyogenes?
rgt?
(+) result?
L-pyrrolidonyl-alpha-naphthylamide test
0.01% p-dimethyl-aminocinnamaldehyde
cherry red
known as nutritionally variant strep?
appearance in culture?
growth factors?
abiotrophia and granulicatella
satellites
thiol compounds (cystein,pyrodoxal,vitb6)
not classified under lancefield?
species?
viridans strep
s.mitis,mutans,sanguis
give 5 charcteristics of staph
catalase-producing bacteria facultative anaerobe non motile non spore forming glucose fermenters
gamma hemolytic strep
e. faecalis
e. facium
