entero-gram negative Flashcards
serological test identifies grp A strep through
latex agglutination/nucleic acid probe
rgt for catalase test
rxn to enterecocci
3% hydrogen peroxide
(-) no bubble formation
LAP means
Leucine aminopeptidase test
antigen detection
serological test
drug of choice for penicillin-resistant n.gonorrhea
ceftriaxone
differentiates aerococcus and leuonostoc to other g+ cocci
lap test
appearance of moraxella catarrhalis in culture
hockey puck appearance
young colonies of s.pneumoniae
dome-shaped
glistening
wet
mucoid
uses antibody bound to a particle to enhance visibility of agglutination rxn between Ag and Ab
co-agglutination test
modt common isolate inchildren with recurrent otitis media
s. pneumoniae
test which detects acetoin production from glucose
voges-proskauer test
virulence factor of s. pnuemoniae
capsular polysaccharide
present in martin lewis medium
all in modified TMA except nystatin, which is replaved by anisomycin: yeast
(+) for voges proskauer test
end product ?
anginosus grp
acetoin (red/pink color)
appear as satellites around an organism that produces pyridoxal
abiotrophia and granulicatella
s. pnemoniae in microscope
gram-positive cocci in pairs, oval or lancet shape, faculatatively anaerobe
(-) to LAP test
aerococci
leuconostoc
specimens used in lab diagnosis for s. pneumonie
sputum
blood
csf
growth factors of abiotrophia and granulicatella
thiol compounds (cystein,vit b6,pyridoxal)
most commonly isolated member of genus moraxella
moraxella catarrhalis
old colonies of s.pneumoniae
coin w/ raised rim
dimple/donut-shaped
gram stain: s. pneumoniae
direct smearsof csf reveal wbcsand gram+ cocci in pairs
3 differential tests for s. pneumoniae
optochin susceptibility/p disk test
bile solubility
neufeld quellung rxn
(neufeld quellung rxn)
this is mixed with thesputum,csf,etc along with methylen blue
antipneumococcal serum
opsonized particles are more easily phagocytosed dueto presence of ___ on plasma membrane
complement receptors
*s.pneumoniae presumptive test? rgt used? susceptibility medium? result?
optochin susceptibility/p disk test
ethylhydroxycuprein hydrochloride (6 or 10 um)
BAP
greater or equal to 14 mm
differentiates pneumococcus from viridans strep
bile solubility
compliction of pneumonia and otitis meia
meningitis
(+) result of m.catarrhalis in butyrate esterase test
blue color
(+)result of neufeld quellung rxn
swellingof capsule
formerly known as nutritionally variant streptococci
abiotrophia
granulicatella
appearance of neisseria in culture
small, gray-white opaque,convex, glistening colonies
capsule of s.pneumoniae is
antigenic
abiotrophia and granulicatella are(3)
pyridoxal/vit b6-dependent
thiol-dependent
symbiotic streptococci
opsonization of the capsule of s.pneumoniae renders the organism
avirulent
3rd most common cause of otitis media and sinusitis
moraxella catarrhalis
give at least 7 characteristics of neisseria
obligate aerobic non motile non hemolytic capnophiles (2-8%) sensitive to drying and extreme heat carbohydrate fermenters fastidious; enriched medium is used
bestmethod for culture andtransport of n.gonorrhea is
inoculate the agar immediately and place the medium in an increased CO2 such as coplin jar, JEMBEC system
culture media for meningitidis (3)
bap
cap
tma
neisseria observed in microscope
rod shaped?
gram-negative diplococci with coffee or kidney bean shaped
N. elongata, N. weaver
only member of family neisseriaceae which is (+) on DNAse test
m. catarrhalis
natural habitat of neisseria
mucuos membrane of respiratory & urogenital tract
n.menigitidis is sensitive to ___; this should not exceed ___
sps; 0.025%
beta lactamase test?
rgt?
(+) result?
cephalosporinase test
cephalosporin disk
deep pink
gram variable and pleomorphic in microscope
abiotrophia
granulicatella
*s.pneumoniae
confirmatory test?
rgt used?
result?
bile solubility
sodium desoxycholate(bile salt)
(+) bile soluble, (-) turbid
biochemical identification of n.gonorrhea
(+) result?
coagglutination
agglutination
major pathogens of neisseria (2)
n. gonorrhea
n. meningitidis
presence of n.menigitidis in blood
meningococcemia
infection shows bloody, rust stinged sputum
lobar pneumonitis
other name for n. gonorrhea
only known host
gonococci
man
n. gonorrhea requires __ for growth
it produces ___ which is important for pathogenesis
virulence factor?(for adherence)
iron
igA protease
common pili
colonial typesof n.gonorrhea:
virulent?
avirulent?
t1 t2
t3 t5
present in new york city medium
vancomycin
colistin
trimethoprim
amphotericin B: yeast
in n.gonorrhea, these rare isolates are isolated from asymptomatic men
auxotypes
(n.meningitidis)
clsi recommends what broth? and what agar microdilution?
with?
MH broth with 2-5% laked horse blood
MHA with 5% sheep blood
AHU stains (3)
arginine
hypoxanthine
uracil
means “flow of seed” and “brothel”
gonorrhea
endotoxin produces hemorrhage in adrenals, which causes what syndrome
waterhouse-friderichsen syndrome
other name for conjuctivitis
opthalmia neonatorum
chief complaint oropharyngeal infection
pharyngitis
specimens used for n.gonorrhea
pus and secretions from cervix,urethra, throat,prostate
specimen collection for n.gonorrhea
then place inwhat transport medium? how long?
dacron or rayon swabs
amies medium for 6 hrs
screening test for n.gonorrhea
nucleic acid amplification
JEMBEC means
james e. martin biological environmental chamber
confirmation of n.gonorrhea is based on (2)
growth and biochemical characteristics
uses monoclonal bodies that recognizes epitope;
also confirms morphologic appearance
fluorescent antibody test/FAT
antimicrobial agents present in modified TMA, suppressed organism
all in TMA
trimethoprim plate: swarming of proteus
pyridoxal producers (4)
e.coli
kliebsiella spp
enterobacter spp
yeast
virulence factors of n.meningitidis (4)
common pili
capsular polysaccharide
endotoxin
igA1
confirmatory test for n.gonorrhea
chemiluminescent nucleic acid probe
present in GC-LECT medium
all in NYC medium
lincomycin: g+
other bacteria with (+) growth on gonococcal media (3)
acinetobacter
capnocytophaga
k.denitrificans
standard method in identifying n.gonorrhea
detects?
medium?
(+) result?
CHO utilization test
acid production from glucose, maltose, lactose, fructose
cystine trypticase agar
yellow color at 35-37C
rgt used for oxidase test in neisseria
(+) result?
1% tetramethyl-p-phenylenediamine dihydrochloride
purple color within 10 sec
s. pnuemoniae is also known as
diplococcus/pneumococcus
culture media for n.gonorrhea
if swabs, what pattern?
bap & cap
z-pattern and cross streaked with loop
antimicrobial agents present in TMA (3); and their suppressed organism
vancomycin: g+
colistin: g-
nystatin: yeast
staph and strep are motile, T/F
F, brownian movement
untreated gonococcal cerviitis may cause
fitz-hugh-curtis synrome
(+) to LAP test?
end product?
viridans streptococci
stretococcus
enterococcus
pediococcus
B-naphthylamine (red color)
