Sterilization and Storage Flashcards
What is sterilisation?
Sterilisation is the process in which all living microorganisms including spores are killed
What is sterilisation assurance level?
Device is free from microorganisms defining on probability that the viable microbes on the instrument is less than or equal to 1 in a million
Name 3 general methods of sterilization
- Physical
- Chemical
- Physio-Chemical
Name 2 types of chemical sterilization
- Liquids (alcohols, halogens)
2. Gaseous (Formaldehyde, Ethylene oxide)
Name 5 types of physical sterilization
- Sunlight
- Heat (dry or moist)
- Vibration
- Radiation (ionizing or non-ionizing)
- Filtration (asbestos, membrane)
Describe how sterilization is generally carried out in dentistry
- Moist heat in autoclave
- Saturated steam under pressure
- 98% steam or 2% water
Name 5 advantages of steam sterilization
1, Rapid heating and penetration of load
- Rapid destruction of microorganisms
- No toxic residues
- Low cost process
- Easily controlled and monitored
Name 5 phases of steam sterilization
- Heating
- Air removal
- Sterilisation
- Evacuation and cooling
- Drying
Name 4 factors which affect sterilization
- Amount of organic material
- Number of microorganisms
- Type of microorganisms
- Steam quality
Name 5 instrument factors which affect sterilisation
- Rough pitted surfaces
- Lumens
- Hinges
- Heat sensitive devices
- Over packing
Name 3 methods of air removal in autoclave
- Downward gravity displacement
- Vacuum removal
- Pulse
What is Type N Bench-Top Sterilizer?
Non-vacuum sterilisers for non-wrapped solid instruments where air removal is passive displacement by steam
What is Type B Bench-Top Sterilizer?
Vacuum air actively removed reprocess load trays such as hollow, air retentive packaged loads
What is Type S Bench-Top Sterilizer?
Vacuum or pulse system, specifically designed to reprocess specific load types
Describe 3 parameters of the sterilisation phase
- Temperature at 134 - 137 C
- Pressure at 2.0 - 2.3 bar
- Holding time of 3 minutes