Sterilization and Disinfection

Question 1

Removal or Destruction of all forms of life

a. Sterilization
b. Disinfection

Answer 1

a. Sterilization

disinfection - removal, INHIBITION OR KILLING OF MICROORGANISMS, although it DOES NOT REMOVE ALL BACTERIAL SPORES

Question 2

is the Most Commonly Used Method for removal of microorganisms

a. heat
b. Filtration
c. radiation

Answer 2

a. heat

Question 3

FASTEST AND SIMPLEST method of sterilization, killed within 15 mins

a. boiling
b. autoclaving
c. inspissation

Answer 3

b. autoclaving

Question 4

it destroy vegetative bacteria (non-spolurating)
100*c for 10-15mins

a. boiling
b. autoclaving
c. inspissation

Answer 4

a. boiling

Question 5

used to sterilize BIOHAZARDOUS TRASH AND HEAT STABLE OBJECTS

A. autoclaving

b. boiling
c. fractional

Answer 5

a. autoclaving

Question 6

used is steam under pressure

a. autoclaving
b. fractional
c. pasteurization

Answer 6

a. autoclaving

Question 7

the instrument used in FRACTIONAL OR INTERMITTENT STERILLIZATION

a. Arnold’s sterilizer
b. Anton sterilizer
c. aton sterilizer

Answer 7

a. Arnold sterilizer

Question 8

performed at 100*C for 30 mins for 3 consecutive days

a. fractionation/ intermittent sterilization
b. inspissation

Answer 8

a, fractionation or intermittent sterilization

Question 9

performed at 70C to 80C for 2 hours for 3 consecutive days

a. fractionation/ intermittent sterilization
b. inspissation

Answer 9

b. inspissation

Question 10

used to sterilize PROTEIN RICE MEDIUM (LJ MEDIUM)

a. boiling
b. inspissation
b. autoclaving

Answer 10

b. inspissation

Question 11

destroys vegetative cells and spore for 3 consecutive days of sterilization

Answer 11

fractionation or intermittent sterilization

Question 12

it is used to sterilize MILK, DAIRY PRODUCTS AND ALCOHOLIC BEVERAGES

Answer 12

pasteurization

Question 13

quick heating then immediate cooling ( 72*C for 15 seconds)

a. low temperature holding/ batch method
b. high temperature short time (HTST)/ flash pasteurization
c. ultra high temperature (UHT)

Answer 13

b. high temperature short time (HTST)/ flash pasteurization

Question 14

cooled very quickly in a vacuum chamber (140*C for 3 secs)

a. low temperature holding/ batch method
b. high temperature short time (HTST)/ flash pasteurization
c. ultra high temperature (UHT)

Answer 14

c. ultra high temperature (UHT)

Question 15

sterilization method that DOES NOT REQUIRE WATER

a. moist heat procedure
b. dry heat procedure

Answer 15

b. dry heat procedure

Question 16

utilized for sterilization of glassware, oil products and powder

a. moist heat
b. dry heat
c. filtaration

Answer 16

b. dry heat

Question 17

biological indicator of dry heat procedure?

a. Bacillus atrophaeus
b. Bacillus stearothermophilus
c. Bacillus subtilis var. niger

Answer 17

a&c Bacillus atrophaeus ( Bacillus subtilis var. niger)

Question 18

bilogical inidcator of autoclaving

a. Bacillus atrophaeus
b. Bacillus stearothermophilus

Answer 18

b. Bacillus stearothermophilus / Geobacillus stearothermophilus

Question 19

most common method of treating infectious waste and infected laboratory animals

a. oven heating
b. flaming
c. incineration

Answer 19

c. incineration

Question 20

what is the temperature used in HAZARDOUS MATERIAL?

a. 870C to 980C
b. 300 to 400C
c. 160 to 170C

Answer 20

a. 870C to 980C

Question 21

used to control the spread of communicable disease

a. flaming
b. oven heating
c. incineration
d. cremation

Answer 21

cremation

Question 22

it is the method of choice for STERILIZATION OF ANTIBIOTIC SOLUTIONS, TOXIC CHEMICALS, RADIOISOTOPES, VACCINES AND CARBOHYDRATES

a. sterilization
b. disinfectants
c. filtration

Answer 22

c. filtration

Question 23

it is a type of filter that is made up FIBROUS OR GRANULAR MATERIALS

a. depth filter
b. hepa filter
c. membrane filter

Answer 23

a. depth filter

Question 24

This type of filter is used to sterilize PHARMACEUTICALS, OPTHALMIC SOLNS, CULTURE MEDIA, ANTIBIOTICS AND OIL PRODUCTS

a. depth filter
b. hepa filter
c. membrane filter (circular filter)

Answer 24

c. membrane filter (circular filter)

Question 25

it is imporatant in FOOD MICROBIOLOGY

a. hepa filter
b. osmotic pressure
c. low/cold temperature

Answer 25

c. low/cold temperature

Question 26

it destroys bacteria through the disruption of metabolism that involves removing water from microbes

a. dessication
b. lyophilization

Answer 26

a. dessication

Lyophilization - destroy bacteria through CHANGES IN PROTEINS AND DECREASE IN CHRMICAL REACTIONS

Question 27

the plasma membrane shrinks away from the cell wall (cell may not die but usually stop growing) as water leaves the cell

Answer 27

plasmolysis

Question 28

it use of high concentration of salts and sugar in food to create a hypertonic enviroment

a. osmotic pressure
b. cold/ low temperature
c. lyophilization

Answer 28

a. osmotic pressure

Question 29

biological indicator of RADIATION

a. bacillus niger
b. bacillus pumilus
c. bacillus steathermophilus

Answer 29

b. bacillus pumilus

Question 30

it refers to the removal, INHIBITION OR KILLING OF MICROORGANISMS, although it DOES NOT REMOVE ALL BACTERIAL SPORES

a. radiation
b. disinfection
c. sterilization

Answer 30

b. disinfection

Question 31

ratio of Sodium hypochlorite (NaOCL) to be effective disinfectant

a. 10:1
b. 1:10
c. 1:20

Answer 31

b. 1:10

Question 32

it participates bacterial protein and kills all bacteria in the specimen

a. bactericidal
b. bacteriostatic
c. disinfectant

Answer 32

a. Bactericidal

bacteriostatic - inhibit the GROWTH OF ORGANISMS

disinfectant- applied to inanimate objects

Question 33

first widely used antiseptic and disinfectant

a. alcohol
b. aldehyde
c. formaldehyde
d. phenol

Answer 33

d. Phenol (Tuberculoidal)

Question 34

chemical sterilant for HEPA filters in a biological safety cabinet

a. phenol
b. formaldehyde
c. halogens

Answer 34

b. formaldehyde

Question 35

most commonly used gas in sterilization

a. carbon dioxide
b. etyhylene oxide (EtO)
c. both

Answer 35

b. ethylene oxide