Bacterial Staining Flashcards

1
Q

What are the different TECNIQUES in staining

A
  1. Simple staining
  2. Differential staining
  3. Negative staining
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2
Q

It colors the FORMS AND SHAPES of the cells

a. simple staining
b. differential staining
c. negative staining

A

a. simple staining

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3
Q

it demonstrate the presence of DIFFUSE CAPSULE surrounding the bacteria

a. simple staining
b. differential staining
c. negative staining

A

c. negative staining

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4
Q

excellent technique in studying thE BACTERIAL GAS VACUOLES AND VIRAL MORPHOLOGY

a. simple staining
b. differential staining
c. negative staining

A

C. NEGATICE STAINING

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5
Q

IT is the most commonly used DIFFERENTIAL stain in clinical microbiology laboratory

A

GRAM STAIN

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6
Q

it is the SECONDARY STAIN/ COUNTERSTAIN in gram stain

a. crystal violet
b. safranin
c. iodine

A

safranin

** crystal violet = primary stain
iodine = mordant

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7
Q

it contain LIPOPOLYSACCHARIDES

A. GRAM POS
B. GRAM NEG

A

GRAM NEG

GRAM POS contains TEICHOIC ACID

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8
Q

ALL COCCI are gram pos except for?

A
  1. Neisseria
  2. Veilonella
  3. Branhamella (Moraxella)
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9
Q

Organisms that EXIST MOST EXCLUSIVELY WITHIN the host cells

a. Mycoplasma
b. Chlamydia
c. Ureaplasma

A

Chlamydia

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10
Q

organisms with INSUFFICIENT DIMENSION TO RESOLVED BY LIGHT MICROSCOPY

A. MYCOPLASMA AND UREAPLASMA
B. CHLAMYDIA
C. SPIIROCHAETES

A

C. Spirochaetes

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11
Q

it is the method of acid fast stain that is IDEAL FOR CONCENTRATED SMEARS AND PARTIALLY ACID FAST bacilli like Nocardia spp

a. Ziehl Neelsen
b. Kinyoun
c. Papenheimer

A

a. Ziehl - Neelsen method

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12
Q

it is composed of HYDROCHLORIC ACID AND ETHANOL

A

acid alcohol

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13
Q

it is used to stain bacteria with HIGH LIPID CONTENTS in their cell wall

A

acid fast stain

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14
Q

acid staining methods that differentiates Mycoplasma leprae from MTB

a. Pappenheim method
b. auramine rhodamine
c. baumgarten method

A

c. baumagarten method

papenheim method = diff mycoplasma smegmatis from mtb

auramine rhodamine = selective for the cell wall of acid fast

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15
Q

it is useful for identification of INTETSINAL COCCIDIAN OOCYST

a. mod. kinyoun
b. ziehl neelsen
c. gram stain

A

mod. kinyoun or mod acid fast staining md

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16
Q

it is ideal for cryptosporidia and cyclospora parasites

a. mod. kinyoun
b. ziehl neelsen
c. gram stain

A

a. mod kinyoun

17
Q

which pf the ff methods used for DNA staining

a. dyar stain
b. feulgel stain
c. india ink

A

b. fuelgel stain