Bacterial Staining Flashcards
What are the different TECNIQUES in staining
- Simple staining
- Differential staining
- Negative staining
It colors the FORMS AND SHAPES of the cells
a. simple staining
b. differential staining
c. negative staining
a. simple staining
it demonstrate the presence of DIFFUSE CAPSULE surrounding the bacteria
a. simple staining
b. differential staining
c. negative staining
c. negative staining
excellent technique in studying thE BACTERIAL GAS VACUOLES AND VIRAL MORPHOLOGY
a. simple staining
b. differential staining
c. negative staining
C. NEGATICE STAINING
IT is the most commonly used DIFFERENTIAL stain in clinical microbiology laboratory
GRAM STAIN
it is the SECONDARY STAIN/ COUNTERSTAIN in gram stain
a. crystal violet
b. safranin
c. iodine
safranin
** crystal violet = primary stain
iodine = mordant
it contain LIPOPOLYSACCHARIDES
A. GRAM POS
B. GRAM NEG
GRAM NEG
GRAM POS contains TEICHOIC ACID
ALL COCCI are gram pos except for?
- Neisseria
- Veilonella
- Branhamella (Moraxella)
Organisms that EXIST MOST EXCLUSIVELY WITHIN the host cells
a. Mycoplasma
b. Chlamydia
c. Ureaplasma
Chlamydia
organisms with INSUFFICIENT DIMENSION TO RESOLVED BY LIGHT MICROSCOPY
A. MYCOPLASMA AND UREAPLASMA
B. CHLAMYDIA
C. SPIIROCHAETES
C. Spirochaetes
it is the method of acid fast stain that is IDEAL FOR CONCENTRATED SMEARS AND PARTIALLY ACID FAST bacilli like Nocardia spp
a. Ziehl Neelsen
b. Kinyoun
c. Papenheimer
a. Ziehl - Neelsen method
it is composed of HYDROCHLORIC ACID AND ETHANOL
acid alcohol
it is used to stain bacteria with HIGH LIPID CONTENTS in their cell wall
acid fast stain
acid staining methods that differentiates Mycoplasma leprae from MTB
a. Pappenheim method
b. auramine rhodamine
c. baumgarten method
c. baumagarten method
papenheim method = diff mycoplasma smegmatis from mtb
auramine rhodamine = selective for the cell wall of acid fast
it is useful for identification of INTETSINAL COCCIDIAN OOCYST
a. mod. kinyoun
b. ziehl neelsen
c. gram stain
mod. kinyoun or mod acid fast staining md
