Sterile and Non-Sterile study questions Flashcards
What are 2 criteria used to decide whether a specimen should be considered sterile and worked up as such or “nonsterile”
- where it is from (source)
- How was it obtained (can you get more)
Media needed for nonsterile
BAP, MAC
Media needed for sterile
BAP, MAC, CHOC
Gram stain for nonsterile
-gram stain not routinely performed
-don’t need to notify unless necrotizing fasciitis
Gram stain for sterile
-need gram stain to move forward
-notify the provider as soon as gram media is made and bacteria seen
Length of incubation for nonsterile
2 days
Length of incubation for sterile
5 days max
-Steriles are held longer
Number of isolates identified in nonsterile
-3 isolates but if you get up to 4 not much work
Number of isolates identified in sterile
-report any number of pathogens
-freeze and call the doctor
The extent of workup for nonsterile
-descriptive ID (NLF)
The extent of workup for sterile
-Full ID
Priority of the report for nonsterile
-no reason to call the doctor unless group A strep, necrotizing fascitis, GC
Priority of the report for sterile
-urgent, as soon as gram stain call doctor
Finger laceration: pathogen found there and nonpathogen
-beta heme strep, staph aureus (P)
-diphtheroid, micrococcus, peptostrepococcus (NP)
Ear drainage: pathogen and nonpathogen found there
-staph epidermidis (and skin flora) (NP)
-S. pneumonia, pseudomonas (swimmer’s ear),, Haemophilus, MCATT (P)
Eye culture: pathogen and nonpathogen found there
-corynebacterium. skin flora (NP)
-coag neg staph, gonorrhea, rothia, alpha strep, Hflu, strep pneumonia (P)
Dog bite wound: pathogen and nonpathogen found
-pasturella (P)
-skin flora (NP)
Human bite mark: pathogen and nonpathogen found
-eikinella (P)
-oral flora (NP)
diabetic foot ulcers: pathogens and nonpathogen
-Yeast**, staph aureus (P)
-skin flora (NP)
Burn site: pathogens and nonpathogens
-pseudomonas **, staph aureus, gram neg (kleb, e.coli, entero, proteus) (P)
-skin related flora (NP)
What is the impact of detecting antibiotic-resistant bacteria such as MRSA, VRE, ESBL, and CRE, from a hospital patient
-to prevent the spread to other hospital patients
-make sure the right antibiotic treatment is made
Describe the technique used for plating an IV catheter tip
-Use the MAKI technique, take your sterile get 5 ml of tip, take your BAP, and innoculate with the cup
-need more than 15 isolates to get worked up, Under 15 does not
-you are most likely to have staph epidermis (forms biofilm), corynebacterium
