Sterile and Non-Sterile study questions

Question 1

What are 2 criteria used to decide whether a specimen should be considered sterile and worked up as such or “nonsterile”

Answer 1

1. where it is from (source)
2. How was it obtained (can you get more)

Question 2

Media needed for nonsterile

Answer 2

BAP, MAC

Question 3

Media needed for sterile

Answer 3

BAP, MAC, CHOC

Question 4

Gram stain for nonsterile

Answer 4

-gram stain not routinely performed
-don’t need to notify unless necrotizing fasciitis

Question 5

Gram stain for sterile

Answer 5

-need gram stain to move forward
-notify the provider as soon as gram media is made and bacteria seen

Question 6

Length of incubation for nonsterile

Answer 6

2 days

Question 7

Length of incubation for sterile

Answer 7

5 days max
-Steriles are held longer

Question 8

Number of isolates identified in nonsterile

Answer 8

-3 isolates but if you get up to 4 not much work

Question 9

Number of isolates identified in sterile

Answer 9

-report any number of pathogens
-freeze and call the doctor

Question 10

The extent of workup for nonsterile

Answer 10

-descriptive ID (NLF)

Question 11

The extent of workup for sterile

Answer 11

-Full ID

Question 12

Priority of the report for nonsterile

Answer 12

-no reason to call the doctor unless group A strep, necrotizing fascitis, GC

Question 13

Priority of the report for sterile

Answer 13

-urgent, as soon as gram stain call doctor

Question 14

Finger laceration: pathogen found there and nonpathogen

Answer 14

-beta heme strep, staph aureus (P)
-diphtheroid, micrococcus, peptostrepococcus (NP)

Question 15

Ear drainage: pathogen and nonpathogen found there

Answer 15

-staph epidermidis (and skin flora) (NP)
-S. pneumonia, pseudomonas (swimmer’s ear),, Haemophilus, MCATT (P)

Question 16

Eye culture: pathogen and nonpathogen found there

Answer 16

-corynebacterium. skin flora (NP)
-coag neg staph, gonorrhea, rothia, alpha strep, Hflu, strep pneumonia (P)

Question 17

Dog bite wound: pathogen and nonpathogen found

Answer 17

-pasturella (P)
-skin flora (NP)

Question 18

Human bite mark: pathogen and nonpathogen found

Answer 18

-eikinella (P)
-oral flora (NP)

Question 19

diabetic foot ulcers: pathogens and nonpathogen

Answer 19

-Yeast**, staph aureus (P)
-skin flora (NP)

Question 20

Burn site: pathogens and nonpathogens

Answer 20

-pseudomonas **, staph aureus, gram neg (kleb, e.coli, entero, proteus) (P)
-skin related flora (NP)

Question 21

What is the impact of detecting antibiotic-resistant bacteria such as MRSA, VRE, ESBL, and CRE, from a hospital patient

Answer 21

-to prevent the spread to other hospital patients
-make sure the right antibiotic treatment is made

Question 22

Describe the technique used for plating an IV catheter tip

Answer 22

-Use the MAKI technique, take your sterile get 5 ml of tip, take your BAP, and innoculate with the cup
-need more than 15 isolates to get worked up, Under 15 does not
-you are most likely to have staph epidermis (forms biofilm), corynebacterium