Disinfectant and Antiseptics Flashcards
Static
-agents that inhibit the growth of a microbe
cidal
agents that kills a microorganism
bactericide
kills bacteria
bacteriostatic
prevent bacteria from reproducing
Disinfectant
an antimicrobial used to kill microorganisms on non-living surfaces or inanimate objects
-are more concentrated or can be left on surfaces longer
Antiseptic
an antimicrobial used to kill microorganisms on skin or body tissue
Alcohols
-evaporate allowing them to stay on skin long enough to kill many microbes but not long enough to cause damage to our cells
-are static to microbes because they denature essential proteins and disrupt plasma membranes
ex: ethanol, isopropanol
Phenols and Phenolic
-many household, commercial and lab disinfectants are phenolic
-lysol the most common
-they denature proteins and disrupt the plasma membrane of microbes
-they remain longer on the surface than alcohols
agar plate sensitivity method
used to determine the effectiveness of disinfectants and antiseptics
1. TSA agar plates are uniformly inoculated with bacteria species of interest
2. standard amount an antiseptic or disinfectant is added to the disk or wafer
3. zone of inhibition are observed
** zone of inhibition does not relate to the chemicals degree of effectiveness
How does hydrogen peroxide kill bacteria
-hydrogen peroxide is an oxidizing agent
Oxidizing agent
-chemical that steals or strips electrons from other chemicals
-will damage and destroy vital structures and biochemicals in microbes by stealing their electrons
fungistatic
inhibits growth of fungi
bactericidal
actively kills bacteria
sporicidal
actively will kill the spores of fungi
bacteriostatic
inhibits the growth of bacteria
