STEPS IN VIRAL REPLICATION and specimen collection

Question 1

STEPS IN VIRAL REPLICATION

Answer 1

1. Attachment/Adsorption
2. Penetration
3. Uncoating
4. Macromolecular synthesis
5. Viral assembly
6. Lysis and release

Question 2

recognition of host cell and specific binding between viral capsid proteins and the receptor of the host cell

Answer 2

Attachment/Adsorption

Question 3

Viruses enter the host cell

Answer 3

PENETRATION

Question 4

______ can penetrate the cell membrane directly.

Answer 4

Naked virions

Question 5

________ may enter the cell by fusion with the cell membrane or via _______ whereby the ______ enters in a vacuole

Answer 5

Enveloped viruses;
endocytosis;
enveloped virus

Question 6

process by which the capsid is removed

Answer 6

Uncoating

Question 7

In uncoating, _______ usually release the genome into the cytoplasm, whereas most _____ release their genome into the host nucleus

Answer 7

RNA viruses;
DNA viruses

Question 8

production of nucleic acid and protein polymers

Answer 8

Macromolecular synthesis

Question 9

process by which structural proteins, genomes, and in some cases viral enzymes are assembled into virus particles

Answer 9

Viral assembly

Question 10

new virions are then released by lysis if they are naked viruses or by budding if they are enveloped viruses

Answer 10

Lysis and release

Question 11

In lysis and release, if they are naked viruses, they are released by

Answer 11

released by lysis

Question 12

In lysis and release, if they are enveloped viruses, they are released by

Answer 12

budding

Question 13

in Specimen Collection and Transport, what swab to use

Answer 13

Use Dacron, Rayon swab

Question 14

Storage for specimen

Answer 14

4°C for 5 days;
if >6 days = 20°C or –70°C