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VIROLOGY > Laboratory Diagnosis > Flashcards

Laboratory Diagnosis Flashcards

1
Q

Most rapid method of diagnosis

A

Electron Microscopy

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2
Q

For nonculturable viruses

A

Electron Microscopy

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3
Q

Detection of cytoplasmic inclusions/cytopathic effects

A

Light Microscopy

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4
Q

Light Microscopy cytoplasmic inslusions (7)

A

a. Negri bodies
b. Torres Councilman bodies
c. Cowdry Type A
d. Owl’s eyes
e. Koilocytes
f. Henderson-Patterson bodies
g. Guarnieri bodies

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5
Q

➔ Rabies (invades nervous system)
➔ Can be observed only after the autopsy of the
dead patient

A

Negri bodies

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6
Q

Yellow fever inclusion

A

Torres Councilman bodies

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7
Q

HSV inclusion (Herpes Simplex Virus)

A

Cowdry Type A

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8
Q

VZV ( varicella-zoster virus) inclusion

A

Cowdry Type A

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9
Q

CMV (cytomegalovirus) inclusion

A

Owl’s eyes

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10
Q

HPV (Human Papillomavirus) inclusion

A

Koilocytes

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11
Q

Molluscum contagiosum inclusion

A

Henderson-Patterson bodies

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12
Q

Pox virus inclusion

A

Guarnieri bodies

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13
Q

Cytologic Smear (2)

A

a. Pap Smear
b. Tzanck Smear

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14
Q

Smear for HSV and VZV; multinucleated giant cell

A

Tzanck Smear

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15
Q

smear for detection of HPV carcinoma

A

Pap Smear

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16
Q

culture obtained from animals

A

Primary Cell Culture

17
Q

subcultivated primary culture; normal human cells

A

Diploid Cell Culture

18
Q

Primary cell culture (3)

A

● Primary Monkey Kidney
● Human Embryonic Kidney
● Rhesus Kidney

19
Q

Diploid Cell Culture (3)

A

MRC-5
WI-38
Human Diploid Fibroblast (HDF)

20
Q

immortal cell lines; cancer cells

A

Continuous Cell Culture

21
Q

Continuous Cell Culture (4)

A

HeLa
Hep2
A-549
AGMK

22
Q

Serology (3)

A

a. Neutralization
b. Complement fixation
c. Hemagglutination inhibition

23
Q

detects blockade of cytopathic effects

A

Neutralization

24
Q

for influenza and rubella

A

Hemagglutination inhibition

VIROLOGY (8 decks)

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