Laboratory Diagnosis Flashcards
Most rapid method of diagnosis
Electron Microscopy
For nonculturable viruses
Electron Microscopy
Detection of cytoplasmic inclusions/cytopathic effects
Light Microscopy
Light Microscopy cytoplasmic inslusions (7)
a. Negri bodies
b. Torres Councilman bodies
c. Cowdry Type A
d. Owl’s eyes
e. Koilocytes
f. Henderson-Patterson bodies
g. Guarnieri bodies
➔ Rabies (invades nervous system)
➔ Can be observed only after the autopsy of the
dead patient
Negri bodies
Yellow fever inclusion
Torres Councilman bodies
HSV inclusion (Herpes Simplex Virus)
Cowdry Type A
VZV ( varicella-zoster virus) inclusion
Cowdry Type A
CMV (cytomegalovirus) inclusion
Owl’s eyes
HPV (Human Papillomavirus) inclusion
Koilocytes
Molluscum contagiosum inclusion
Henderson-Patterson bodies
Pox virus inclusion
Guarnieri bodies
Cytologic Smear (2)
a. Pap Smear
b. Tzanck Smear
Smear for HSV and VZV; multinucleated giant cell
Tzanck Smear
smear for detection of HPV carcinoma
Pap Smear
culture obtained from animals
Primary Cell Culture
subcultivated primary culture; normal human cells
Diploid Cell Culture
Primary cell culture (3)
● Primary Monkey Kidney
● Human Embryonic Kidney
● Rhesus Kidney
Diploid Cell Culture (3)
MRC-5
WI-38
Human Diploid Fibroblast (HDF)
immortal cell lines; cancer cells
Continuous Cell Culture
Continuous Cell Culture (4)
HeLa
Hep2
A-549
AGMK
Serology (3)
a. Neutralization
b. Complement fixation
c. Hemagglutination inhibition
detects blockade of cytopathic effects
Neutralization
for influenza and rubella
Hemagglutination inhibition
