Staphylococcus Flashcards

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1
Q

Physical Characteristics of Staphylococcus

A

gram positive cocci in pairs

short chain and clusters

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2
Q

Oxygen requirement and enzyme test positivity of Staphylococcus

A

aerobic and facultatively anaerobic
catalase positive
oxidase negative

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3
Q

Reproduction and Motility of Staphylococcus

A

Non motile
Non spore forming
Fermentative

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4
Q

Pigment present in Staphylococcus

A
Carotenoid pigment
(S. aureum=gold)
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5
Q

Habitat of Staphylococcus

A

Commensal of the skin and mucous membranes
Upper respiratory and digestive tracts of warm blooded animals

Normal in nasal membrane, nasopharynx, skin, perineum, GI tract genital tract

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6
Q

S. aureus

A
Bovine - Mastitis, 
Canine - Pyoderma, UTI
Equine - Botryomycosis
Feline - UTI
Porcine - Mastitis. Metritis
Fowl - Bumblefoot
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7
Q

S. hyicus

A

Porcine - Exudative epidermitis (Greasy Pig Disease)

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8
Q

S. intermedius

A

Canine - Pyoderma

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9
Q

S. delphini

A

Dolphin - Purulent skin lesion

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10
Q

S. schleiferi ssp. coagulans

A

Canine - Otitis Externa

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11
Q

Virulence Factors of Staphylococcus

A
Hemolysin
Leukocidin
Enterotoxin
Toxic Shock Syndrome Toxin (TSST 1)
Exfoliatin (epidermolytic toxin)
Hyaluronidase
Lipase
Staphylokinase
Coagulase
Siderophore
Carotenoids
Catalase
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12
Q

Catalase negative and anaerobic Staphylococcus

A

S. saccharolyticus

S. aureus ssp. anaerobius

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13
Q

Different classes of Hemolysin

A
Alpha
Beta
Delta
Epsilon
Gamma
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14
Q

Hemolysis type of Alpha toxin and action

A

Complete Hemolysis
Beta Hemolysis

Causes spasm of smooth vascular muscle, vasoconstriction, dermonecrotizing, lethal, osmotic lysis

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15
Q

Hemolysis type of Beta toxin and action

A
Partial Hemolysis (Complete if cold)
Alpha Hemolysis

Hot cold hemolysis
Degrades sphingomyelin
In bacteriophange gene

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16
Q

Hemolysis type of Delta toxin

A

Complete

Damage cells in the vessels

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17
Q

Hemolysis type of Epsilon and Gamma toxin

A

Epsilon - Complete

Gamma - Partial

18
Q

Causes pore formation on WBC, kills PMN and macrophanges

A

Leukocidin

19
Q

Types of Enterotoxins

A

A, B, C1-3, D, E, G, H, I

Present in many S. aureus and S. intermedius
Stimulate emetic centers of the brain

20
Q

Enterotoxin associated with Staphylococcus food poisoning in humans

A

Enterotoxin A

21
Q

A non emetic, pseudoenterotoxin, analogous to pyrogenic toxin of strep, superantigen, can cause tolerance

A

Toxic Shock syndrome Toxin (TSST 1)

22
Q

Causes staphylococcal skin syndrome (SSS) and Exudative epidermitis

A

Exfoliatin (epidermolytic toxin)

ShET - pigs
SET - humans

23
Q

Present when slight rubbing of the skin results in exfoliation of the outermost layer. Detachment pf epidermis from the skin

A

Nikolsky’s Sign

24
Q

Found in the cell wall that binds immunoglobulin via the nonspecific Fc receptor, disrupts opsonization and phagocytosis

A

Protein A

25
Q

When present it offers a physical barrier to phagocytic ingestion by masking immunoglobulin Fc site

A

capsule

26
Q

Degrades urea converting it into ammonia, makes urine more alkaline
Damages mucosa of epithelium
Causes urinary stone formation

A

Urease

27
Q

Breaks down hyaluronic acid

A

Hyaluronidase

28
Q

Cleaves either DNA or RNA

A

Nuclease

29
Q

Hydrolyses lipids, fats, and oils

A

Lipases

30
Q

Causes lysis of clots in Staphylococcus infection

A

Staphylokinase (fibrinolysin)

31
Q

Coagulates plasma (staphylothrombin)

A

Coagulase (clumping factor)

32
Q

Responsible for iron acquisition from extracellular source

A

Siderophore (staphyloferrin B)

33
Q

Detoxifies reactive oxygen compounds and gives a golden yellow pigment

A

Carotenoids

34
Q

Destroys H2O2 produced by phagocytes

A

catalase

35
Q

The predominant pattern of Staphylococcus infections

A

Suppuration and Abscess formation

36
Q

Stages of abscess formation

A
  1. Acute inflammation
  2. Chemotactic factors
  3. Outpouring of large amounts of lysosomal enzymes from damages neutrophils
  4. Inflammatory area walled off by thick-walled fibrin capsule
37
Q

Resistance of Staphylococcus

A

Drying for weeks
Heating up to 60°C for 30 minutes
pH fluctuations from 9.5 to 4
7.5% salt concentration

38
Q

Sample collection for Staphylococcus

A

Aspirate from unopened lesions, milk samples, blood, urine

39
Q

Isolation and Identification of Staphylococcus

A

Blood Agar Plates + 15mg Nalidixic Acid/10mg Colistin

Mannitol Salt Agar

Baird Parker Agar

40
Q

Remaining useful antibiotic against Staphylococcus

A

Vancomycin

But vancomycin resistance has been documented