Stains and Cultures

Question 1

Giemsa

Answer 1

Chlamydia
Borrelia
Rickettsiae
Trypanosomes
Plasmodium

Question 2

PAS

Answer 2

stains glycogen, mucopolysaccharides

Whipple’s disease - Tropheryma whipplei

Question 3

Ziehl-Neelsen

Answer 3

Acid-fast bacteia
Nocardia
Mycobacterium

Question 4

India ink

Answer 4

cryptococcus neoformans

Question 5

Silver stain

Answer 5

Fungi
Legionella
H. pylori

Question 6

Chocolate agar

Answer 6

with factors V (NAD+) and X (hematin)

H. influenzae

Question 7

Thayer-Martin agar

Answer 7

VPN:
Vancomycin - inhibit gram-positive organisms

Polymyxin - inhibits gram-negative organism expect Neisseria

Nystatin - inhibit fungi

Neisseria

Question 8

Bordet-Gengou agar

Answer 8

B. pertussis

Question 9

Tellurite plate, Loffler’s media

Answer 9

C. diphtheriae

Question 10

Lowenstein-Jensen agar

Answer 10

M. tuberculosis

Question 11

Eaton’s agar

Answer 11

M. pneumoniae

Question 12

MacConkey’s agar

Answer 12

lactose-fermenting enterics - pink colonies

Question 13

Eosin-methylene blue (EMB) agar

Answer 13

E. coli - green matallic sheen

Question 14

Charcoal yeast extract agar

Answer 14

buffered with cysteine and iron

Legionella

Question 15

Sabouraud’s agar

Answer 15

Fungi