Staining table 3 Flashcards
STAINING TECNIQUE
Ralph’s Method - Lephne-PickworthBenzidine Technique - Gomori’s Application of Perl’s Prussian Blue Reaction - QuirekeTirmam-Schmelzer Method - Turnbull blue Reaction - Katlik’s Ferric Iron Method - Lillie’s Ferrous Iron Method Masson-Fontana Ammoniacal Silver Reaction - Bodian’s Method - Kinyoun’s Acid Fast Stain Ziehl-Neelsen - Levaditi’s Method - Schieifstein’s Method
FIXATION
Ralph’s Method
Alcohol Carnoy’s Fluid
Ziehl-Neelsen
Schieifstein’s Method
Alcohol Carnoy’s Fluid - Formalin or Alcohol or Bouins Fluids - Formalin or Mercuric Fixative - Zenker’s Fluid
SPECIMEN
HEMOGLOBIN
HEMOSIDERIN
PARRIC IRON
FERROUS IRON
BILIRUBIN
MELANIN
NERVE FIBERS AND NERVE ENDINGS
ACID FAST BACILLI
SPIROCHETES
NEGRI BODIES
HEMOGLOBIN REAGENTS
Bensidine HydrogenPeoxide
Nuclear Fast Red
Benzidine-nitroprusside solution
Hydrogen Peroxide
Neutral Red
HEMOSIDERIN REAGENTS
10% Potassium Ferrocyanide 10% HCL
PARRIC IRON REAGENTS
Ammonium sulfate HCL,
Potassium Ferricyanide
FERROUS IRON REAGENTS
Potassium Ferricyanide
BILIRUBIN REAGENTS
Alum Ferrichloride acid Brillian Yellow Ethanol
MELANIN REAGENTS
Ferrous sulfate
Potassium
Ferrycyanide
Acetic Acid
Gram’s iodine Silver nitrate Ammonia water Sodium thiosulfate Gold chloride alcohol
NERVE FIBERS AND NERVE ENDINGS REAGENTS
Protargol Solution,
Gold Chloride
Oxalic Acid
Aniline Blue
ACID FAST BACILLI REAGENTS
Carbolfuchsin, Acid Alcohol, Methylene blue Carbolfuchsin, Acid Alcohol, Sulfuric acid, Methylene blue
SPIROCHETES REAGENTS
Siver Nitrate Pyrogallic acid
NEGRI BODIES REAGENTS
Schieifsteinst. Iodine sodium thiosulfate
RESULT
Hemoglobin- dark brown Hemoglobin-deep blue - Ferric Iron-blue - Parric iron-blue - Ferrous iron-blue - Bilirubin-green - Melanin-deep green Melanin-black - Nerve fibers-black - AFB-bright red AFB-bright red
