Fixatives

Question 1

Use exceptionally bloody specimen such as infarcts and congested spleen.

Use for unusual tumors such as _ and _

Use for virals inclusion such as __

Recommended for the preservation of tissue on which __ is done

Never use __ OR __ when handling any tissue fixed in mercurial fixatives

Answer 1

ZENKER’S FLUID

rhabdomyosarcoma and malignant teratomas

Negri bodies

Feulgen plasma reaction

metal forceps or metal containers

Question 2

ZENKER’S FLUID

Excellent ___ after this fixatives

Omit the ___ and add it to the stock solution when read to use

An ___ is formed with this mercuric fixative.

Remove during staining sequence with the ___

For excellent ____, tissue must be fixed in Zenker’s Fluid

Answer 2

trichomes

acetic acid

artefact pigment

iodine-sodium thiosulfate sequence

phosphotungstic acid hematoxylin stains

Question 3

This fixative is known as Zenker’s formol since it uses the same basic stock solution and formalin is not added to the stock until just before use to prevent a ___ precipitate from forming

An artifact pigment is formed with this mercuric fixative, which must be removed during staining sequence, with the __sequence

Excellent for __ organs

Excellent for __

Answer 3

HELLY’S FLUID

dark brown

iodine thiosulfate

blood forming

intercalated discs

Question 4

Excellent for bone marrow including the preservation of RBC

Answer 4

HELLY’S FLUID

Question 5

RNA must be fixed in this fixatives if the __ technique is done

_ _ _ is preserved with fixation

Answer 5

CARNOY’S FLUID

methyl green pyronine

Glycogen storage disease

Question 6

Never use this fixative if acid fast bacilli are to be demonstrated bacilli are rendered non-acid fast ____ hemolyzes RBC and dissolves acid soluble cell granules and pigments

Answer 6

Carnoy’s fluid

Question 7

___ granules are well preserved in Carnoy’s fluid

Answer 7

Nissl granules

Question 8

The most widely used fixing agent for pathologic histology

Answer 8

FORMALIN

Question 9

__ is commercially available at 37%to 40% gas in water, sold as formalin. These fluids are regarded as 100% formalin and the

10% solution contains __ of the ___ gas and

20% solution contains __of the___gas.

Answer 9

Formaldehyde

4. 1% to 4.5%
5. 2% to 9%

Question 10

Solutions of formalin diluted with the distilled water are commonly acid from small amounts of ___ as a manufacturing impurity or from oxidation of gas

Answer 10

formic acids

Question 11

__ should not be prepared by storage over calcium carbonate or magnesium carbonate since the fluid drawn off for fixation promptly becomes acid

Answer 11

NBF

Question 12

___ is the best fixative for tissue for fat stains since fewer artifacts are seen in ___ stains after this fixatives

Answer 12

10% formalin

oil red O

Question 13

Fixation in 10% Formalin is must for the ___ technic for __

Answer 13

Warthin Starry

spirochete

Question 14

___ or ___ is an excellent fixative for preserving glycogen

Answer 14

Alcohol formalin or alcoholic picro formalin

Question 15

For pH, best result are obtained if the pH of the formalin is _. Formalin pigment in solutions at pH levels below, pH levels above pH 8.0. Both pigments are dissolved either by ___ or by _____ in __alcohol

Answer 15

7.0

alcoholic picric acid

1% potassium hydroxide - 80%

Question 16

An excellent balanced fixative for routine surgical material

Answer 16

BOUIN’S FLUID

Question 17

___ should be used in grossly fatty tissue, especially fat containing __, __ and __.. Since this is a fat solvent, do not use for tissue on which fat stains may be required

Answer 17

Bouin’s fat fixative

lymph nodes, fatty breast tissue and lipomas

Question 18

BOUIN’S FLUID

Remember to remove _ color with either ____ in 70% alcohol during the staining sequence

Excellent connective tissue stains after this fixative, particularly trichomes

Answer 18

yellow

70% alcohol or saturated lithium carbonate

Question 19

SPECIAL FIXATIVES

Answer 19

ADRENAL
OSMIC ACID FIXATIVES- 
URIC ACID- 
CNS-
PHOSPHOLIPIDS
TISSUE ENZYMES( phosphatases and lipases)

Question 20

ADRENAL- fix in ___ which is a potassium dichromate fixative

Answer 20

Orth’s fluid

Question 21

PHOSPHOLIPIDS- fix in ____

Answer 21

Baker’s formol-calcium

Question 22

TISSUE ENZYMES( phosphatases and lipases) – fix in __

Answer 22

Acetone

Question 23

CNS- fix in___ depending on the staining technique

Answer 23

formalin ammonium bromide or NBF

Question 24

URIC ACID- fix in __, destroyed by _ fixatives

Answer 24

Absolute alcohol

aqueous

Question 25

OSMIC ACID FIXATIVES- will ___ ___ and demonstrate them in this manner

Answer 25

blacken triglycerides