MUCOPOLYSACCHARIDE

Question 1

SUBSTANCE/SPECIMEN - FIXATION

Colloidal Iron Technique

Alcian Blue Stain w/ or w/o Hyaluronidase

Metchromatic Staining with Toluidine Blue

Answer 1

Mucopolysaccharide - 10% Formalin

Acid Mucopolysaccharide - 10% Formalin

Acid Mucopolusaccharide - Alcohol or Formalin

Question 2

REAGENTS - RESULTS

Colloidal Iron Technique

Alcian Blue Stain w/ or w/o Hyaluronidase

Metchromatic Staining with Toluidine Blue

Answer 2

Acetic Acid potassium
Ferrocyanide colloidal iron solution

Acetic acid alcian blue solution,
Kernechtrot solution Acid

mucopolysaccharide- blue

-
0.5% aqueous Toluidine Blue

Acid mucopolysaccharide-red to pink