FATS AND FATTY ACIDS Flashcards
1
Q
STAINING TECHNIQUE
A
Clark Scharlach R Stain Osmium Tetroxide Stain Oil Red O Stain Sudan Black B Nile Blue Lillie’s Sulfuric Acid Nile Blue Stain
2
Q
SUBSTANCE/SPECIMEN - FIXATION
Clark Scharlach R Stain Osmium Tetroxide Stain Oil Red O Stain Sudan Black B Nile Blue Lillie’s Sulfuric Acid Nile Blue Stain
A
Grossly Fat Tissues - 10% Formalin
Fat - 10% Formalin
Fat( Neutral Fats) - 10% Formalin
Fat - 10% Formalin
Fatty Acids - 10% Formalin
Fatty Acids - Frozen
3
Q
Clark Scharlach R Stain
A
Scharlach R Solution
Gelatin Jelly
Fat-orange to red
4
Q
Osmium Tetroxide Stain
A
Osmic Acid ,
Glycerin Jelly
Fat-black
5
Q
Oil Red O Stain
A
Oil red O,
Glycerin Jelly,
Harris Hematoxylin
Fat-orange to bright red
6
Q
Sudan Black B
A
Sudan Black B,
Propylene Glycol,
Nuclear Fast
Fat- black or blue black
7
Q
Nile Blue
A
Nile Blue,
Nile Red
Neutral fats-pink or red
Acidic lipids-blue
8
Q
Lillie’s Sulfuric Acid Nile Blue Stain
A
Nile Blue,
Sulfuric Acid,
Nile Red
Fatty acid- dark blue,
Neutral fat - pink to red
