Stages Of PCR

Question 1

Stage 1:

Answer 1

DNA is heated to between 92 and 98 degrees to separate the DNA strands

Question 2

Stage 2:

Answer 2

DNA is cooled to between 50 and 65 degrees to allow primers to bind to target sequences.

Question 3

Stage 3:

Answer 3

Heated to between 70 and 80 degrees for heat tolerant DNA polymerase to replicate the region of DNA.