Sputum

Question 1

Testing for:

Answer 1

Streptococci pnemonicocci, staphylococci, enterobacteria non fermenter, haemophilus, yeast, mycobacteria

Question 2

Streptococci pneumonia

Answer 2

Culture: Columbia CNA
Detection: Hemolysis
                  /            \
            (Alpha)         (Beta)
           Pnemo kit     Strepto kit
               /                   \
     S. Pneumonia    Strep A,B, C, D,F,G
Antibiotic susceptibly: ATB Strep

Question 3

Staphylococci

Answer 3

Culture: mannitol salt agar
Detection: Staph kit
                      |
                 S. Aureus
Antibiotic susceptibly: ATB rapid staph

Question 4

Enterobacteria non fermenters

Answer 4

Culture: mckonkey
Detection: Api
                      |
                 Species (67/110)
Antibiotic susceptibly: ATB G

Question 5

Haemophilus influenza

Answer 5

Culture: chocolate + polyvitex +bacterium co2 atm
Detection: Api
                      |
                 Haemophilus 4 species  
Antibiotic susceptibly: ATB Haemophilus

Question 6

Yeast

Answer 6

Culture: Sabrougard
Detection: Albicans ID
/ \
(+Ve) (-Ve)

           /                   \
 C.albicans             Api Antibiotic susceptibly: ATB Fungus

Question 7

Mycobacterium

Answer 7

Culture: sputum diagnostics + decontamination
Lowenstein Jensen and colectos
Detection: Mycobacteria

Question 8

Transport and collection

Answer 8

• collect sample ASAP
• morning
• prior ( gargle water) ; reduce oral bacteria–> so no mouthwash
• cough deeply ( sputum not saliva)
• acid fast cultures (3 times specimen :8-24 hours)
• sterile simple container
• transport at room temp within 24 hour collection