Spectrophotometry and Wavelength Selection Flashcards
Serum protein concentration
7.0 g/100 mL
Isolate a narrower range of wavelength
Monochromator systems
Graphs to help in selecting the proper wavelength
Spectral-absorbance
Spectral-transmittance
Wavelength
425 nm
It measures light intensity without consideration if wavelength.
Photometer
Essential in obtaining reliable laboratory results that are necessary in the diagnosis and management of diseases
Fundamental understanding of the principles of spectrophotometry
Wavelength selection
Maximum absorption
Color complimentary to the color of the solution being measured
Reaction of serum protein
Biuret reaction
Equipment used to measure absorbed light or transmitted light
Photometers
Spectrophotometers
Examples of monochromators
Diffraction grating
Prism
It measures light intensity in a narrower wavelength.
Spectrophotometer
Absorbed light
Optical density
Basis of analytical methodologies
Measurements of radiant energy that is absorbed or transmitted