Spectrophotometry and Wavelength Selection Flashcards

0
Q

Serum protein concentration

A

7.0 g/100 mL

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1
Q

Isolate a narrower range of wavelength

A

Monochromator systems

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4
Q

Graphs to help in selecting the proper wavelength

A

Spectral-absorbance

Spectral-transmittance

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5
Q

Wavelength

A

425 nm

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6
Q

It measures light intensity without consideration if wavelength.

A

Photometer

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9
Q

Essential in obtaining reliable laboratory results that are necessary in the diagnosis and management of diseases

A

Fundamental understanding of the principles of spectrophotometry

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10
Q

Wavelength selection

A

Maximum absorption

Color complimentary to the color of the solution being measured

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12
Q

Reaction of serum protein

A

Biuret reaction

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13
Q

Equipment used to measure absorbed light or transmitted light

A

Photometers

Spectrophotometers

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15
Q

Examples of monochromators

A

Diffraction grating

Prism

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16
Q

It measures light intensity in a narrower wavelength.

A

Spectrophotometer

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22
Q

Absorbed light

A

Optical density

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24
Q

Basis of analytical methodologies

A

Measurements of radiant energy that is absorbed or transmitted

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