Serum Glucose

Question 0

The glucose is stable for 24 hours at 2-8 C if

Answer 0

serum or plasma is prepared within 30 minutes after collection

Question 1

Test is not influenced by

Answer 1

Uric acid
Ascorbic acid
Glutathione
Anticoagulants
Bilirubin
Creatinine

Question 2

Reagent stability

Answer 2

1 Unopened - 2-8 C

2 Opened - 2-8 C (6 weeks) or 15-25 (2 weeks)

Question 2

Specimen

Answer 2

Serum

Question 4

Wavelength

Answer 4

500 nm, Hg 546 nm

Question 5

Incubation period

Answer 5

10 minutes (20-25 C)
5 minutes (37 C)

Question 6

Calculation of the glucose concentration

Answer 6

(Review)

Question 7

Linearity

Answer 7

Glucose concentration = 400 mg/dL or 22.2 mmol/L

Question 8

Reaction principle

Answer 8

1 The glucose is determined after enzymatic oxidation in the presence of glucose oxidase

Glucose + Oxygen + Water -> Gluconic Acid + Hydrogen Peroxide

2 The formed hydrogen peroxide reacts under catalysis of peroxidase with phenol and 4-aminophenazone to a red-violet quinoneimine dye as indicator

2Hydrogen Peroxide + 4-aminophenazone + phenol -> quinoneimine + 4Water

Question 9

Reagents

Answer 9

Phosphate buffer (pH 7.5)
4-aminophenazone
Phenol
Glucose oxidase
Peroxidase
Mutarotase
Stabilizers

Question 10

Reference range

Answer 10

Serum, plasma (fasting) = 70-100 mg/dL or 3.9-5.6 mmol/L

Question 11

Temperature

Answer 11

20-25 C or 37 C

Question 12

Optical path

Answer 12

1 cm