Serum Cholesterol

Question 0

Range

Answer 0

Desirable: /= 240 mg/dL

Question 1

Incubation period

Answer 1

20 minutes at 20-25 C

10 minutes at 37 C

Question 2

Wavelength

Answer 2

500 nm, Hg 546 nm

Question 3

Temperature

Answer 3

20-25 C or 37 C

Question 4

Calculation of the cholesterol concentration

Answer 4

(Review)

Question 5

Reaction principle

Answer 5

1 Serum cholesterol is determined after enzymatic hydrolysis and oxidation
Cholesterol ester + Water -> Cholesterol + Fatty Acid

2
Cholesterol + Oxygen -> cholestene-3-one + Water

3 The indicator quinoneimine is formed from hydrogen peroxide and 4-aminoantipyrine in the presence of phenol and peroxidase
2Hydrogen peroxide + 4-aminoantipyrine + phenol -> quinoneimine + 4Water

Question 5

Specimen

Answer 5

Serum

EDTA- or heparinized plasma

Question 7

Recommend a complete lipoprotein profile (TC, LDL-C, HDL-C, and TAG)

Answer 7

National Cholesterol Education Program (NCEP) Expert Panel on Detection, Evaluation, and Treatment of High Blood Cholesterol in Adults (Adult Treatment Panel III or ATP III)

Question 8

Repeat testing at least once every ____

Answer 8

5 years

Question 9

Reagent stability

Answer 9

1 Unopened - 2-8 C (expiry date)

2 Opened - 2-8 C (6 weeks) or 15-25 C (2 weeks)

Question 10

Optical path

Answer 10

1 cm

Question 11

Linearity

Answer 11

The test is linear up to a cholesterol concentration of 750 mg/dL or 19.3 mmol/L.

Question 12

Reagents

Answer 12

Phosphate buffer (6.5)
4-aminoantipyrine
Phenol
Peroxidase
Cholesterolesterase
Cholesteroloxidase
Sodium azide