SPECIMEN FOR DIFFERENT LABORATORY TEST Flashcards
CBC
Edta whole blood
Platelet count and Reticulocyte count
EDTA
Modified Westergren method of ESR
Edta whole blood
Sickling test (Sodium metabisulfite)
Edta whole blood
Heinz body preparation
Edta whole blood
Blood smear preparation for parasites
Edta whole blood
Red cell zinc protoporphyrin
Edta whole blood
Hemoglobin electrophoresis at pH 8.6 (hemolysate)
Edta whole blood
Hemoglobin electrophoresis at acid pH (hemolysate)
Edta whole blood
Hemoglobin A2 quantitation (hemolysate)
Edta whole blood
Hemoglobin F quantitation (hemolysate)
Edta whole blood
Hemoglobin H preparation
Edta whole blood
Heat precipitation test (unstable hemoglobin)
Edta whole blood
Ascorbate cyanide screening test (for pentose phosphate pathway deficiencies)
Edta whole blood
G6PD fluorescent screening test
Edta whole blood
Pyruvate kinase test
Edta whole blood
Glutathione reductase test
Edta wholw blood
Methemoglobin quantitation
Edta whole blood
EOFT
HEPARINIZED WHOLE BLOOD
Solubility test
HEPARINIZED WHOLE BLOOD
Sickling test (sodium metabisulfite)
HEPARINIZED WHOLE BLOOD
• Nitroblue tetrazolium dye test
HEPARINIZED WHOLE BLOOD
Heinz body preparation
HEPARINIZED WHOLE BLOOD
Red cell zinc protoporphyrin
HEPARINIZED WHOLE BLOOD
Hemoglobin electrophoresis at pH 8.6 (hemolysate)
HEPARINIZED WHOLE BLOOD
Heat precipitation test (unstable hemoglobins)
HEPARINIZED WHOLE BLOOD
Ascorbate cyanide test (for pentose phosphate pathway def.)
HEPARINIZED WHOLE BLOOD
G6PD fluorescent screening test
HEPARINIZED WHOLE BLOOD
Pyruvate kinase test
HEPARINIZED WHOLE BLOOD
Glutathione reductase test
HEPARINIZED WHOLE BLOOD
Methemoglobin quantitation
HEPARINIZED WHOLE BLOOD
LE Prep
HEPARINIZED WHOLE BLOOD
Prussian blue stain
AIR DRIED BLOOD OR BONE MARROW SMEARS
Periodic Acid Schiff
AIR DRIED BLOOD OR BONE MARROW SMEARS
Sudan Black B
AIR DRIED BLOOD OR BONE MARROW SMEAR
Acid phosphatase stain (with tartrate resistance)
AIR DRIED BLOOD OR BONE MARROW SMEAR
Non specific esterase stain
AIR DRIED BLOOD OR BONE MARROW SMEARS
Specific esterase stain
AIR DRIED BLOOD OR BONE MARROW SMEARS
Original Westergren method of ESR
CITRATED WHOLE BLOOD
Solubility test
CITRATED WHOLE BLOOD
Red cell zinc protoporphyrin
CITRATED WHOLE BLOOD
Red cell zinc protoporphyrin
CITRATED WHOLE BLOOD
Sugar Water Screening test for PNH
CITRATED WHOLE BLOOD
Sucrose Hemolysis test for PNH
CITRATED WHOLE BLOOD
Prothrombin time
• Activated Partial Thromboplastin time
PLATELET-POOR CITRATED PLASMA
• Stypven time
• Reptilase time
PLATELET-POOR CITRATED PLASMA
Thrombin time
PLATELET-POOR CITRATED PLASMA
Plasma recalcification time
PLATELET-POOR CITRATED PLASMA
Prekallikrein screening test Duckert’s test for Factor XIII
PLATELET-POOR CITRATED PLASMA
Fibrinogen quantitation
PLATELET-POOR CITRATED PLASMA
D-dimer
PLATELET-POOR CITRATED PLASMA
Ethanol gelation test
PLATELET-POOR CITRATED PLASMA
Protamine Sulfate test
PLATELET-POOR CITRATED PLASMA
Euglobulin Clot lysis time
PLATELET-POOR CITRATED PLASMA
Tests for circulating anticoagulants
PLATELET-POOR CITRATED PLASMA
Platelet neutralization procedure
PLATELET-POOR CITRATED PLASMA
Factor VIII inhibitor assay
PLATELET-POOR CITRATED PLASMA
Antithrombin III
PLATELET-POOR CITRATED PLASMA
Plasminogen assay
PLATELET-POOR CITRATED PLASMA
Protein C and protein S tests
PLATELET-POOR CITRATED PLASMA
Haptoglobin
SERUM
Viscosity test
SERUM
EOFT
DEFIBRINATED BLOOD
Autohemolysis test for hereditary spherocytosis
DEFIBRINATED BLOOD
DEFIBRINATED BLOOD
Acid Serum Test for PNH
LAP stain
CAPILLARY BLOOD
Peroxidase stain
CAPILLARY BLOOD
RBC DILUTING FLUIDS
• Hayem’s fluid
• Gower’s fluid
• Toisson’s fluid
• Bethel’s fluid
• Formol-Citrate (Dacie’s fluid)
• 3.8% Na Citrate
Common characteristics of RBC diluting fluid regardless of the name
Isotonic
WBC DILUTING FLUIDS
• 2-3% glacial acetic acid with Gential violet
• 1% hydrochloric acid
• Turk’s solution
PLATELET DILUTING FLUIDS
• Tocantin’s method:
→ Rees-Ecker Diluent uses citrate formaldehyde buffer with brilliant cresyl
blue for light microscopy
• Guy and Leake’s method
→ Uses crystal violet, sodium citrate, and formalin
→ Also used for light microscopy
• 1% ammonium oxalate diluting fluid for phase-contrast microscopy
NEUBAUER COUNTING CHAMBER
• Total Area:
• Depth:
• Total volume:
• Total Area: 9 mm2
• Depth: 0.1 mm or 1/10 mm
• Total volume: 0.9 mm3 or 0.9 μL
SPEIRS-LEVY COUNTING CHAMBER
• Total Area:
• Depth:
• Total Volume:
• Total Area: 10mm3
• Depth: 0.2mm
• Total Volume: 2.0mm3
FUCHS-ROSENTHAL COUNTING CHAMBER
• Total Area:
• Depth:
• Total Volume:
• Total Area: 16mm2
• Depth: 0.2mm
• Total Volume: 3.2mm3
3° TERTIARY SQUARE (SMALL SQ. ON THE CORNER) area
𝟎. 𝟎𝟔𝟐𝟓𝒎𝒎𝟐
3° TERTIARY SQUARE (SMALL SQ. ON THE CENTER) area
𝟎. 𝟎𝟒𝒎𝒎𝟐
4° QUATERNARY SQUARE (SMALLEST SQ.)
𝟎. 𝟎𝟎𝟐𝟓𝒎𝒎𝟐
Rbc counting area
• Usually used is the 5 small squares on the central square
• There are 5 small squares and each square has an area of 0.04 mm2
WBC counting area
• We count on 4 large corner squares and each square has an area of 1 mm2
→ What is the general area for WBC counting?
4 𝑥 1 𝑚𝑚2 = 𝟒 𝒎𝒎𝟐
Platelet counting area
• We only use the entire large central square, which has an area of 1 mm2
• There are 25 small squares and each square has an area of 0.04 mm2
→ What is the general area for platelet counting?
25 𝑥 0.04 𝑚𝑚2 = 𝟏 𝒎𝒎𝟐