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Hema MTAP > SPECIMEN FOR DIFFERENT LABORATORY TEST > Flashcards

SPECIMEN FOR DIFFERENT LABORATORY TEST Flashcards

1
Q

CBC

A

Edta whole blood

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2
Q

Platelet count and Reticulocyte count

A

EDTA

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3
Q

Modified Westergren method of ESR

A

Edta whole blood

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4
Q

Sickling test (Sodium metabisulfite)

A

Edta whole blood

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5
Q

Heinz body preparation

A

Edta whole blood

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6
Q

Blood smear preparation for parasites

A

Edta whole blood

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7
Q

Red cell zinc protoporphyrin

A

Edta whole blood

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8
Q

Hemoglobin electrophoresis at pH 8.6 (hemolysate)

A

Edta whole blood

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9
Q

Hemoglobin electrophoresis at acid pH (hemolysate)

A

Edta whole blood

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10
Q

Hemoglobin A2 quantitation (hemolysate)

A

Edta whole blood

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11
Q

Hemoglobin F quantitation (hemolysate)

A

Edta whole blood

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12
Q

Hemoglobin H preparation

A

Edta whole blood

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13
Q

Heat precipitation test (unstable hemoglobin)

A

Edta whole blood

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14
Q

Ascorbate cyanide screening test (for pentose phosphate pathway deficiencies)

A

Edta whole blood

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15
Q

G6PD fluorescent screening test

A

Edta whole blood

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16
Q

Pyruvate kinase test

A

Edta whole blood

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17
Q

Glutathione reductase test

A

Edta wholw blood

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18
Q

Methemoglobin quantitation

A

Edta whole blood

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19
Q

EOFT

A

HEPARINIZED WHOLE BLOOD

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20
Q

Solubility test

A

HEPARINIZED WHOLE BLOOD

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21
Q

Sickling test (sodium metabisulfite)

A

HEPARINIZED WHOLE BLOOD

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22
Q

• Nitroblue tetrazolium dye test

A

HEPARINIZED WHOLE BLOOD

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23
Q

Heinz body preparation

A

HEPARINIZED WHOLE BLOOD

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24
Q

Red cell zinc protoporphyrin

A

HEPARINIZED WHOLE BLOOD

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25
Hemoglobin electrophoresis at pH 8.6 (hemolysate)
HEPARINIZED WHOLE BLOOD
26
Heat precipitation test (unstable hemoglobins)
HEPARINIZED WHOLE BLOOD
27
Ascorbate cyanide test (for pentose phosphate pathway def.)
HEPARINIZED WHOLE BLOOD
28
G6PD fluorescent screening test
HEPARINIZED WHOLE BLOOD
29
Pyruvate kinase test
HEPARINIZED WHOLE BLOOD
30
Glutathione reductase test
HEPARINIZED WHOLE BLOOD
31
Methemoglobin quantitation
HEPARINIZED WHOLE BLOOD
32
LE Prep
HEPARINIZED WHOLE BLOOD
33
Prussian blue stain
AIR DRIED BLOOD OR BONE MARROW SMEARS
34
Periodic Acid Schiff
AIR DRIED BLOOD OR BONE MARROW SMEARS
35
Sudan Black B
AIR DRIED BLOOD OR BONE MARROW SMEAR
36
Acid phosphatase stain (with tartrate resistance)
AIR DRIED BLOOD OR BONE MARROW SMEAR
37
Non specific esterase stain
AIR DRIED BLOOD OR BONE MARROW SMEARS
38
Specific esterase stain
AIR DRIED BLOOD OR BONE MARROW SMEARS
39
Original Westergren method of ESR
CITRATED WHOLE BLOOD
40
Solubility test
CITRATED WHOLE BLOOD
41
Red cell zinc protoporphyrin
CITRATED WHOLE BLOOD
42
Red cell zinc protoporphyrin
CITRATED WHOLE BLOOD
43
Sugar Water Screening test for PNH
CITRATED WHOLE BLOOD
44
Sucrose Hemolysis test for PNH
CITRATED WHOLE BLOOD
45
Prothrombin time • Activated Partial Thromboplastin time
PLATELET-POOR CITRATED PLASMA
46
• Stypven time • Reptilase time
PLATELET-POOR CITRATED PLASMA
47
Thrombin time
PLATELET-POOR CITRATED PLASMA
48
Plasma recalcification time
PLATELET-POOR CITRATED PLASMA
49
Prekallikrein screening test Duckert's test for Factor XIII
PLATELET-POOR CITRATED PLASMA
50
Fibrinogen quantitation
PLATELET-POOR CITRATED PLASMA
51
D-dimer
PLATELET-POOR CITRATED PLASMA
52
Ethanol gelation test
PLATELET-POOR CITRATED PLASMA
53
Protamine Sulfate test
PLATELET-POOR CITRATED PLASMA
54
Euglobulin Clot lysis time
PLATELET-POOR CITRATED PLASMA
55
Tests for circulating anticoagulants
PLATELET-POOR CITRATED PLASMA
56
Platelet neutralization procedure
PLATELET-POOR CITRATED PLASMA
57
Factor VIII inhibitor assay
PLATELET-POOR CITRATED PLASMA
58
Antithrombin III
PLATELET-POOR CITRATED PLASMA
59
Plasminogen assay
PLATELET-POOR CITRATED PLASMA
60
Protein C and protein S tests
PLATELET-POOR CITRATED PLASMA
61
Haptoglobin
SERUM
62
Viscosity test
SERUM
63
EOFT
DEFIBRINATED BLOOD
64
Autohemolysis test for hereditary spherocytosis
DEFIBRINATED BLOOD
65
DEFIBRINATED BLOOD
Acid Serum Test for PNH
66
LAP stain
CAPILLARY BLOOD
67
Peroxidase stain
CAPILLARY BLOOD
68
RBC DILUTING FLUIDS
• Hayem’s fluid • Gower’s fluid • Toisson’s fluid • Bethel’s fluid • Formol-Citrate (Dacie’s fluid) • 3.8% Na Citrate
69
Common characteristics of RBC diluting fluid regardless of the name
Isotonic
70
WBC DILUTING FLUIDS
• 2-3% glacial acetic acid with Gential violet • 1% hydrochloric acid • Turk’s solution
71
PLATELET DILUTING FLUIDS
• Tocantin’s method: → Rees-Ecker Diluent uses citrate formaldehyde buffer with brilliant cresyl blue for light microscopy • Guy and Leake’s method → Uses crystal violet, sodium citrate, and formalin → Also used for light microscopy • 1% ammonium oxalate diluting fluid for phase-contrast microscopy
72
NEUBAUER COUNTING CHAMBER • Total Area: • Depth: • Total volume:
• Total Area: 9 mm2 • Depth: 0.1 mm or 1/10 mm • Total volume: 0.9 mm3 or 0.9 μL
73
SPEIRS-LEVY COUNTING CHAMBER • Total Area: • Depth: • Total Volume:
• Total Area: 10mm3 • Depth: 0.2mm • Total Volume: 2.0mm3
74
FUCHS-ROSENTHAL COUNTING CHAMBER • Total Area: • Depth: • Total Volume:
• Total Area: 16mm2 • Depth: 0.2mm • Total Volume: 3.2mm3
75
3° TERTIARY SQUARE (SMALL SQ. ON THE CORNER) area
𝟎. 𝟎𝟔𝟐𝟓𝒎𝒎𝟐
76
3° TERTIARY SQUARE (SMALL SQ. ON THE CENTER) area
𝟎. 𝟎𝟒𝒎𝒎𝟐
77
4° QUATERNARY SQUARE (SMALLEST SQ.)
𝟎. 𝟎𝟎𝟐𝟓𝒎𝒎𝟐
78
Rbc counting area
• Usually used is the 5 small squares on the central square • There are 5 small squares and each square has an area of 0.04 mm2
79
WBC counting area
• We count on 4 large corner squares and each square has an area of 1 mm2 → What is the general area for WBC counting? 4 𝑥 1 𝑚𝑚2 = 𝟒 𝒎𝒎𝟐
80
Platelet counting area
• We only use the entire large central square, which has an area of 1 mm2 • There are 25 small squares and each square has an area of 0.04 mm2 → What is the general area for platelet counting? 25 𝑥 0.04 𝑚𝑚2 = 𝟏 𝒎𝒎𝟐

Hema MTAP (6 decks)

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