SPECIMEN FOR DIFFERENT LABORATORY TEST Flashcards

1
Q

CBC

A

Edta whole blood

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2
Q

Platelet count and Reticulocyte count

A

EDTA

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3
Q

Modified Westergren method of ESR

A

Edta whole blood

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4
Q

Sickling test (Sodium metabisulfite)

A

Edta whole blood

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5
Q

Heinz body preparation

A

Edta whole blood

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6
Q

Blood smear preparation for parasites

A

Edta whole blood

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7
Q

Red cell zinc protoporphyrin

A

Edta whole blood

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8
Q

Hemoglobin electrophoresis at pH 8.6 (hemolysate)

A

Edta whole blood

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9
Q

Hemoglobin electrophoresis at acid pH (hemolysate)

A

Edta whole blood

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10
Q

Hemoglobin A2 quantitation (hemolysate)

A

Edta whole blood

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11
Q

Hemoglobin F quantitation (hemolysate)

A

Edta whole blood

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12
Q

Hemoglobin H preparation

A

Edta whole blood

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13
Q

Heat precipitation test (unstable hemoglobin)

A

Edta whole blood

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14
Q

Ascorbate cyanide screening test (for pentose phosphate pathway deficiencies)

A

Edta whole blood

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15
Q

G6PD fluorescent screening test

A

Edta whole blood

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16
Q

Pyruvate kinase test

A

Edta whole blood

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17
Q

Glutathione reductase test

A

Edta wholw blood

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18
Q

Methemoglobin quantitation

A

Edta whole blood

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19
Q

EOFT

A

HEPARINIZED WHOLE BLOOD

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20
Q

Solubility test

A

HEPARINIZED WHOLE BLOOD

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21
Q

Sickling test (sodium metabisulfite)

A

HEPARINIZED WHOLE BLOOD

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22
Q

• Nitroblue tetrazolium dye test

A

HEPARINIZED WHOLE BLOOD

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23
Q

Heinz body preparation

A

HEPARINIZED WHOLE BLOOD

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24
Q

Red cell zinc protoporphyrin

A

HEPARINIZED WHOLE BLOOD

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25
Q

Hemoglobin electrophoresis at pH 8.6 (hemolysate)

A

HEPARINIZED WHOLE BLOOD

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26
Q

Heat precipitation test (unstable hemoglobins)

A

HEPARINIZED WHOLE BLOOD

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27
Q

Ascorbate cyanide test (for pentose phosphate pathway def.)

A

HEPARINIZED WHOLE BLOOD

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28
Q

G6PD fluorescent screening test

A

HEPARINIZED WHOLE BLOOD

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29
Q

Pyruvate kinase test

A

HEPARINIZED WHOLE BLOOD

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30
Q

Glutathione reductase test

A

HEPARINIZED WHOLE BLOOD

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31
Q

Methemoglobin quantitation

A

HEPARINIZED WHOLE BLOOD

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32
Q

LE Prep

A

HEPARINIZED WHOLE BLOOD

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33
Q

Prussian blue stain

A

AIR DRIED BLOOD OR BONE MARROW SMEARS

34
Q

Periodic Acid Schiff

A

AIR DRIED BLOOD OR BONE MARROW SMEARS

35
Q

Sudan Black B

A

AIR DRIED BLOOD OR BONE MARROW SMEAR

36
Q

Acid phosphatase stain (with tartrate resistance)

A

AIR DRIED BLOOD OR BONE MARROW SMEAR

37
Q

Non specific esterase stain

A

AIR DRIED BLOOD OR BONE MARROW SMEARS

38
Q

Specific esterase stain

A

AIR DRIED BLOOD OR BONE MARROW SMEARS

39
Q

Original Westergren method of ESR

A

CITRATED WHOLE BLOOD

40
Q

Solubility test

A

CITRATED WHOLE BLOOD

41
Q

Red cell zinc protoporphyrin

A

CITRATED WHOLE BLOOD

42
Q

Red cell zinc protoporphyrin

A

CITRATED WHOLE BLOOD

43
Q

Sugar Water Screening test for PNH

A

CITRATED WHOLE BLOOD

44
Q

Sucrose Hemolysis test for PNH

A

CITRATED WHOLE BLOOD

45
Q

Prothrombin time
• Activated Partial Thromboplastin time

A

PLATELET-POOR CITRATED PLASMA

46
Q

• Stypven time
• Reptilase time

A

PLATELET-POOR CITRATED PLASMA

47
Q

Thrombin time

A

PLATELET-POOR CITRATED PLASMA

48
Q

Plasma recalcification time

A

PLATELET-POOR CITRATED PLASMA

49
Q

Prekallikrein screening test Duckert’s test for Factor XIII

A

PLATELET-POOR CITRATED PLASMA

50
Q

Fibrinogen quantitation

A

PLATELET-POOR CITRATED PLASMA

51
Q

D-dimer

A

PLATELET-POOR CITRATED PLASMA

52
Q

Ethanol gelation test

A

PLATELET-POOR CITRATED PLASMA

53
Q

Protamine Sulfate test

A

PLATELET-POOR CITRATED PLASMA

54
Q

Euglobulin Clot lysis time

A

PLATELET-POOR CITRATED PLASMA

55
Q

Tests for circulating anticoagulants

A

PLATELET-POOR CITRATED PLASMA

56
Q

Platelet neutralization procedure

A

PLATELET-POOR CITRATED PLASMA

57
Q

Factor VIII inhibitor assay

A

PLATELET-POOR CITRATED PLASMA

58
Q

Antithrombin III

A

PLATELET-POOR CITRATED PLASMA

59
Q

Plasminogen assay

A

PLATELET-POOR CITRATED PLASMA

60
Q

Protein C and protein S tests

A

PLATELET-POOR CITRATED PLASMA

61
Q

Haptoglobin

62
Q

Viscosity test

63
Q

EOFT

A

DEFIBRINATED BLOOD

64
Q

Autohemolysis test for hereditary spherocytosis

A

DEFIBRINATED BLOOD

65
Q

DEFIBRINATED BLOOD

A

Acid Serum Test for PNH

66
Q

LAP stain

A

CAPILLARY BLOOD

67
Q

Peroxidase stain

A

CAPILLARY BLOOD

68
Q

RBC DILUTING FLUIDS

A

• Hayem’s fluid
• Gower’s fluid
• Toisson’s fluid
• Bethel’s fluid
• Formol-Citrate (Dacie’s fluid)
• 3.8% Na Citrate

69
Q

Common characteristics of RBC diluting fluid regardless of the name

70
Q

WBC DILUTING FLUIDS

A

• 2-3% glacial acetic acid with Gential violet
• 1% hydrochloric acid
• Turk’s solution

71
Q

PLATELET DILUTING FLUIDS

A

• Tocantin’s method:
→ Rees-Ecker Diluent uses citrate formaldehyde buffer with brilliant cresyl
blue for light microscopy
• Guy and Leake’s method
→ Uses crystal violet, sodium citrate, and formalin
→ Also used for light microscopy
• 1% ammonium oxalate diluting fluid for phase-contrast microscopy

72
Q

NEUBAUER COUNTING CHAMBER
• Total Area:
• Depth:
• Total volume:

A

• Total Area: 9 mm2
• Depth: 0.1 mm or 1/10 mm

• Total volume: 0.9 mm3 or 0.9 μL

73
Q

SPEIRS-LEVY COUNTING CHAMBER
• Total Area:
• Depth:
• Total Volume:

A

• Total Area: 10mm3
• Depth: 0.2mm
• Total Volume: 2.0mm3

74
Q

FUCHS-ROSENTHAL COUNTING CHAMBER
• Total Area:
• Depth:
• Total Volume:

A

• Total Area: 16mm2
• Depth: 0.2mm
• Total Volume: 3.2mm3

75
Q

3° TERTIARY SQUARE (SMALL SQ. ON THE CORNER) area

A

𝟎. 𝟎𝟔𝟐𝟓𝒎𝒎𝟐

76
Q

3° TERTIARY SQUARE (SMALL SQ. ON THE CENTER) area

A

𝟎. 𝟎𝟒𝒎𝒎𝟐

77
Q

4° QUATERNARY SQUARE (SMALLEST SQ.)

A

𝟎. 𝟎𝟎𝟐𝟓𝒎𝒎𝟐

78
Q

Rbc counting area

A

• Usually used is the 5 small squares on the central square
• There are 5 small squares and each square has an area of 0.04 mm2

79
Q

WBC counting area

A

• We count on 4 large corner squares and each square has an area of 1 mm2
→ What is the general area for WBC counting?
4 𝑥 1 𝑚𝑚2 = 𝟒 𝒎𝒎𝟐

80
Q

Platelet counting area

A

• We only use the entire large central square, which has an area of 1 mm2
• There are 25 small squares and each square has an area of 0.04 mm2
→ What is the general area for platelet counting?
25 𝑥 0.04 𝑚𝑚2 = 𝟏 𝒎𝒎𝟐