Hema 1 Part 1 Flashcards
Blood is ____________________ of the body weight
7.8%
Total blood volume of adult:
Male: _____________ Female: _____________
Male: 5-7 L Female: 4-6L
Composition of blood:
___________ = Formed elements (cells)
__________ = Fluid portion
45% = Formed elements (cells)
55% = Fluid portion
___________ = fluid portion of unclotted or anticoagulated blood; contains __________
Plasma, Fibrinogen
__________ = fluid portion of clotted blood
Serum
Plasma is made up of _____________ water and _____________ solutes
90% water
10% solutes
Skin Puncture
▪ Used for the following types of patients:
o Infants less than ______ of age
6 months
Skin Puncture
▪ Used for the following types of patients:
when veins cannot be used because of__________
IV infusions
Skin Puncture
▪ Used for the following types of patients:
o Infants less than 6 months of age
o Young children if only small amount of blood is needed
o Adults with poor veins
o when veins cannot be used because of IV infusions
o extreme obesity
o thrombotic tendencies
o point-of-care testing or with patients performing tests at home
Sites of Skin puncture
o Infants less than______ of age
1 year
Sites of Skin puncture
o Infants less than 1 year of age
•______ portion of the plantar surface of the foot
medial or lateral
Sites of Skin puncture
o Infants less than 1 year of age
• depth of the puncture should not be more than __________
2.0 mm
Sites of Skin puncture for Patients older than one year
Second, third or fourth finger (middle finger is the most commonly used
Sites of Skin puncture for Patients older than one year:
Puncture should be______ to the fingerprint, on the______ of the end portion of the finger, slightly off-center
perpendicular; palmar surface
Skin puncture site should be warmed at approximately __________
42 degrees
______ should be filled first followed by any other anticoagulant during blood collection
EDTA
Tournique should be approximately ________________ long and _________ wide
18-20 inch long and 1 inch wide
Tourniquet should be placed approximately ______________________ away from the
puncture site
3-4 inches
Tourniquet should not be left for more than _______ minute
1 min
If greater than one minute has elapsed, tourniquet should be removed and reapplied after ___________have elapsed
2 mins
Gauge number of the needle is ___________ proportional to the inside diameter of the needle (bore size)
Inversely
The higher the gauge number, the ____________ the diameter of the needle is.
The lower the gauge number, the ____________ the diameter of the needle is.
The higher the gauge number, the SMALLER the diameter of the needle is.
The lower the gauge number, the BIGGER the diameter of the needle is.
Gauge number found in needles attached to blood
bags: ___________
16 gauge
Most commonly used gauge number for routine
venipuncture: _______
21 gauge
Gauge number preferred for adult venipuncture:
__________________
19,20,21,22 (23 not routinely used)
Length of needle used for routine venipuncture:
_______________
1.0-1.5 inches
Pink needle hub
18
Brown/ivory needle hub
19
Yellow needle hub
20 G
Green needle hub
21 G
Black needle hub
22 G
Blue-green/ Blue needle hub
23 G
Red tube
Non-additive
Light blue tube
Sodium citrate
Gray tube
Sodium fluoride/Potassium oxalate
Green tube
Lithium or Sodium heparin
Yellow tube
SPS, Acid Citrate Dextrose (ACD)
Black tube
Sodium Citrate
Order of Draw for Evacuated Tubes and Syringe (Henry 22nd ed)
Blood culture tubes
Citrate
Serum
Heparin
EDTA
Sodium Flouride
Order of draw for skin puncture
Blood gases
Slide/ smear
EDTA
Other additive tubes
Serum tubes
Antiseptics used to clean the venipuncture site: ______________________
70% isopropyl alcohol
Antiseptics used to clean the venipuncture site: ______________________
70% isopropyl alcohol
Angle of the needle to the skin during venipuncture: _____________
15-30 degree
Degrees of-
Brachial Arterial Puncture:
Femoral Arterial Puncture:
Brachial: 45-60
Femoral: 90
Patients with IV lines: IV line should be stopped for _____________
________mL of blood should be collected and discarded
2 mins, 5 mL
Gentle inversions ______________
Atleast 8-10 times
A technologist or a student should not stick a patient more than _____times
2
Complications of Phlebotomy
Ecchymosis (bruise)
Hematoma
Syncope (fainting)
Hyperventilation
Edema
Thrombophlebitis
- EDTA (ethylenediaminetetraacetic acid)
▪ Most commonly used EDTA: _________________________________
Potassium
Spray-dried EDTA: _______________ Liquid EDTA: ____________________
K2, K3
EDTA (ethylenediaminetetraacetic acid)
Inhibits coagulation by _______________________
Chelsting calcium ions
Concentration of EDTA: ______________________
1.5 mg/mL of blood
Proper concentration of EDTA should be maintained:
Excess EDTA causes: _________________________
RBC shrinkage
Excess EDTA causes RBC shrinkage:
Hct becomes falsely _______________
ESR becomes falsely ______________
Both decrease
EDTA Preserves morphology of blood if used within ___________
2 hrs
It contains Acid mucopolysaccharide (mucoitin polysulfuric acid)
Heparin
Heparin Found as a naturally occurring anticoagulant produced by the _________
Liver
Heparin
Acts by inhibiting _________________Acts together with a cofactor ______________
Inhibit thrombin
Cofactor anti thrombin III
Heparin
Concentration: _____________________ Used for _____________
15-200 mL for EOFT
Not used for blood film preparation because it causes blue coloration of the background of blood films stained with Romanowsky stain
Heparin
Sodium Citrate
▪ Anticoagulant of choice for _______________________
Coagulation studies
Sodium Citrate Inhibits calcium by forming ______________________
Soluble complexes
In sodium citrate Potassium oxalate inhibits calcium by forming _________________________
Insoluble complex
Light blue sodium citrate concentration
3.2%
Light blue sodium citrate concentration
3.2%
Black sodium citrate concentration
3.8%
Sodium citrate
Anticoagulant to Blood Ratio in light blue
1:9
Sodium citrate
Anticoagulant to Blood Ratio in black
1:4
Light blue sodium citrate is for
Coagulation studies
Black sodium citrate is for
Erythrocyte
Sedimentation Rate
(ESR)
(Original Westergren)
Tube should be completely filled, underfilled tubes leads to falsely prolonged
PT and PTT
Sodium citrate is used if patient’s hematocrit does not exceed
0.55L/L
In patients with ______sodium citrate must be reduced
polycythemia vera (PV, Hct > 0.55L/L),
In patients with PV, PT and Aptt are falsely
prolonged
ERRORS IN VENIPUNCTURE PREPARATION (BEFORE PROCEDURE)
• Improper patient identification
• Failure to check patient adherence to dietary restrictions (Ex. Fasting)
• Failure to calm patient prior to blood collection
• Use of improper equipment and supplies
• Inappropriate method of collection
ERRORS IN VENIPUNCTURE PROCEDURE (DURING PROCEDURE)
• Failure to dry the site completely after cleansing with alcohol
• Inserting the needle bevel side down
• Use of needle that is too small, causing hemolysis of the specimen
• Venipuncture in an unacceptable area
• Prolonged tourniquet application
• Wrong order of tube draw
• Failure to mix blood collected in additive-containing tubes immediately
• Pulling back on syringe plunger too forcefully
• Failure to release tourniquet prior to needle withdrawal
ERRORS AFTER VENIPUNCTURE COMPLETION (AFTER PROCEDURE)
• Failure to apply pressure immediately to venipuncture site
• Vigorous shaking of anticoagulated blood specimen
• Forcing blood through a syringe needle into tube
• Mislabeling of tubes
• Failure to label appropriate specimens with infectious disease precaution
• Failure to put date, time, and initials on requisition
• Slow transport of specimens to laboratory