Hema 1 Part 2.1 Flashcards
HOWELL JOLLY BODIES seen in
→ Megaloblastic anemia
Heinz bodies seen in
Lead poisoning and G6PD
PAPPENHEIMER BODIES seen in
Sideroblastic anemia
BASOPHILIC STIPPLING seen in
Condition/s:
→ Lead poisoning / intoxication
→ Pyrimidine-5-nucleotidase
deficiency
CABOT RINGS seen in
Megaloblastic anemia
HEMOGLOBIN CRYSTALS seen
cells
→ Condition/s:
▪ Thalassemia
▪ Hemoglobinopathy
− FINDINGS: Target cells
+ Hgb SC
POLYCHROMASIA seen in
Retics
• Bi-lobed, showing pince-nez
appearance
• Hyposegmentation
PELGER-HUET ANOMALY
• May be seen with macro
ovalocytes around the red cells
Hypersegmentation
• Thickened, enlarged granules
ONLY in Neutrophils
→ Can be accompanied by
vacuolation
• Most common
Toxic granulation
GAUCHER CELL seen in
Glucocerebrosidase
deficiency or Gaucher Disease
FOAM CELL seen in
Niemann-Pick Disease
• Marginates / indent with the
surrounding RBCs
• The cell membrane adjusts to the
surrounding RBCs
ATYPICAL / VARIANT / REACTIVE LYMPHOCYTE / DOWNEY CELLS /
TURK’S IRRITATION CELL
Lymphocytes that are larger than
usual
SEZARY CELLS
Multinucleated giant cell with
OWL’S EYE appearance
REED-STERNBERG CELLS
REED-STERNBERG CELLS seen in
Hodgkin’s lymphoma
Supravital stains:
→ New methylene blue
→ Brilliant cresyl blue
→ Crystal violet
May be used to aid in counting reticulocytes
MILLER DISC
CORRECTED RETICULOCYTE COUNT
• Used to correct the reticulocyte counts in patients with
anemia
Hemoglobin iron content = _____of hemoglobin
0.347%
Hemoglobin that contains iron in the ferric stat
METHEMOGLOBIN
Will cause chocolate brown discoloration of blood
METHEMOGLOBIN
• Will cause CHERRY RED discoloration of blood
• has 200-245x more affinity to hemoglobin compared to O2
CARBOXYHEMOGLOBIN
Cannot bind or carry oxygen; reversible
CARBOXYHEMOGLOBIN
• Results when sulfur is incorporated in the heme rings of hemoglobin
• Causes MAUVE LAVENDER discoloration of blood
SULFHEMOGLOBIN
Cannot bind or carry oxygen; Irreversible; Not detected by the
cyanmethemoglobin method
SULFHEMOGLOBIN (SHB)
Also known as PACKED RED CELL VOLUMES
Hematocrit
Hematocrit
• Expressd in conventional unit:
• SI unit:
• Expressd in conventional unit: %
• SI unit: L/L ( liter per liter)
ADAM’S MICROHEMATOCRIT
Centrifugation speed and time
• Speed – 10,000 to 15,000 x gravity
• Time – 5 minutes
WINTROBE’S MACROHEMATOCRIT centrifugation
Centrifugation:
→ Speed: 2,000 – 2,300 x gravity (Barbara Brown)
→ Time: 30mins
Overanticoagulation of hematocrit mah lead to
Falsely decreased hematocri
Trapped plasma in hemtocrit may lead to
False increased hematocrit
→ Caused by poikilocytes
Length of westergren
300mm
Length of wintrobe
115 mm
Internal bore of westergren
2.65mm
External bore of westergren
5.5mm
Internal bore of wintrobe
3.0mm
Westergren graduation lines
200 mm
Graduation marks of wintrobe
100mm
Zeta sedimentation rate
• Special capillary:
→ ____long
→ ____ inner diameter
→ ____ outer diamete
→ 75mm long
→ 2.0mm inner diameter
→ 2.3mm outer diamete
Zeta sedimentation rate is filled _____with well mixed EDTA-anticoagulated
whole blood_______
is filled (3/4 full) with well mixed EDTA-anticoagulated
whole blood (approx. 0.2 ml)
AUTOMATED ESR SYSTEMS
• Include:
→ Ves-Matic
→ Mini-Ves
→ Sedimat
Automated ESR system TAT
Can give results in 15 to 20 minutes
