Specimen Collection & Handling Flashcards
Modified Allen Test
determine whether the ULNAR ARTERY can provide collateral circulation to the hand after the radial artery puncture
Best Site for blood gas analysis
Indwelling Umbilical Artery
Primary collection site for blood gas analysis
Radial Artery
Order of Draw for Capillary skin puncture
Blood Gas, Slide/Smears, EDTA, Other additive Microtainer, Serum Microtainer
Collection site for capillary puncture in individuals 1 year old and above
Finger stick (Middle or ring finger)
Order of Draw for Venipuncture
SPS/ACD, Sodium Citrate, No additive, Heparin, EDTA, Fluoride
Preferred vein for Venipuncture (M pattern)
Median Vein
Preferred vein for Venipuncture (H pattern)
Median Cubital
Tourniquet should be applied _____________ the site of collection and should not be longer than _______
Tourniquet should be applied 3-4 inches/7-10 cm the site of collection and should not be longer than 1 min/60 sec
Hemoconcentration affects what analytes?
It falsely increases: Enzymes, proteins, protein-bound substances, cholesterol, triglyceride, calcium, potassium
Blood pressure cuff can be used as tourniquet when inflated at _________
60 mmHg
A properly tied tourniquet has the ends pointing __________ and the loop pointing __________
A properly tied tourniquet has the ends pointing TOWARDS THE SHOULDER and the loop pointing TOWARDS THE HAND
Most commonly used needle length
1 in & 1.5 in (2.54 cm & 3.81 cm)
What length of needle provides better control for venipuncture
1 in
Most common gauge of needle used
g19, g20, g21
Needle should be inserted at what angle?
15 to 30
What is the appropriate course of action when collecting blood samples in patients with an IV
-Use the opposite arm or perform needle stick
-Have the nurse turn off the IV for 2 min
-Apply tourniquet below the IV
-Use a different vein if possible
How many inversions should be done on a light blue top ETS?
3-4
A Yellow top glass ETS is used for?
Molecular studies and cell culture
Most common complication in phlebotomy
Vascular complication
Appropriate course of action when collecting bloods in patients taking Streptokinase/Tissue plasminogen Activator
Hold pressure until bleeding has stopped
Second most common phlebotomy complication
Infections
Refers to the resting metabolic state of the body early in the morning after fasting
Basal State
Test status designations and priorities
STAT, Timed, ASAP, Fasting
Laboratory tests that require fasting
Gastrin, Aldosterone/Renin, Lipid Panel, Insulin, Triglyceride, Glucose Tolerance Test, Fasting Glucose
(GALIT GF ko kaya Fasting nanaman kami)
Laboratory tests that require chilling
Pyruvate, PTH, Ammonia, Gastrin, Glucagon, ACTH, Ammonia, Renin, Acetone Lactic Acid
(PPAGG AARAL)
Laboratory Test Samples that needs protection from light
Bilirubin, Carotene, Erythrocyte Protoporphyrin, Vitamin A, Vitamin B 12
Cortisol peaks at what time of day?
4 - 6 AM
What time of day is Cortisol lowest?
8 pm - 12 am
Cortisol is ___ lower at 8 pm that at 8 am
50%
Prolactin is higher during _____ and at _______
Prolactin is higher during 4 - 8 am and at 8 - 10 pm
Iron peaks at what time of day?
Early to late morning
Iron decreases up to ___ during the day
30%
Which laboratory tests are affected with day to day variation
CK, ALT, Bilirubin, Iron, TSH, TAG, Steroids
Alkaline phosphatase increases after ________
Alkaline phosphatase increases after a high-fat meal ingestion
