Specimen Collection & Handling

Question 1

Specimens are acceptable for up to ___ hours without transport media.

Answer 1

2

Question 2

Manner of collection of urine if patient cannot void

Answer 2

Straight catheterized

Question 3

How many specimens should a patient obtain for pulmonary tuberculosis diagnosis?

Answer 3

2 specimens in a span of 3 days

Question 4

Most preferred media for enhancement of stool pathogen recovery; inhibits normal flora

Answer 4

Enrichment media

Question 5

Ratio of stool to preservative

Answer 5

1:3

Question 6

Tubes used for gastric specimens

Answer 6

Levine (nose) & Rehfuss (mouth)

Question 7

Most preferred transport media for genital tract specimens (N. gonorrhoeae)

Answer 7

JEMBEC (James E. Martin Biological Environmental Chamber)

Question 8

What tube # is sent to Bacteriology for CSF?

Answer 8

2

Question 9

Why is it important that there be no delays in examining CSF?

Answer 9

Organisms associated with meningitis are prone to drying and chilling (fastidious)

Question 10

Volume required for CSF collection

Answer 10

1 mL

Question 11

Most preferred preservative for urine colony count

Answer 11

Boric acid

Question 12

Transport media used for stool (or enteric pathogens) if the delay is more than 2 hours

Answer 12

Cary Blair

Question 13

Indication of the presence of neutrophils

Answer 13

Inflammation

Question 14

Most bacteria and acid-fast bacilli grow at what temperature?

Answer 14

37C

Question 15

Most common anaerobic jars

Answer 15

GasPak, Brewer Jar, Glove Box, Biobag system

Question 16

The proper blood:broth ratio for blood cultures to reduce the antibacterial effect of serum in adults is:

Answer 16

1:10

Question 17

TRUE or FALSE: Adding 0.025-0.050% SPS will inhibit phagocytosis and complement that can cause a false-negative.

Answer 17

True

Question 18

Too much SPS can inhibit the growth of all strains of:

Answer 18

Peptostreptococcus anaerobius