Enterobacteriaceae Flashcards
All enterics are encapsulated except:
Klebsiella & Enterobacter
Yersinia is non-motile at ___C but motile at ___C
37; 25
Which of the enterics are the true pathogens? (not members of the normal flora)
Shigella, Salmonella, Yersinia
All enterics are catalase(+) except:
S. dysenteriae
All enterics are oxidase(-) except:
P. shigelloides
What are the rapid lactose fermenters?
E. coli, Klebsiella, Enterobacter
Also known as somatic antigen
O antigen
Common name of Escherichia coli
Colon bacillus
E. coli IMVC:
++–
Which E. coli virulence factor is responsible for iron chelation?
Aerobactin
Common cause of watery diarrhea among adults and children; Traveler’s diarrhea/Montezuma’s revenge
Enterotoxigenic E. coli (ETEC)
This heat-labile toxin stimulates adenylate cyclase which increases the conversion of ATP to Camp
Enterotoxin A
Camp accumulation leads to ____ of electrolytes and fluids into the intestinal lumen which results to watery diarrhea
Hypersecretion
Causative agent of infantile diarrhea
Enteropathogenic E. coli (EPEC)
Shigella-like E. coli; causative agent of bloody diarrhea
Enteroinvasive E. coli (EIEC)
Also known as Shiga-Toxin E. coli (STEC) or Verocytotoxic E. coli (VTEC)
Enterohemorrhagic E. coli (EHEC)
What is the most important strain of E. coli?
O157:H7
Phage-encoded cytotoxin identical to Shiga toxin
Verotoxin I
Culture media most useful in isolating EHEC (colorless colonies)
Sorbitol MacConkey Agar (SMAC)
E. coli which causes diarrhea by adhering to Hep2 cells and are packed in a “stacked brick” pattern
Enteroaggregative E. coli (EAEC)
E. coli may have the ability to cause meningitis among neonates if it possesses the ___ antigen.
K1
Klebsiella IMVC:
–++
TRUE or FALSE: Klebsiella grows in KCN broth.
True
Common name of Klebsiella pneumoniae
Friedlander’s Bacillus or Bacillus Capsulatus
Causative agent of destructive type of lobar pneumonia
Klebsiella pneumoniae
Currant, jelly-like sputum
K. pneumoniae
Only indole (+) organism under the genus Klebsiella
K. oxytoca
Causes atrophic rhinitis or ozena
K. pneumoniae subsp ozaenae
Isolated from patients with rhinoscleroma
K. pneumoniae subsp rhinosclermoatis
Tests which differentiate between Enterobacter and Klebsiella
Motility & ODC
Enterobacter IMVC:
–++
Only Enterbacter sp that is ADH (+)
E. cloacae
Only Enterobacter sp that is LDC (-)
E. cloacae & E. gergoviae
Causative agent of osteomyelitis after traumatic wounds
E. cancerogenus
Causative agent of meningitis ad bacteremia among neonates; yellow-pigmented colonies
Cronobacter sakazakii
Causative agent of septicemia due to contaminated IV fluids; triple-decarboxylases (-)
Panoea agglomerans
Opportunistic pathogens associated with outbreaks in healthcare settings
Serratia
All Serratia sp are slow lactose fermenters, except:
S. fonticola
Serratia IMVC:
–++
Hospital-acquired infections of the urinary or respiratory tract; bacteremic outbreaks in nurseries, cardiac surgery, burn units
S. marcescens
S. odorifera odor:
Rotten potato
Linked to HUS, GvHD, sepsis
Hafnia alvei
Characteristic struvite kidney stones (urease activity)
Proteus
Term for Proteus vegetative cells
Swimmers
Proteus odor:
Burnt chocolate (gun powder)
Providencia IMVC:
++-+
Causative agent of outbreaks in burn units
Providencia stuartii
Edwardsiella IMVC:
++–
Test that distinguishes E. tarda with E. coli
H2S
Citrobacter IMVC:
-+-+
Salmonella IMVC:
-+-+
Non-typhoidal fever with or without gastroenteritis
Salmonella serotype choleraesuis
TRUE or FALSE: S. typhi & S. paratyphi have animal reservoirs.
False
S. typhi virulence antigen; capsular, anti-phagocytic
Vi antigen
Infective dose of S. typhimurium
10^6
Specimen of choice during the first week of S. typhi infection:
Blood
Characteristic scybalous stool
S. typhi
____ week of S. typhi infection: rose spots, necrotizing cholecystitis
2nd-3rd
Enteric non-motile at 37C and at 25C
Yersinia pestis
Y. pseudotuberculosis & Y. enterocolitica are motile at ___C.
25C
Y. pestis has a ____ appearance due to bipolar bodies (with Wayson’s stain).
Safety pin
Yersinia IMVC:
-+–
Most important virulence factor of Y. pestis
Fraction 1
Most common form of plague
Bubonic
Form of plague where organisms proliferate in the bloodstream and the respiratory tract
Pneumonic
Y. pestis colonial morphology:
Fried egg colonies
TRUE or FALSE: Y. enterocolitica survives at cold temperature (4C).
True
Culture media used for Y. enterocolitica
Cefsulodin Irgasan Novobiocin (CIN)
Y. enterocolitica colonial morphology on CIN:
Bull’s eye colonies (red centers due to mannitol fermentation)
Caseous swellings that are fatal in animals
Pseudotubercles
Y. pseudotuberculosis is motile at ___C.
18-22C
Organism acquired through ingestion of undercooked shellfish
Plesiomonas shigelloides