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Bacteriology > Antimicrobial Agents & AST > Flashcards

Antimicrobial Agents & AST Flashcards

1
Q

Inhibits transport of lipid-bound precursors across the cytoplasmic membrane; from Bacillus licheniformis

A

Bacitracin (Taxo A)

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2
Q

Act like detergents which interact with phospholipids, increasing permeability

A

Polymyxins

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3
Q

Bacteria that possess OmpF and OmpC which decreases the permeability of B-lactams

A

E. coli

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4
Q

Gene possessed by MRSA which encodes for PBP (B-lactamase-resistant transpeptidase) with a low affinity for B-lactams

A

mecA

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5
Q

Gene possessed by S. agalactiae against macrolides

A

mreA

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6
Q

Agar used for Kirby Bauer Disk Diffusion Method

A

Muller-Hinton Agar

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7
Q

Aid for visualization between broth and McFarland standard

A

Wickerman card

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8
Q

Automated inspection of turbidity

A

Nephelometer or spectrophotometer

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9
Q

What does a large ZOI mean?

A

Effective antibiotic (susceptible)

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10
Q

Standard disk width

A

6 mm

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11
Q

Diameter of MHA used

A

150 mm

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12
Q

How many disks can the plate accommodate?

A

12

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13
Q

Distance between antibiotic disks to prevent overlapping

A

> 24 mm

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14
Q

What are the temperature and time for incubation?

A

35C for 16-18 hours (5-10% for fastidious organisms)

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15
Q

Broth used for broth dilution

A

Mueller-Hinton broth

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16
Q

Testing 1 or 2 antibiotics with the use of 13 test tubes (1-2 mL)

A

Macrodilution

17
Q

What tube # will be the inoculum control in macrodilution?

A

Tube 11

18
Q

What tube # will be the antibiotic control in macrodilution?

A

Tube 13

19
Q

Standard inoculum for broth dilution

A

5x10^5 CFU/mL

20
Q

Standard inoculum for agar dilution

A

1x10^4 CFU/mL

21
Q

What is the lowest concentration of an antimicrobial agent in agar that completely inhibits visible growth?

A

Minimum inhibitory concentration (MIC)

22
Q

Standard inoculum for disk diffusion

A

1.5x10^8 CFU/mL

23
Q

What happens if the agar depth is <3mm?

A

False susceptibility

24
Q

What happens if the age of inoculum exceeds 24 hours?

A

False susceptibility

25
Q

Test for fastidious organisms where thin plastic strips (with gradations) containing antibiotics are placed in a radial fashion on an inoculated plate

A

Episolemeter test (E-test)

26
Q

What would be the MIC on CHROM agar if there is presence of MRSA?

A

> 4 ug/mL

27
Q

Measures the rate of killing pf bacteria by measuring the number of remaining viable bacteria

A

Time kill assay

28
Q

Measures susceptibility to a combination of antimicrobial agents

A

Synergy

29
Q

What antimicrobial level is the therapeutic indicator?

A

Peak level (15-20 minutes after dose is administered)

Bacteriology (11 decks)

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