Specimen Collection and Processing Flashcards
any contamination of the blood with tissue fluid leads to
incorrect results
for routine coagulation procedures, the ___ system is adequate
vacutainer blood collection system
if only coagulation tests are ordered, a plain tube should be filled with
2 to 3 ml of blood
when obtaining a blood specimen for special coagulation procedures, a two syringe technique may be applied using?
plastic syringes
are generally not used for coagulation
capillary specimens
all test tubes used for coagulation studies should have a ____ surface
noncontact surface
it is recommended to use ___ and not glass or soda lime
siliconized surface
the anticoagulant of choice for coagulation studies is ____
0.109 M sodium citrate
alternate for 0.109 sodium citrate, but is not the recommended concentration
0.129 M sodium citrate
the ratio of blood to anticoagulant
9 parts blood to 1 part sodium citrate
blood specimens with a high hematocrit will contain ___ in relation to the volume of blood in the tube
less plasma
the coagulation specimen should be centrifuged within _ of obtaining the sample
1 hour
it is desirable to complete testing within ___ hours depending on the specific procedure
2-4 hours
all pipets, test tubes, and reaction cups which come in contact with test plasma should have a
noncontact surface
the plasma specimen should be in a stoppered container, this prevents?
loss of CO2 and resultant pH change of plasma
for coagulation procedures, specimen will be tested within 2 hours of collection may be kept at?
room temperature
if there is a delay beyond 2 hours it is advisable to
keep specimen on ice
exceptions for the rule (> hour kept on ice), should be maintained at room temp
specimens for prothrombin time, factor VII assay, and platelet function studies
tested or frozen within 30 minutes of collection
plasma for euglobin clot lysis
with the exception of platelet function studies, plasma for coagulation testing should be
platelet poor
poor plasma platelet count
less than 15,000 ul
will routinely produce acceptable platelet poor plasma
15 minutes at 2500 x g centrifugation
- contains an aqueous solution within a sealed unit, available in various sizes
- alternative method to crushed ice
kryorack
kyrorack when placed in a freezer temperatures (-18C) for 8 hours, it maintains a temperature of
less than 8c for 8 hours at room temp
is indicative of a traumatic venipuncture
hemolyzed plasma
