SPECIMEN COLLECTION Flashcards
IT AFFECTS THE VOLUME SHIFTS BETWEEN VASCULAR AND INTERSTITIAL COMPARTMENTS
EXERCISE
IT IS INCREASED DURING EXERCISE
GROWTH HORMONE PROLACTIN TESTOSTERONE LEUTENIZING HORMONE LACTATE FATTY ACID AMMONIA ALT AST CPK LD
ELEVATED IN FIST CLENCHING
POTASSIUM
LACTATE
PHOSPHATE
REQUIRED FASTING FOR GLUCOSE, LIPID, LIPOPROTEINS
8-16 HOURS
IT INCREASES AFTER 48 HOURS OF FASTING
SERUM BILIRUBIN
IT INCREASES AFTER 2 HOURS OF FASTING
PLASMA TAG
BASAL STATE COLLECTION
GLUCOSE
CHOLESTEROL
TAG
ELECTROLYTES
IT INCREASES UREA
HIGH PROTEIN DIET
MAY SHOW VARIATIONS DUE TO POSTABSORPTIVE HORMONAL EFFECTS
GLUCOSE, LIPIDS, AND CATECHOLAMINES
SEROTONIN INCREASES IN URINE EXCRETION OF
5-HYDROXYINDOKE ACETIC ACID
IT INCREASES CONCENTRATION OF GLUCOSE
CAFFEINE
WHAT DO CAFFEINE RELEASES
CATECHOLAMINE
TAG LEVELS THAT INCREASES TURBIDITY OR LACTESCENCE
400 MG/DL
BILIRUBIN LEVEL THAT COTRIBUTES TO ICTERIA
25.2 MG/L
WHAT DO ICTERIC SAMPLES INTERFERE
ALBUMIN
CHOLESTEROL
GLUCOSE
TOTAL PROTEIN
PREFFERED POSITION IN SPECIMEN COLLECTION
SUPINE OR UPRIGHT
AT LEAST HOW MINUTES DO PATIENT NEED TO BE IN SUPINE POSITION BEFORE COLLECTION
20 MINUTES
CONSTRICTION OF BLOOD AND REDUCE PLASMA VOLUME
SUPINE TO SITTING OR STANDING
INCREASED LEVELS OF ALBUMIN, ENZYMES AND CALCIUM
SUPINE TO STANDING OR SITTING
CAUSES SHIFTING OF WATER AND ELECTROLYTES INTO TISSUE CAUSING HEMOCONCENTRATION
SITTING TO SUPINE
INCREASED LEVELS OF PROTEINS, LIPIDS, BUN, IRON AND CALCIUM
SITTING TO SUPINE
CAUSES EXTRAVASCULAR WATER TO TRANSFER TO THE VASCUALR SYSTEM AND DILUTES NON DISSULFIDE PLASMA CONSTITUENTS
STANDING TO SUPINE
DECREASED LEVELS OF CHOLESTEROL, TAG AND LIPOPROTEINS
STANDING TO SUPINE
ITS SIGNIFICANT ELEVATION AFTER 30 MINUTES OF STANDING
POTASSIUM
RESULTS TO DECREASED PLASMA ALBUMIN DUE TO FLUID RETENTION
PROLONGED BED REST
INCREASED IN PROLONGED TOURNIQUET APPLICATION
PROTEINS (ALBUMIN), ENZYMES, LACTATE, CHOLESTEROL, POTASSIUM, AMMONIA
INCREASED IN NEFA CONCENTRATION
TOBACCO SMOKING
INCEASED IN CHOLESTEROL, UREA, TAG, GROWTH HORMONE, GLUCOSE, CATECHOLAMINES, CORTISOL
TOBACCO SMOKING
INCREASED:
GGT
URATE
TAG
ALCOHOLISM
CAUSES HYPOGLYCEMIA
ALCOHOLISM
IT AFFECTS ADRENAL HORMONE SECRETION
STRESS
INCREASED LEVELS OF ALBUMIN, GLUCOSE, INSULIN, LACTATE, AND CHOLESTEROL
STRESS
SPECIMEN COLLECTION SHOULD BE SCHEDULED ACCORDING TO TIME OF LAST DOSE
TDM
CAN DECREASED PLASMA SODIUM AND POTASSIUM LEVELS
DIURETICS
WHOLE BLOOD IS COMPOSED OF
60% PLASMA; 40% CELLS
FROM PATIENT’S ARTERY
OXYGENATED BLOOD
WITHOUT TOURNIQUET
ARTERIAL PUNCTURE
PREFERRED SPECIMEN FOR BLOOD GAS AND BLOOD PH
ARTERIAL PUNCTURE
SITES FOR ARTERIAL PUNCTURE
RADIAL BRACHIAL FEMORAL SCALP UMBILICAL
SITES FOR VENIPUNCTURE
ANTECUBITAL FOSSA
WRIST AND DORSAL OF HANDS
ANKLE
IT SHOULD BE USED ONLY IF ARM VEIN ARE UNSITABLE
ANKLE VEIN
ORDER OF DRAW
BLOOD CULTURE LIGHT BLUE SERUM TUBE GREEN LAVENDER GRAY
BEVEL UP ANGLE
15-30 DEGREES
BLOOD PRESSURE CUFF IS MAINTAINED AT
60 MMHG
IT IS USED FOR ETHANOL TESTING FOR CLEANSING
BENZALKONIUM (ZEPHIRAN CHLORIDE 1:750)
RELATIONSHIP OF GAUGE AND SIZE OF NEEDLE
INVERSELY PROPORTIONAL
NEEDLE LENGTH
1-1.5 INCH
IT INDICATES GAUGE OF NEEDLE
COLOR
TRAUMATIC DRAW CAN INCREASE
CK
MYOGLOBIN
POTASSIUM
THE INITIAL SAMPLE TO BE DISCARDED IN IV THERAPY
5ML
IT WILL INCREASE GLUCOSE IN >500MG/DL
10% CONTAMINATION
5% DEXTROSE
IT IS COLLECTED AFTER 3 DAY DIET FROM PERIPHERAL VEIN
RENIN BLOOD LEVEL
BASAL STATE COLLECTION REQUIREMENT
EARLY MORNING
12 HOURS FASTING
IT IS AN INCREASE IN NUMBER OF FORMED ELEMENTS IN BLOOD
HEMOCONCENTRATION
CAUSES OF FAILURE OF BLOOD TO ENTER
EXCESSIVE PULL TO PLUNGER PIERCING OTHER VEIN TRANSFIXATION OF VEIN INCORRECT BEVEL ABSENCE OF VACUUM
TRANSIENT LOSS OF CONSCIOUSNESS DUE TO LACK OF OXYGEN IN THE BRAIN
SYNCOPE (FAINTING)
IMMEDIATE CTION AFTER FAINTING
REMOVE NEEDLE IMMEDIATELY
LOWER HEAD BETWEEN LEGS
DEEP BREATHLY
ABNORMAL VASCULAR CONDITION THROMBUS DEVELOPS WITHIN BLOOD VESSEL
THROMBOSIS
INFLAMMATION OF VEIN ACCOMPANIED BY CLOT
THROMBOPLEBITIS
PREFFERED LENGTH OF LANCET
1.75MM
DEPTH OF INCISION FOR INFANTS AND ADULTS
<2.0 AND <2.5 MM
SKIN TO BONE DISTANCE IN SKIN PUNCTURE
1.5-2.4MM
PREFFERED SITE FOR INFANTS
LATERAL SURFACE OF TEH FOOT
SITES NOT PREFFERED FOR SKIN PUNCTURE
CENTRAL ARCH OF HEEL FINGERS OF <1 YEAR OLD THUMB INDEX AND 5TH FINGERS FINGERS ON SIDE OF MASECTOMY
ORDER OF FILLING MICROCOLLECTION TUBES
EDTA
ADDITIVES
NON-ADDITIVES
USED FOR BLOOD GAS ANALYSIS, PH, PCO2
ARTERIALIZED CAPILLARY BLOOD
PREFFERED SITE FOR ARTERIALIZED CAPILLARY BLOOD
EARLOBE
WHY IS EARLOBE A PREFFERED SITE FOR ACB
VASCULARITY
LOW METABOLIC REQUIREMENTS
EASE
MOST COMMONLY USED SITE FOR ACB
LATERAL PLANTAR HEEL SURFACE
WATER TEMPERATURE FOR ACB
39-45C
TUBES USED FOR ACB
2 HEPARINIZED TUBE
CAPILLARY ARTERIALIZATION SHOULD NOT BE DONE IF
BP <95MMHG
IT IS A MINUTE METAL FILLING INSERTED BEFORE COLLECTING BLOOD TO HELP MIX THE SPECIMEN WHILE BLOOD IS ENTERING THE TUBE
FLEA
USED TO DRAW FLEA BACK AND FORTH
MAGNET
BEST SITE FOR BLOOD GAS ANALYSIS FOR NEWBORN
INDWELLING UMBILICAL ARTERY
SEPARATION TIME OF PLASMA AND SERUM
1 HOUR
ALLOWABLE CLOTTING TIME
30 MINUTES
CENTRIFUGATION REQUIREMENT FOR BLOOD
1000-3000 RCF FOR 10 MINUTES
PREVENTS GLYCOLYTIC ENZYMES
2MG NAF/ML OF BLOOD
IT IS NOT NECESSARY IF PLASMA AND SERUM ARE SEPARATED
FLUORIDE TUBE
BILIRUBIN IS UNSTABLE AFTER
30 MINUTES
ENZYMES THAT WILL INCREASE DURING HEMOLYSIS
ACP, ALT, AST, LD
ELECTROLYTES THAT WILL INCREASE IN HEMOLYSIS
MAGNESIUM, POTASSIUM, PHOSPHORUS
20 MG/DL OF HEMOGLOBIN INDICATES
VIASUAL HEMOLYSIS
5% HEMOLYSIS WILL INDICATE
ELEVATED POTASSIUM (98-99% OF K IN RBC)
SHOULD BE AVOIDED IN TUBE THAT MAY CAUSE HEMOLYSIS AND AEROSOL INFECTION
RIMMING
CAN CAUSE SLIGHT DILUTIONAL EFFECT ON LOWER CONCENTRATION IN RBC THAN PLASMA
SEVERE HEMOLYSIS
EXCESSIVE CENTRIFUGATION MAY CAUSE
LYSIS
SLIGHT ELEVATION OF LD AND POTASSIUM
INCREASE IN PLASMA PROTEINS, BILIRUBIN, LD, AND POTASSIUM
DECREASE IN SODIUM AND CHLORIDE
WHOLE BLOOD OR PLASMA TRANSFUSION
ARE AFFECTED BY EVAPORATION
ELECTROLYTES
MAY CAUSE DISRUPTION TO STRUCTURE OF LARGE PROTEIN
ICE CRYSTALS
IT MEASURES TEH SPEED OF CENTRIFUGATION
TACHOMETER
AFFECTED BY LOW TEMPERATURE STORAGE
DECREASED LDH 4 AND 5
INCREASE ALP
SPECIMENS TAHT REQUIRE CHILLING
AMMONIA, BLOOD GAS, CATECHOLAMINE, GASTRIN, LACTIC ACID, RENIN, PTH, PYRUVATE
PHOTOSENSITIVE ANALYTES
BILIRUBIN, BETA CAROTENE, FOLATE, PORPHYRINS, VITAMINS A AND B6
IT WILL BECOME CARBINATE IF HIGHER THAN 8
OXALATE
COMBINES WITH CALCIUM TO FORM INSOLUBLE SALT
OXALATE
INTERFERES WITH SODIUM, POTASSIUM AND BUN
OXALATE
FLUORIDE
COMBINES WITH CALCIUM IN NON IONIZED FORM
CITRATE
IT USES IN CEA, TDM AND LEAD TESTING
EDTA
DISODIUM FORM, CRYSTAL
VERSENE
DIPOTASSIUM, LIQUID
SEQUESTRENE
HAS ANTIGLYCOLYTIC AGENT
FLUORIDE
IDEAL UNIVERSAL ANTICOAGULANT
FACTOR X
HEPARIN AVAILABLE AT
SODIUM, LITHIUM, POTASSIUM AND AMMONIUM SALTS
IT IS USED FOR GLUCOSE, BUN, IONIZED CALCIUM, ELECTROLYTE STUDIES AND CREATININE
LITHIUM HEPARIN
NUMBER 1 ENZYME ACTIVATOR
CALCIUM
ANTICOAGULANT FOR POTASSIUM
HEPARINIZED PLASMA
CAN ALSO BE USED FOR LACTATE AND ALCOHOL DETERMINATION
GRAY TOP
HAS SPECIFIC GRAVITY BETWEEN BLOOD CLOT AND SERUM
MOVES UPWARD TO SERUM-CLOT INTERFACE
POLYMER BARRIER
RESPINNING OF GEL TUBE CAUSES
INCREASE POTASSIUM
PREFFERED ANTICOAGULANTS FOR MICROCOLLECTION TUBES
LITHIUM AND AMMONIUM SALTS OF HEPARIN
