specimen col and handling Flashcards
concentration on skin scrapings, soft tissues, body fluids
KOH 10%
Prevents drying of specimen
KOH with Glycerol
concentration on nails, hair, hard specimen
KOH 20%
Enhances action of KOH and Hastens the activity of KOH
14% Dimethyl Sulfoxide (DMSO)
Green fluorescence
KOH with Calcofluor white
preserve sample
Lactic acid
kills bacteria/inactivate biological hazards
phenol
silver nitrate outlines
fungi
Methenamine
Silver Stain
staining of lactophenol blue
cotton blue
polysaccharide in
the cell walls
Periodic-Acid
Schiff Stain
Fungi stain pink-red with blue nuclei
against green background
Periodic-Acid
Schiff Stain
For rapid staining of blood and bone
marrow fungi
Wright’s/ Giemsa
Stain (Diff quick)
Used to differentiate the acid-fast Nocardia
from other aerobic Actinomyces
Acid Fast Stain (Kinyoun modification)
Fungi cell wall
precipitates silver
Methenamine
Silver Stain
useful in identifying Capsule-Deficient
Cryptococcus neoformans and
typical Cryptococcus neoformans
Masson Fontana
Best stain for fungi
in tissue
Methenamine
Silver Stain
it stain dark blue or
rose in gridley stain
Hyphae and yeast
The internal parts of
hyphae are deep
rose to black, and
the background
is light green.
Methenamine
Silver Stain
it stain deep blue and background is
yellow in gridley
tissues
Stains the melanin of the cell wall
brown against a pink-purple background
Masson Fontana
Fungi stains pink to
pinkish blue
Hematoxylin and
Eosin Stain
using Acridine Orange fungal elements emit
green fluoroscence
Cryptococcus neoformans deep
rose
Mucicarmine
Stain (Mayer’s or
Southgate)
non Acid Fast Stain color
bluish
routine stain for histopathology
Hematoxylin and
Eosin Stain
using Acridine Orange epithelial cells are
orange
Acid Fast Stain color
fuschia
Initial
differentiation of
dimorphic fungi
and sputum
smears also
Papanicolaou
Stain
Papanicolaou Stain fungal elements color
pink
