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MYCOVIR > specimen col and handling > Flashcards

specimen col and handling Flashcards

1
Q

concentration on skin scrapings, soft tissues, body fluids

A

KOH 10%

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2
Q

Prevents drying of specimen

A

KOH with Glycerol

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2
Q

concentration on nails, hair, hard specimen

A

KOH 20%

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2
Q

Enhances action of KOH and Hastens the activity of KOH

A

14% Dimethyl Sulfoxide (DMSO)

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3
Q

Green fluorescence

A

KOH with Calcofluor white

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4
Q

preserve sample

A

Lactic acid

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5
Q

kills bacteria/inactivate biological hazards

A

phenol

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5
Q

silver nitrate outlines
fungi

A

Methenamine
Silver Stain

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6
Q

staining of lactophenol blue

A

cotton blue

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6
Q

polysaccharide in
the cell walls

A

Periodic-Acid
Schiff Stain

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6
Q

Fungi stain pink-red with blue nuclei
against green background

A

Periodic-Acid
Schiff Stain

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6
Q

For rapid staining of blood and bone
marrow fungi

A

Wright’s/ Giemsa
Stain (Diff quick)

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7
Q

Used to differentiate the acid-fast Nocardia
from other aerobic Actinomyces

A

Acid Fast Stain (Kinyoun modification)

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7
Q

Fungi cell wall
precipitates silver

A

Methenamine
Silver Stain

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7
Q

useful in identifying Capsule-Deficient
Cryptococcus neoformans and
typical Cryptococcus neoformans

A

Masson Fontana

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7
Q

Best stain for fungi
in tissue

A

Methenamine
Silver Stain

7
Q

it stain dark blue or
rose in gridley stain

A

Hyphae and yeast

8
Q

The internal parts of
hyphae are deep
rose to black, and
the background
is light green.

A

Methenamine
Silver Stain

8
Q

it stain deep blue and background is
yellow in gridley

A

tissues

8
Q

Stains the melanin of the cell wall
brown against a pink-purple background

A

Masson Fontana

8
Q

Fungi stains pink to
pinkish blue

A

Hematoxylin and
Eosin Stain

8
Q

using Acridine Orange fungal elements emit

A

green fluoroscence

8
Q

Cryptococcus neoformans deep
rose

A

Mucicarmine
Stain (Mayer’s or
Southgate)

8
Q

non Acid Fast Stain color

A

bluish

8
Q

routine stain for histopathology

A

Hematoxylin and
Eosin Stain

9
Q

using Acridine Orange epithelial cells are

A

orange

9
Q

Acid Fast Stain color

A

fuschia

10
Q

Initial
differentiation of
dimorphic fungi
and sputum
smears also

A

Papanicolaou
Stain

11
Q

Papanicolaou Stain fungal elements color

A

pink

MYCOVIR (4 decks)

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