fungcul Flashcards
Most MOLDS grow best at
25-30°C
Most YEAST grow best at
35-37°C
causes rose gardener’s
disease
Sporothrix schenckii
Most pathogenic fungi grow best:
30 to 32°C
Sporothrix schencki temp
25 to 27°C than 30°C
Once an individual is thorned that contains
Sporothrix schenckii, it will cause
cutaneous mycoses
general incubation period
14 days
incubation period respiratory, bone marrow, blood specimens, and specimens in which dimorphic fungus are suspected
28 days
For differentiation of C. dubliniensis versus Candida albicans
Birdseed Agar (Niger Seed Agar; Staib Agar)
to detect presence of yeast in the mouth,
throat, or vagina (cytologic specimen: cells) incubation period
7 days:
incubation period tissues and sterile body fluids other than
blood
21 days
what are the General purpose media
(a) Sabouraud dextrose
(b) malt extract and seldom is
(c) brain heart infusion medium
antibiotic added to prevent the growth of
bacteria
Chloramphenicol
Recommended for slide cultures
Potato Dextrose Agar
antifungal added to reduce the growth of
environmental fungi
Cycloheximide
recommended for the cultivation of fastidious
pathogenic fungi, such as Histoplasma capsulatum and Blastomyces dermatitidis
Brain Heart Infusion (BHI) Agar
primary medium for clinically encountered fungi and used for inducing an isolate to exhibit a characteristic
pigment and conidia.
Potato Dextrose Agar
For pigment production by Cryptococcus neoformans and Cryptococcus gattii
Birdseed Agar (Niger Seed Agar; Staib Agar)
medium for primary isolation of fungi directly from specimens
Sabouraud dextrose agar (SDA)
good medium for fungal detection, isolation and enumeration on yeast and mold
Malt Extract agar
used in distinguishing the different genera of yeast and the various species of Candida
Corn Meal Agar with Tween 80
red indicator of DTM
Phenol red solution
Combination of SDA and BHI
SABHI Agar
Dermatophytes change the color of the medium from
yellow to red within 14 days.
Isolation and definitive differentiation of the most common encountered yeasts.
CHROMagar Candida Medium
appear as purple fuzzy colonies.
krusei
Rapid growers growth rate
24 hours
colonies appear as cream to white
smooth colonies
glabrata
Most appropriate culture medium for Candida
CHROMagar
appear as blue to metallic blue
colored raised colonies.
tropicalis
appear as light green colored smooth
colonies
albicans
Detection of Trichosporon and Trichophyton spp.
Urea Agar
Detection of Microsporum audouinii and M. canis
Rice medium
Lesser disruption of colony compared to tease mount
Cellophane Tape
Dimorphic fungi growth rate
= 1-4 weeks
The colonies are disturbed from their original
position but a rapid method for identification of conidia and spores.
Tease Mount
agar that Form malassezia
Dixon agar
Dematiaceous fungi growth rate
1-5 days
Growth requirements (macroscopic)
* vary stain to stain however generally requires sources of
Carbon, Nitrogen and Vitamins.
the worm that migrates at night to the anal area to lay its egg
Enterobius vermicularis
Screening test which is used to differentiate Candida albicans from other yeast
Germ Tube Test
aka seat worm or human pinworm
Enterobius vermicularis
The best method for preserving and observing the actual structure of a fungus
Slide culture
Differentiate Trichophyton spp.
Hair perforation test
(+) urease on
Cryptococcus, Rhodotorulla,
Trichosporon spp., Trichophyton mentagrophytes
(-) urease
albicans
example of dimorphic
Histoplasma capsulatum,
Blastomyces dermatitidis, Paracoccidioides brasiliensis
example of dematiaceous fungi
black yeast
