Special Histochemical Stains for Microorganisms

Question 1

What microorganism(s) is/are best stained with Giemsa?

Answer 1

Metachromatic granules are stained, so it’s a good stain for protozoa & some bacteria.

Question 2

What microorganism(s) is/are best stained with Gram?

Answer 2

It’s the standard staining for bacteria.

Question 3

What microorganism(s) is/are best stained with Grocott methenamine silver?

Answer 3

fungi, Pneumocystis

Question 4

What microorganism(s) is/are best stained with Jimenez?

Answer 4

Chlamydiae

Question 5

What microorganism(s) is/are best stained with Macchiavello?

Answer 5

Chlamydiae

Question 6

What microorganism(s) is/are best stained with Mucicarmine?

Answer 6

capsule of Cryptococcus

Question 7

What microorganism(s) is/are best stained with Periodic Acid-Schiff (PAS)?

Answer 7

fungi

Question 8

What microorganism(s) is/are best stained with Steiner & Steiner silver?

Answer 8

Metachromatic granules are stained, so it’s a good stain for protozoa & some bacteria.

Question 9

What microorganism(s) is/are best stained with Toluidine blue?

Answer 9

metachromatic granules

Question 10

What microorganism(s) is/are best stained with Wade-Fite?

Answer 10

acid-fast bacteria including mycobacteria, Nocardia

Question 11

What microorganism(s) is/are best stained with Warthin-Starry?

Answer 11

Metachromatic granules are stained, so it’s a good stain for protozoa & some bacteria.

Question 12

What microorganism(s) is/are best stained with Ziehl-Neelsen?

Answer 12

acid-fast bacteria; Nocardia difficult to detect