Special Histochemical Stains for Intracellular & Extracellular Substances

Question 1

What substance/structure is stained by acid phosphatase? What color does it stain?

Answer 1

• prostate

- black

Question 2

What substance/structure is stained by Alcian blue? What color does it stain?

Answer 2

• sialomucins, hyaluronic acid, sulfated mucosubstances

- blue

Question 3

What substance/structure is stained by Alizarin red S? What color does it stain?

Answer 3

• calcium

- orange-red

Question 4

What substance/structure is stained by Best’s carmine? What color does it stain?

Answer 4

• glycogen

- deep red

Question 5

What substance/structure is stained by Bielschowsky’s silver stain? What color does it stain?

Answer 5

• axons

- black

Question 6

What substance/structure is stained by Congo red? What color does it stain?

Answer 6

• amyloid

- orange-red

Question 7

What substance/structure is stained by Cresyl violet? What color does it stain?

Answer 7

• Nissl substance

- violet

Question 8

What substance/structure is stained by Dunn-Thompson? What color does it stain?

Answer 8

• hemoglobin

- emerald green

Question 9

What substance/structure is stained by Feulgen? What color does it stain?

Answer 9

• DNA

- red-purple

Question 10

What substance/structure is stained by Fontana-Masson? What color does it stain?

Answer 10

• melanin

- black

Question 11

What substance/structure is stained by Gordon & Sweets Reticulin Fiber? What color does it stain?

Answer 11

• reticulin fibers

- black

Question 12

What substance/structure is stained by Grimelius? What color does it stain?

Answer 12

• argyrophilic granules

- black

Question 13

What substance/structure is stained by Hall’s? What color does it stain?

Answer 13

• bile/biliverdin

- green

Question 14

What substance/structure is stained by Jone’s Methenamine? What color does it stain?

Answer 14

• basement membranes

- black

Question 15

What substance/structure is stained by Kinyoun’s (modified Ziehl-Neelsen)? What color does it stain?

Answer 15

• lipofuscin

- red

Question 16

What substance/structure is stained by Luxol Fast Blue? What color does it stain?

Answer 16

• myelin

- blue

Question 17

What substance/structure is stained by Mallory’s PTAH? What color does it stain?

Answer 17

• muscle, fibrin, glial processes

- dark blue

Question 18

What substance/structure is stained by Masson’s Trichrome? What color does it stain?

Answer 18

• muscle, collagen

- muscle: red; collagen: blue

Question 19

What substance/structure is stained by Mayer’s Mucicarmine? What color does it stain?

Answer 19

• mucin, hyaluronic acid, chondroitin sulfate

- rose to red

Question 20

What substance/structure is stained by Methyl Green Pyronin? What color does it stain?

Answer 20

• nucleic acids

- DNA: green-blue; RNA: red

Question 21

What substance/structure is stained by Oil Red O? What color does it stain?

Answer 21

• fat

- orange-bright red

Question 22

What substance/structure is stained by Periodic Acid-Schiff (PAS)? What color does it stain?

Answer 22

• glycogen, mucin

- red

Question 23

What substance/structure is stained by Prussian Blue? What color does it stain?

Answer 23

• iron

- blue

Question 24

What substance/structure is stained by Rubeanic Acid? What color does it stain?

Answer 24

• copper

- green

Question 25

What substance/structure is stained by Schmorl’s reaction? What color does it stain?

Answer 25

• melanin, lipofuscin

- dark blue

Question 26

What substance/structure is stained by Sudan Black B? What color does it stain?

Answer 26

• fat

- black

Question 27

What substance/structure is stained by Toloudine Blue? What color does it stain?

Answer 27

• mast cells

- purple

Question 28

What substance/structure is stained by Verhoeff? What color does it stain?

Answer 28

• elastic fibers

- black

Question 29

What substance/structure is stained by Von Kossa? What color does it stain?

Answer 29

• calcium

- black