something Flashcards
what are the two laws of thermodynamics?
1-energy cannot be created or destroyed(tranformed)
2-closed energy(nothing coming in or out) will increase is entropy(disorder) over time due to energy transformation
what are the two forms of energy?
potential energy and kinetic energy
define potential energy
store energy within system (function of order) bricks to wall analogy
define kinetic enegry
energy in process of doing work
order and potential energy?
AB —— A+B
AB has more order and more potential energy
A+B has less order less potential energy
2 ways to get energetic reaction started
1- increase temperature
2-lower activation energy with catalyst(enzyme)
define cofactors
inorganic compound required to bind to enzyme to activate site
define coenzyme
organic compound required to bond to enzyme to activate site
how to get
C+D ———CD
need energy and enzyme
define cellular respiration (aerobic)
breakdown of organic molecules into CO2 and H2O, releasing their energy +using energy to make energy carrying molecule called adenosine triphosphate(ATP)
define phosphorication
adding phosphate to something
4 processes in cell respiration
glycolysis, pyruvatcoxidation, kreb cycle/citric acid cycle, oxidation phosphorylation
what 5 things should you know about cell respiration proccesses?
- where it occurs
- what it starts with
- what you should get out of it(product)
- function of named molecules
- overall picture (making ATP)
define glycolysis and should know?
occurs in cytoplasm. breaking down sugar.
1. occurs in cytoplasm
2. starts with glucose 2 ADP+ 2Pi, 2NAD+
3. get 2 pyruvate, 2ATP,2NADH
4. function high energy
5. to make ATP
define nicotinemide adeninic dinuchotide
NAD. bonds to H to get NADH which is a high energy elctron carrier.
define pyruvate oxidations and should know?
- Mitochondrial matrix
- 3C pyruvate +CoA, NAD+
- 2C Acetal CoA, CO2, NADH
- prepares it to move into citric acid cycle
- make ATP with exer and endo process
define citric acid cycle and should know
- matrix
- 2C acetal CoA, 1ADP+Pi, 3NAD+, FAD
- ATP, 3NADH, FADH2, 2CO2, CoA
- make NADH
5.make ATP, NADH, FADH2
define oxidation phosphorylation and should know
- inner membrane of mito
2.
2 steps of oxydative phosphorylation
generate proton motive force and chemiosmosis
define proton motive force
take high enegry electrons from NADH and FADH2, pass down electron transport chain (high to low energy state) so it releases energy used to pump H+ ions from matrix into inner membrane space creating [H+] gradient
define chemiosmosis
energy from proton motive force used to phosphorylate ADP
how mamy ATP and NADH from glucose
6NADH- 18 ATP
36 ATP MAX(if none used)
29 ATP most times
anerobic respiration by who and how?
bacteria using non- O2 final electron acceptor(SO4, NO3) that create cellular energy
define fermentation
ATP production through glycolysis by oxidizing NADH. the breaking down of sugar molecules into simpler compounds to produce substances that can be used in making chemical energy
2 types of fermentation
alcoholic fermentation and non alcoholic fermentation
define alcoholic fermentation
start with Glucose+ 2NAD+, 2ADP+Pi then use glycolysis to get 2NADH, 2 pyruvate, 2 ATP then break off CO2 while making ethanol , break 2NADH to 2NAD+ so we can redo cycle
define nonalcoholic fermentation
start with glucose +2NAD+, 2ADP+2Pi then do glycolysis and get 2NADH, 2 pyruvate, 2ATP then turn that into lactic acid (3C) then turn 2NADH into 2NAD+ to do cycle again(HUMANS DO WHEN USING MUSCLES W/O ENOUGH OXYGEN)
define hydrolysis
using water to break molecules
define lipid metabolism
triglycerides->+3H2O(hydrolysis)->3 long chain fatty acids +glycerol(3C) where ATP turns to ADP making it 3C-1P
glucose vs fats
polar. nonpolar
small. large
4k(killa)cal/g. 9kcal/g
define protien metabolism
dietary protien- digested-> amino acids(-excess->energy)->make protiens
3 approaches to removing NH3(nitrogenous waste aka amonia)
- fish/some amphibians/aquatic insects- bacteria clean out of water since its their food
- mammal/some amphibians- NH3+H2O-> urea. nontoxic soluble in H2O
- reptile/birds/terrestrial insects-NH3->urea-> uric acid(pasty solid) dont want to waste water
2 approaches to regulation of metabolic process
simple equilibrium dynamics and feedback inhibition
define simple equilibrium dynamics
AB(->/<- with enzyme)A+B. (equilibrium reaction) if add AB make more A+B. remove A+B make more AB. remove A and/or B shift right
state of balance between opposing forces where a system can operate at its best by adjusting to achieve its desired outcome
define feedback inhibition
at [high] product binds to inhibition site on an enzyme and lowers its affinity to reactants so more of it needed and reaction is slowed
photosynthesis formula and what is opposite?
6H2O+6CO2-light->C6H12O6(glucose)+6O2
opposite is cellular respiration
2 major steps of photosynthesis
light dependent reactions and light independent reactions
define light dependent reactions
(LDR) energy from light is captured and used to create ATP+NADPH
define light independent reaction and formula
(LIR)-ATP+NADPH+CO2-> sugar
use stored chemical energy from the light-dependent reactions to “fix” CO2 and create a product that can be converted into glucose
what is photosynthesis done by?
plants, algae, photosynthesis bacteria, and cyanobacteria
light definition
electromagnetic energy traveling in packets of enegry (photon) that move in waves (visible-400nm-740nm)
how can photon become energy source?
it must strike electron in photosynthesis pigment molecule
how is photon of light reflected or absorbed in pigment molecule?
reflected if frequency of photon is not equal to frequency of electron, absorbed of frequency of photon does equal frequency of electron. generates high energy electron=excited electron
main photosynthetic pigment? used by who?
chlorophyll A- 420nm(red)+680nm(blue) by all eukaryotes +cyanobacteria
define absorption spectrum
wavelengths of light used by specific pigment
define action spectrum
total light used by plant. which wavelengths of light are most effective in driving a biological process
where light dependent reactions happen
on thylakoid membrane or on membrane of prokaryotes in photosystems
define photosystems
clusters of photosynthetic pigments imbedded in TMP. have a reaction center chlorophyll which generates excited e-. and antenna complex which captures light energy+transports to rxn center chlorophyll
3 known photosystems
P870-photosynthetic bacteria
P680-all eukaryotes(photosystem 2)
P700- all cyanobacteria(photosystem 1)
2 versions of LDR
cyclic photophosphorylation and noncyclic photophosphorylation
define cyclic photophosphorylation and product
light strikes antenna complex of P870,energy is absorbed, energy is transferred to RCC, which generates 1excited e-, e- to ETC, ETC takes electron to low energy stage on P870, energy is used to pump H across membrane generating a proton motive force
ATP
define noncyclic photophosphorylation and 2 products
light strikes AC in P680, energy->RCC, RCC passes two excited e- -> ETC, energy also used to split water generating both ATP and NADPH, ETC-> e- to low energy state on P700, light in P700 generates two excited e-
ATP and NADPH
define great oxidation event
4.5 bill yrs planet->3.3 billyrs first life(in rocks) NO EVIDENCE OF OXYGEN->2.7 billyrs first cyanobacteria-> 2.2 billyrs eukaryote fossils found so cyano made enough O2 to be breathable at this time
define carbon fixation
converting inorganic carbon into organic
light independent reaction(calvin cycle) formula and relation to carbon
6CO2+12NADPH+18ATP->C6H12O6
every turn of calvin cycle fixes one carbon atom
exergonic reaction vs endergonic
An exergonic reaction releases energy and is considered spontaneous, while an endergonic reaction absorbs energy and is non-spontaneous, meaning it requires an external energy input to proceed
describe graph of exergonic energy
y axis- potential energy
xaxis- time
top left starts AB which experiences a catalyst making a hump of activation energy in line then continues back down to bottom right where A+B is
define activation energy
amount of enegry needed to make a reaction occur
define enzyme substrate complex
when enzyme bonds with a substance
describe process of AB -> A+B
AB binds to active site of ABace enzyme with coenzyme or cofactor. ABace will change shape and stress bond btwn AB until bond breaks and enzyme loses affinity to A+B and enzyme and A+B separate
describe graph of endergonic reaction
top left AB to downslide of A+B while crossing paths of bottom lefts C+D line upslide to CD. and endergonic rxn needs exer to occur
define cell respirations graph
top left organic molecule downslides to CO2+ H2O while crossing path of bottom lefts many ADP+Pi upslide to many ATP
energy requiring process graph
top left many ATP downslides to many ADP+Pi while crossing line of bottom lefts relaxed muscle upslide to contracted muscle
how many ATP per 1NADH and 1FADH2
1NADH-> 3ATP
1FADH2-> 2ATP
reduction vs oxidation
reduction-gain of electrons
oxidation-loss of electrons
diff btwn absorbtiom spectrum and action spectrum
Absorption Spectrum: Describes the wavelengths that are absorbed or the light that is harvested.
Action Spectrum: Describes the wavelengths that actually drive photosynthesis.