SNPs

Question 1

SNPs use

Answer 1

degraded samples, ethnic origin/physical traits

Question 2

Advantages of SNPs

Answer 2

no stutter
can use to predict ethnicity/physical traits
lower mutation rate (than STRs)

Question 3

Disadvantages of SNPs

Answer 3

need around 50 SNPs to get the RMP of the core 13 STRs

currently no common loci

Question 4

Chip-based methods

Answer 4

DNA of known sequence attached in known place
DNA of unknown sequence with fluorescent probe added
Complementary then binds and fluoresces

Question 5

TaqMan assay

Answer 5

add DNA probe with Q and R dye attached to PCR with special pol
if it binds then the pol breaks down probe and it amplifies and Q and R separate

Question 6

Direct sequencing

Answer 6

PCR sample as usual
use ExoSAP to destroy remaining primers and dNTPs
PCR again with 1 primer and both dNTPs and ddNTPs
use electrophoresis to separate different sized pieces
look at fluorescence color to determine nucleotide

Question 7

Minisequencing (SNaPshot)

Answer 7

same concept as direct sequencing but primers are designed to anneal directly before SNP and use only ddNTPs