Database Analysis

Question 1

Population databases purpose

Answer 1

provide genotype and allele frequencies for estimating random match probabilities

Question 2

Creating databases gathering samples

Answer 2

local bloodbanks/hospitals
no personal identification
hopefully unrelated and random
self-identify racial/ethnic profile
usually >100 per group
need IRB (institutional review board) approval

Question 3

Allele frequency

Answer 3

allele count/2*people sampled

Question 4

Genotype frequency

Answer 4

genotype count/people sampled

Question 5

Minimum allele frequency

Answer 5

observed allele 5 times for reliable frequency estimate <5, inflated frequency used in calculations to be conservative
5/2N

Question 6

Allele/genotype frequencies

Answer 6

not greatly affected by increasing sample size above a few hundred
broad racial/ethnic categories usually sufficient
unless working with a small, isolated group CODIS PopStats allele frequencies likely sufficient

Question 7

HWE and LE tests

Answer 7

before calculating probabilities, check for deviations from HWE and LE
non-independence of alleles or loci
null alleles

Question 8

Statistical packages

Answer 8

GENEPOP
DNATYPE
arlequin
power marker
PopStats (CODIS)

Question 9

Summary statistics

Answer 9

h- expected homozygosity
H- expected heterozygosity
ne- effective number of alleles
PD- power of discrimination
PE- power of exclusion